product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD314 (NKG2D)
catalog :
320814
quantity :
1 mg
price :
468 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1D11
reactivity :
African green monkey, human, mouse
application :
immunoprecipitation, flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 19
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 4a
Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Ingegnere T, Mariotti F, Pelosi A, Quintarelli C, De Angelis B, Tumino N, et al. Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing. Front Immunol. 2019;10:957 pubmed publisher
  • flow cytometry; human; loading ...; fig s1d
Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
  • mass cytometry; human; loading ...; fig 4f
Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
  • flow cytometry; human; loading ...; fig 5c
Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
  • flow cytometry; human; loading ...; fig s6a
Delso Vallejo M, Kollet J, Koehl U, Huppert V. Influence of Irradiated Peripheral Blood Mononuclear Cells on Both Ex Vivo Proliferation of Human Natural Killer Cells and Change in Cellular Property. Front Immunol. 2017;8:854 pubmed publisher
  • flow cytometry; human; loading ...; fig 4e
Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
  • flow cytometry; mouse; fig 5b
Tripathi D, Venkatasubramanian S, Cheekatla S, Paidipally P, Welch E, Tvinnereim A, et al. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nat Commun. 2016;7:13896 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Zanetti S, Ziblat A, Torres N, Zwirner N, Bouzat C. Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells. J Biol Chem. 2016;291:16541-52 pubmed publisher
  • blocking or activating experiments; human; fig 8
Rancan C, Schirrmann L, Hüls C, Zeidler R, Moosmann A. Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells. PLoS Pathog. 2015;11:e1004906 pubmed publisher
  • flow cytometry; African green monkey
Watanabe M, Kudo Y, Kawano M, Nakayama M, Nakamura K, Kameda M, et al. NKG2D functions as an activating receptor on natural killer cells in the common marmoset (Callithrix jacchus). Int Immunol. 2014;26:597-606 pubmed publisher
Höfle J, Trenkner T, Kleist N, Schwane V, Vollmers S, Barcelona B, et al. Engagement of TRAIL triggers degranulation and IFNγ production in human natural killer cells. EMBO Rep. 2022;:e54133 pubmed publisher
Van der Meer J, de Jonge P, van der Waart A, Geerlings A, Moonen J, Brummelman J, et al. CD34+ progenitor-derived NK cell and gemcitabine combination therapy increases killing of ovarian cancer cells in NOD/SCID/IL2Rgnull mice. Oncoimmunology. 2021;10:1981049 pubmed publisher
Dudek M, Pfister D, Donakonda S, Filpe P, Schneider A, Laschinger M, et al. Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH. Nature. 2021;592:444-449 pubmed publisher
Thrash E, Kleinsteuber K, Hathaway E, Nazzaro M, Haas E, Hodi F, et al. High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples. STAR Protoc. 2020;1:100055 pubmed publisher
Breman E, Demoulin B, Agaugué S, Mauen S, Michaux A, Springuel L, et al. Overcoming Target Driven Fratricide for T Cell Therapy. Front Immunol. 2018;9:2940 pubmed publisher
Glasner A, Isaacson B, Mandelboim O. Expression and function of NKp46 W32R: the human homologous protein of mouse NKp46 W32R (Noé). Sci Rep. 2017;7:40944 pubmed publisher
Correia D, Fogli M, Hudspeth K, da Silva M, Mavilio D, Silva Santos B. Differentiation of human peripheral blood V?1+ T cells expressing the natural cytotoxicity receptor NKp30 for recognition of lymphoid leukemia cells. Blood. 2011;118:992-1001 pubmed publisher
Kraetzel K, Stoelcker B, Eissner G, Multhoff G, Pfeifer M, Holler E, et al. NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells. Eur Respir J. 2008;32:563-70 pubmed publisher
product information
Antigen :
CD314
Apps. Abbrev. :
FC, IP, FA
Cat # :
320814
Clone :
1D11
Item :
Ultra-LEAF™ Purified anti-human CD314 (NKG2D)
Isotype :
Mouse IgG1, κ
Other Names :
NKG2D
Size :
1 mg
Price (USD) :
468 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Application Notes :
The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation^1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation^2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA