product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-human CD4
catalog :
317450
quantity :
100 μg
price :
229 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
OKT4
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig e1a, 3h
Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 1c
Hoang T, Harper J, Pino M, Wang H, Micci L, King C, et al. Bone Marrow-Derived CD4+ T Cells Are Depleted in Simian Immunodeficiency Virus-Infected Macaques and Contribute to the Size of the Replication-Competent Reservoir. J Virol. 2019;93: pubmed publisher
Zhou P, Song G, Liu H, Yuan M, He W, Beutler N, et al. Broadly neutralizing anti-S2 antibodies protect against all three human betacoronaviruses that cause deadly disease. Immunity. 2023;56:669-686.e7 pubmed publisher
Zhang M, Chong K, Chen Z, Guo H, Liu Y, Kang Y, et al. Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes. JCI Insight. 2023;8: pubmed publisher
Gilman K, Cracchiolo M, Matiatos A, Davini D, Simpson R, Katsanis E. Partially replacing cyclophosphamide with bendamustine in combination with cyclosporine A improves survival and reduces xenogeneic graft-versus-host-disease. Front Immunol. 2022;13:1045710 pubmed publisher
Wang P, Mo Z, Zhang Y, Guo C, Chikede T, Chen D, et al. Serum IL-5 levels predict HBsAg seroclearance in patients treated with Nucleos(t)ide analogues combined with pegylated interferon. Front Immunol. 2022;13:1104329 pubmed publisher
Seenappa L, Jakubowski A, Steinbuck M, Palmer E, Haqq C, Carter C, et al. Amphiphile-CpG vaccination induces potent lymph node activation and COVID-19 immunity in mice and non-human primates. NPJ Vaccines. 2022;7:128 pubmed publisher
Pino M, Pagliuzza A, Pampena M, Deléage C, Viox E, Nguyen K, et al. Limited impact of fingolimod treatment during the initial weeks of ART in SIV-infected rhesus macaques. Nat Commun. 2022;13:5055 pubmed publisher
He W, Yuan M, Callaghan S, Musharrafieh R, Song G, Silva M, et al. Broadly neutralizing antibodies to SARS-related viruses can be readily induced in rhesus macaques. Sci Transl Med. 2022;14:eabl9605 pubmed publisher
Yuan X, Sun L, Jeske R, Nkosi D, York S, Liu Y, et al. Engineering extracellular vesicles by three-dimensional dynamic culture of human mesenchymal stem cells. J Extracell Vesicles. 2022;11:e12235 pubmed publisher
Sureshchandra S, Zulu M, Doratt B, Jankeel A, Tifrea D, Edwards R, et al. Single-cell RNA sequencing reveals immunological rewiring at the maternal-fetal interface following asymptomatic/mild SARS-CoV-2 infection. Cell Rep. 2022;39:110938 pubmed publisher
He W, Musharrafieh R, Song G, Dueker K, Tse L, Martinez D, et al. Targeted isolation of diverse human protective broadly neutralizing antibodies against SARS-like viruses. Nat Immunol. 2022;23:960-970 pubmed publisher
Wang H, Cao K, Liu S, Xu Y, Tang L. Tim-3 Expression Causes NK Cell Dysfunction in Type 2 Diabetes Patients. Front Immunol. 2022;13:852436 pubmed publisher
Wu B, Zhang X, Chiang H, Pan H, Yuan B, Mitra P, et al. RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. Nat Commun. 2022;13:2155 pubmed publisher
Jorapur A, Marshall L, Jacobson S, Xu M, Marubayashi S, Zibinsky M, et al. EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22. PLoS Pathog. 2022;18:e1010200 pubmed publisher
Shen X, Zhang H, Xie H, Chen L, Li S, Zheng J, et al. Reduced CCR6+IL-17A+Treg Cells in Blood and CCR6-Dependent Accumulation of IL-17A+Treg Cells in Lungs of Patients With Allergic Asthma. Front Immunol. 2021;12:710750 pubmed publisher
