product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-human FOXP3
catalog :
320114
quantity :
100 tests
price :
370 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF647
clone name :
206D
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 44
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig s1l
Pasciuto E, Burton O, Roca C, Lagou V, Rajan W, Theys T, et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition. Cell. 2020;182:625-640.e24 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 3
Herrera Rios D, Mughal S, Teuber Hanselmann S, Pierscianek D, Sucker A, Jansen P, et al. Macrophages/Microglia Represent the Major Source of Indolamine 2,3-Dioxygenase Expression in Melanoma Metastases of the Brain. Front Immunol. 2020;11:120 pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Ondigo B, Ndombi E, Nicholson S, Oguso J, Carter J, Kittur N, et al. Functional Studies of T Regulatory Lymphocytes in Human Schistosomiasis in Western Kenya. Am J Trop Med Hyg. 2018;98:1770-1781 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
  • immunohistochemistry; human; loading ...; fig 3a
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
  • flow cytometry; human; loading ...; fig 4d
Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, et al. CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus. Front Immunol. 2016;7:512 pubmed
  • flow cytometry; human; loading ...; fig 1b
Wei C, Mei J, Tang L, Liu Y, Li D, Li M, et al. 1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis. Cell Death Dis. 2016;7:e2489 pubmed publisher
  • flow cytometry; human; fig s2
Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
Xiao X, Cheng Y, Zheng X, Fang Y, Zhang Y, Sun R, et al. Bispecific NK-cell engager targeting BCMA elicits stronger antitumor effects and produces less proinflammatory cytokines than T-cell engager. Front Immunol. 2023;14:1113303 pubmed publisher
Herter J, Kiljan M, Kunze S, Reinscheid M, Ibruli O, Cai J, et al. Influence of chemoradiation on the immune microenvironment of cervical cancer patients. Strahlenther Onkol. 2023;199:121-130 pubmed publisher
Riese P, Trittel S, Akmatov M, May M, Prokein J, Illig T, et al. Distinct immunological and molecular signatures underpinning influenza vaccine responsiveness in the elderly. Nat Commun. 2022;13:6894 pubmed publisher
Weaver J, Stack E, Bugg xe9 J, Hu C, McGrath L, Mueller A, et al. Differential expression of CCR8 in tumors versus normal tissue allows specific depletion of tumor-infiltrating T regulatory cells by GS-1811, a novel Fc-optimized anti-CCR8 antibody. Oncoimmunology. 2022;11:2141007 pubmed publisher
Parasar P, Bernard M, Ahn S, Kshirsagar S, Nguyen S, Grzesiak G, et al. Isolation and characterization of uterine leukocytes collected using a uterine swab technique. Am J Reprod Immunol. 2022;88:e13614 pubmed publisher
Glaubitz J, Wilden A, Golchert J, Homuth G, V xf6 lker U, Br xf6 ker B, et al. In mouse chronic pancreatitis CD25+FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response. Nat Commun. 2022;13:4502 pubmed publisher
Hui Z, Zhang J, Ren Y, Li X, Yan C, Yu W, et al. Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC). Cell Death Dis. 2022;13:607 pubmed publisher
Capelle C, Cir xe9 S, Domingues O, Ernens I, Hedin F, Fischer A, et al. Combinatorial analysis reveals highly coordinated early-stage immune reactions that predict later antiviral immunity in mild COVID-19 patients. Cell Rep Med. 2022;3:100600 pubmed publisher
Altorki N, Borczuk A, Harrison S, Groner L, Bhinder B, Mittal V, et al. Global evolution of the tumor microenvironment associated with progression from preinvasive invasive to invasive human lung adenocarcinoma. Cell Rep. 2022;39:110639 pubmed publisher
Chen B, Scurrah C, McKinley E, Simmons A, Ramirez Solano M, Zhu X, et al. Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell. 2021;184:6262-6280.e26 pubmed publisher
Verma A, Hawes C, Lakshmanappa Y, Roh J, Schmidt B, Dutra J, et al. Monoclonal antibodies protect aged rhesus macaques from SARS-CoV-2-induced immune activation and neuroinflammation. Cell Rep. 2021;37:109942 pubmed publisher
Shen X, Zhang H, Xie H, Chen L, Li S, Zheng J, et al. Reduced CCR6+IL-17A+Treg Cells in Blood and CCR6-Dependent Accumulation of IL-17A+Treg Cells in Lungs of Patients With Allergic Asthma. Front Immunol. 2021;12:710750 pubmed publisher
Tian M, Hao F, Jin X, Sun X, Jiang Y, Wang Y, et al. ACLY ubiquitination by CUL3-KLHL25 induces the reprogramming of fatty acid metabolism to facilitate iTreg differentiation. elife. 2021;10: pubmed publisher
Vanderbeke L, Van Mol P, van Herck Y, De Smet F, Humblet Baron S, Martinod K, et al. Monocyte-driven atypical cytokine storm and aberrant neutrophil activation as key mediators of COVID-19 disease severity. Nat Commun. 2021;12:4117 pubmed publisher
