Babco, Signet, Sternberger Monoclonals, Senetek, Covance

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Dry Human AIM Assay Cocktail Kit

320305

5 tests

198 USD

320305

Dry Human AIM Assay Cocktail Kit

Dry Reagents, Dry Cocktails

5 tests

198 USD

Human

DRY_COCKTAIL

Dry Human AIM (Activation Induced Markers) Assay Cocktail kit is a dried down cocktail kit that aims to characterize activated T cells subsets. Components: 1 pouch containing 5 tests of Dry Human AIM Assay Cocktail (1 test/tube) 1 set of Compensation Control with lot-matched antibodiesRequired Materials Not Included:Cell Stain Buffer for Wash Steps (Cat. No. 420201 or similar)1x RBC Lysis Buffer diluted from 10x RBC Lysis Buffer (Cat. No. 420302 or similar)1% diluted Fixation Buffer from 4% if needed (Cat. No. 420801 or similar)Assay Protocol:;Open the Dry Human AIM Assay Cocktail Kit and the respective compensation control pouches and set up the dried down cocktail tubes to start the experiment;Add 100 μL of human peripheral blood or similar cell suspension of interest into each of the dried down cocktail tubes and respective compensation controls and vortex thoroughly;Incubate the samples at room temperature for 15 minutes;If the cell suspension used does not need lysing, proceed to step 7. If performing Lyse wash whole blood, add 2 mL of 1x of RBC Lysis Buffer (Cat. No. 420302 or similar) to each tube and vortex thoroughly;Incubate the samples at room temperature for 15 minutes;Centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet.;Add 2 mL Cell Stain Buffer (Cat. No. 420201 or similar) and centrifuge the samples at 500 xg for 5 minutes, decant and vortex to loosen the pellet.;Repeat step 7.;Add 300 - 500 µL Cell Stain Buffer or 1% diluted Fixation Buffer (Cat. No. 420801 or similar) and acquire the samples using a flow cytometer.;