Leclercq G, Haegel H, Schneider A, Giusti A, Marrer Berger E, Boetsch C, et al. Src/lck inhibitor dasatinib reversibly switches off cytokine release and T cell cytotoxicity following stimulation with T cell bispecific antibodies. J Immunother Cancer. 2021;9: pubmed publisher
Ho C, Silva A, Tomita B, Weng H, Ho I. Differential impacts of TNFα inhibitors on the transcriptome of Th cells. Arthritis Res Ther. 2021;23:199 pubmed publisher
Richert Spuhler L, MAR C, Shinde P, Wu F, Hong T, Greene E, et al. CD101 genetic variants modify regulatory and conventional T cell phenotypes and functions. Cell Rep Med. 2021;2:100322 pubmed publisher
Zhou X, Ma F, Xie J, Yuan M, Li Y, Shaabani N, et al. Diverse immunoglobulin gene usage and convergent epitope targeting in neutralizing antibody responses to SARS-CoV-2. Cell Rep. 2021;35:109109 pubmed publisher
Mathewson N, Ashenberg O, Tirosh I, Gritsch S, Perez E, Marx S, et al. Inhibitory CD161 receptor identified in glioma-infiltrating T cells by single-cell analysis. Cell. 2021;184:1281-1298.e26 pubmed publisher
Hoang T, Pino M, Boddapati A, Viox E, Starke C, Upadhyay A, et al. Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques. Cell. 2021;184:460-475.e21 pubmed publisher
Hoang T, Pino M, Boddapati A, Viox E, Starke C, Upadhyay A, et al. Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques. bioRxiv. 2020;: pubmed publisher
Song G, He W, Callaghan S, Anzanello F, Huang D, Ricketts J, et al. Cross-reactive serum and memory B cell responses to spike protein in SARS-CoV-2 and endemic coronavirus infection. bioRxiv. 2020;: pubmed publisher
Kan S, Bito T, Shimabuku M, Taguchi J, Ohkusa T, Shimodaira S, et al. Impact of mature dendritic cells pulsed with a novel WT1 helper peptide on the induction of HLA‑A2‑restricted WT1‑reactive CD8+ T cells. Int J Oncol. 2020;57:1047-1056 pubmed publisher
Zhao F, Joyce C, Burns A, Nogal B, Cottrell C, Ramos A, et al. Mapping Neutralizing Antibody Epitope Specificities to an HIV Env Trimer in Immunized and in Infected Rhesus Macaques. Cell Rep. 2020;32:108122 pubmed publisher
Bäcker Koduah P, Infante Duarte C, Ivaldi F, Uccelli A, Bellmann Strobl J, Wernecke K, et al. Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS. Ann Clin Transl Neurol. 2020;: pubmed publisher
Pino M, Uppada S, Pandey K, King C, Nguyen K, Shim I, et al. Safety and Immunological Evaluation of Interleukin-21 Plus Anti-α4β7 mAb Combination Therapy in Rhesus Macaques. Front Immunol. 2020;11:1275 pubmed publisher
Chen Y, Yin J, Li W, Li H, Chen D, Zhang C, et al. Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma. Cell Res. 2020;: pubmed publisher
Su C, Johnson M, Torres A, Thomas R, Manduchi E, SHARMA P, et al. Mapping effector genes at lupus GWAS loci using promoter Capture-C in follicular helper T cells. Nat Commun. 2020;11:3294 pubmed publisher
Kim N, Kim H, Lee K, Hong Y, Cho J, Choi J, et al. Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma. Nat Commun. 2020;11:2285 pubmed publisher
Harper J, Gordon S, Chan C, Wang H, Lindemuth E, Galardi C, et al. CTLA-4 and PD-1 dual blockade induces SIV reactivation without control of rebound after antiretroviral therapy interruption. Nat Med. 2020;26:519-528 pubmed publisher
García Broncano P, Medrano L, Berenguer J, Brochado Kith O, Gonz xe1 lez Garc xed a J, Jim xe9 nez Sousa M, et al. Mild profile improvement of immune biomarkers in HIV/HCV-coinfected patients who removed hepatitis C after HCV treatment: A prospective study. J Infect. 2020;80:99-110 pubmed publisher