Moysi E, Del Rio Estrada P, Torres Ruiz F, Reyes Teran G, Koup R, Petrovas C. In Situ Characterization of Human Lymphoid Tissue Immune Cells by Multispectral Confocal Imaging and Quantitative Image Analysis; Implications for HIV Reservoir Characterization. Front Immunol. 2021;12:683396 pubmed publisher
Yang Y, Li Y, Wang Y, Ruan G, Tian C, Wang Q, et al. The effects of BMMSC treatment on lung tissue degeneration in elderly macaques. Stem Cell Res Ther. 2021;12:156 pubmed publisher
Xu Monette Z, Xiao M, Au Q, Padmanabhan R, Xu B, Hoe N, et al. Immune Profiling and Quantitative Analysis Decipher the Clinical Role of Immune-Checkpoint Expression in the Tumor Immune Microenvironment of DLBCL. Cancer Immunol Res. 2019;: pubmed publisher
Lagou V, Garcia Perez J, Smets I, Van Horebeek L, Vandebergh M, Chen L, et al. Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators. Cell Rep. 2018;25:798-810.e6 pubmed publisher
Adel Patient K, Lezmi G, Castelli F, Blanc S, Bernard H, Soulaines P, et al. Deep analysis of immune response and metabolic signature in children with food protein induced enterocolitis to cow's milk. Clin Transl Allergy. 2018;8:38 pubmed publisher
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, et al. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Clin Cancer Res. 2015;21:2840-50 pubmed publisher
Hodi F, Lawrence D, Lezcano C, Wu X, Zhou J, Sasada T, et al. Bevacizumab plus ipilimumab in patients with metastatic melanoma. Cancer Immunol Res. 2014;2:632-42 pubmed publisher
Nigam P, Velu V, Kannanganat S, Chennareddi L, Kwa S, Siddiqui M, et al. Expansion of FOXP3+ CD8 T cells with suppressive potential in colorectal mucosa following a pathogenic simian immunodeficiency virus infection correlates with diminished antiviral T cell response and viral control. J Immunol. 2010;184:1690-701 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Nevala W, Vachon C, Leontovich A, Scott C, Thompson M, Markovic S. Evidence of systemic Th2-driven chronic inflammation in patients with metastatic melanoma. Clin Cancer Res. 2009;15:1931-9 pubmed publisher
Raghavan S, Cao D, Widhe M, Roth K, Herrath J, Engstrom M, et al. FOXP3 expression in blood, synovial fluid and synovial tissue during inflammatory arthritis and intra-articular corticosteroid treatment. Ann Rheum Dis. 2009;68:1908-15 pubmed publisher
Grant J, Bourcier K, Wallace S, Pan D, Conway A, Seyfert Margolis V, et al. Validated protocol for FoxP3 reveals increased expression in type 1 diabetes patients. Cytometry B Clin Cytom. 2009;76:69-78 pubmed publisher
Zheng Y, Manzotti C, Burke F, Dussably L, Qureshi O, Walker L, et al. Acquisition of suppressive function by activated human CD4+ CD25- T cells is associated with the expression of CTLA-4 not FoxP3. J Immunol. 2008;181:1683-91 pubmed
Jiang Q, Zhang L, Wang R, Jeffrey J, Washburn M, Brouwer D, et al. FoxP3+CD4+ regulatory T cells play an important role in acute HIV-1 infection in humanized Rag2-/-gammaC-/- mice in vivo. Blood. 2008;112:2858-68 pubmed publisher
Kavanagh B, O Brien S, Lee D, Hou Y, Weinberg V, Rini B, et al. CTLA4 blockade expands FoxP3+ regulatory and activated effector CD4+ T cells in a dose-dependent fashion. Blood. 2008;112:1175-83 pubmed publisher
Zonios D, Falloon J, Bennett J, Shaw P, Chaitt D, Baseler M, et al. Idiopathic CD4+ lymphocytopenia: natural history and prognostic factors. Blood. 2008;112:287-94 pubmed publisher
Pillai V, Karandikar N. Attack on the clones? Human FOXP3 detection by PCH101, 236A/E7, 206D, and 259D reveals 259D as the outlier with lower sensitivity. Blood. 2008;111:463-4; author reply 464-6 pubmed
Hartigan O Connor D, Abel K, McCune J. Suppression of SIV-specific CD4+ T cells by infant but not adult macaque regulatory T cells: implications for SIV disease progression. J Exp Med. 2007;204:2679-92 pubmed
Tran D, Ramsey H, Shevach E. Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype. Blood. 2007;110:2983-90 pubmed
Gao Q, Qiu S, Fan J, Zhou J, Wang X, Xiao Y, et al. Intratumoral balance of regulatory and cytotoxic T cells is associated with prognosis of hepatocellular carcinoma after resection. J Clin Oncol. 2007;25:2586-93 pubmed
Hartigan O Connor D, Poon C, Sinclair E, McCune J. Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells. J Immunol Methods. 2007;319:41-52 pubmed publisher
Liu W, Putnam A, Xu Yu Z, Szot G, Lee M, Zhu S, et al. CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells. J Exp Med. 2006;203:1701-11 pubmed
product information
Antigen :
FOXP3
Apps. Abbrev. :
ICFC
Cat # :
320114
Clone :
206D
Item :
Alexa Fluor® 647 anti-human FOXP3
Isotype :
Mouse IgG1, κ
Other Names :
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Size :
100 tests
Price (USD) :
370 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A647
Immunogen :
Full-length FOXP3 protein
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA