LEAF™ Purified anti-human CD314 (NKG2D) | BioLegend 320809 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD314 (NKG2D)

catalog :

320809

quantity :

50 μg

price :

130 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D11

reactivity :

human, mouse

application :

immunoprecipitation, flow cytometry, blocking or activating experiments, mass cytometry

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 4a	Meckiff B, Ladell K, McLaren J, Ryan G, Leese A, James E, et al. Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells. J Immunol. 2019;203:1276-1287 pubmed publisher
flow cytometry; human; loading ...; fig 2a	Ingegnere T, Mariotti F, Pelosi A, Quintarelli C, De Angelis B, Tumino N, et al. Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing. Front Immunol. 2019;10:957 pubmed publisher
flow cytometry; human; loading ...; fig s1d	Dias J, Boulouis C, Gorin J, van den Biggelaar R, Lal K, Gibbs A, et al. The CD4-CD8- MAIT cell subpopulation is a functionally distinct subset developmentally related to the main CD8+ MAIT cell pool. Proc Natl Acad Sci U S A. 2018;115:E11513-E11522 pubmed publisher
mass cytometry; human; loading ...; fig 4f	Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
flow cytometry; human; loading ...; fig 5c	Salio M, Gasser O, González López C, Martens A, Veerapen N, Gileadi U, et al. Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells. J Immunol. 2017;199:2631-2638 pubmed publisher
flow cytometry; human; loading ...; fig s6a	Delso Vallejo M, Kollet J, Koehl U, Huppert V. Influence of Irradiated Peripheral Blood Mononuclear Cells on Both Ex Vivo Proliferation of Human Natural Killer Cells and Change in Cellular Property. Front Immunol. 2017;8:854 pubmed publisher
flow cytometry; human; loading ...; fig 4e	Domae E, Hirai Y, Ikeo T, Goda S, Shimizu Y. Cytokine-mediated activation of human ex vivo-expanded V?9V?2 T cells. Oncotarget. 2017;8:45928-45942 pubmed publisher
flow cytometry; mouse; fig 5b	Tripathi D, Venkatasubramanian S, Cheekatla S, Paidipally P, Welch E, Tvinnereim A, et al. A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice. Nat Commun. 2016;7:13896 pubmed publisher
flow cytometry; human; loading ...; fig 5a	Zanetti S, Ziblat A, Torres N, Zwirner N, Bouzat C. Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells. J Biol Chem. 2016;291:16541-52 pubmed publisher
blocking or activating experiments; human; fig 8	Rancan C, Schirrmann L, Hüls C, Zeidler R, Moosmann A. Latent Membrane Protein LMP2A Impairs Recognition of EBV-Infected Cells by CD8+ T Cells. PLoS Pathog. 2015;11:e1004906 pubmed publisher
	Watanabe M, Kudo Y, Kawano M, Nakayama M, Nakamura K, Kameda M, et al. NKG2D functions as an activating receptor on natural killer cells in the common marmoset (Callithrix jacchus). Int Immunol. 2014;26:597-606 pubmed publisher
	Correia D, Fogli M, Hudspeth K, da Silva M, Mavilio D, Silva Santos B. Differentiation of human peripheral blood V?1+ T cells expressing the natural cytotoxicity receptor NKp30 for recognition of lymphoid leukemia cells. Blood. 2011;118:992-1001 pubmed publisher
	Kraetzel K, Stoelcker B, Eissner G, Multhoff G, Pfeifer M, Holler E, et al. NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells. Eur Respir J. 2008;32:563-70 pubmed publisher

product information

Antigen :

CD314

Apps. Abbrev. :

Flow Cytometry, IP, FA

Cat # :

320809

Clone :

1D11

Item :

LEAF™ Purified anti-human CD314 (NKG2D)

Isotype :

Mouse IgG1, κ

Other Names :

NKG2D

Size :

50 μg

Price (USD) :

130 USD

Reactivity :

Human, African Green, Baboon, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation 1,2 , blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation 2-7 . The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 320810). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).