Yost K, Satpathy A, Wells D, Qi Y, Wang C, Kageyama R, et al. Clonal replacement of tumor-specific T cells following PD-1 blockade. Nat Med. 2019;25:1251-1259 pubmed publisher
Franco L, Gadkari M, Howe K, Sun J, Kardava L, Kumar P, et al. Immune regulation by glucocorticoids can be linked to cell type-dependent transcriptional responses. J Exp Med. 2019;216:384-406 pubmed publisher
Sampedro Nuñez M, Serrano Somavilla A, Adrados M, Cameselle Teijeiro J, Blanco Carrera C, Cabezas Agricola J, et al. Analysis of expression of the PD-1/PD-L1 immune checkpoint system and its prognostic impact in gastroenteropancreatic neuroendocrine tumors. Sci Rep. 2018;8:17812 pubmed publisher
Jelcić I, Al Nimer F, Wang J, Lentsch V, Planas R, Jelcic I, et al. Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis. Cell. 2018;175:85-100.e23 pubmed publisher
Moore T, Wagner C, Scurti G, Hutchens K, Godellas C, Clark A, et al. Clinical and immunologic evaluation of three metastatic melanoma patients treated with autologous melanoma-reactive TCR-transduced T cells. Cancer Immunol Immunother. 2018;67:311-325 pubmed publisher
Ostadkarampour M, Müller M, Ockinger J, Kullberg S, Linden A, Eklund A, et al. Distinctive Regulatory T Cells and Altered Cytokine Profile Locally in the Airways of Young Smokers with Normal Lung Function. PLoS ONE. 2016;11:e0164751 pubmed publisher
Palamides P, Jodeleit H, Föhlinger M, Beigel F, Herbach N, Mueller T, et al. A mouse model for ulcerative colitis based on NOD-scid IL2R ?null mice reconstituted with peripheral blood mononuclear cells from affected individuals. Dis Model Mech. 2016;9:985-97 pubmed publisher
Groen B, van der Wijk A, van den Berg P, Lefrandt J, van den Berg G, Sollie K, et al. Immunological Adaptations to Pregnancy in Women with Type 1 Diabetes. Sci Rep. 2015;5:13618 pubmed publisher
Boyle M, Jagannathan P, Farrington L, Eccles James I, Wamala S, McIntyre T, et al. Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria. PLoS Pathog. 2015;11:e1005041 pubmed publisher
Chowdhury A, Hayes T, Bosinger S, Lawson B, Vanderford T, Schmitz J, et al. Differential Impact of In Vivo CD8+ T Lymphocyte Depletion in Controller versus Progressor Simian Immunodeficiency Virus-Infected Macaques. J Virol. 2015;89:8677-86 pubmed publisher
Tatovic D, Young P, Kochba E, Levin Y, Wong F, Dayan C. Fine-Needle Aspiration Biopsy of the Lymph Node: A Novel Tool for the Monitoring of Immune Responses after Skin Antigen Delivery. J Immunol. 2015;195:386-92 pubmed publisher
Chung Y, Kim E, Abdel Wahab O. Femoral bone marrow aspiration in live mice. J Vis Exp. 2014;: pubmed publisher
Xue J, Cong Z, Xiong J, Wang W, Jiang H, Chen T, et al. Repressive effect of primary virus replication on superinfection correlated with gut-derived central memory CD4(+) T cells in SHIV-infected Chinese rhesus macaques. PLoS ONE. 2013;8:e72295 pubmed publisher
Cicin Sain L, Smyk Pearson S, Smyk Paerson S, Currier N, Byrd L, Koudelka C, et al. Loss of naive T cells and repertoire constriction predict poor response to vaccination in old primates. J Immunol. 2010;184:6739-45 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
317450
Clone :
OKT4
Item :
APC/Cyanine7 anti-human CD4
Isotype :
Mouse IgG2b, κ
Other Names :
T4
Size :
100 μg
Price (USD) :
229 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
Human peripheral T cells
Application Notes :
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.^9;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA