product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 510™ anti-human CD4
catalog :
317443
quantity :
25 tests
price :
176 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV510
clone name :
OKT4
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 1b
Shen X, Geng R, Li Q, Chen Y, Li S, Wang Q, et al. ACE2-independent infection of T lymphocytes by SARS-CoV-2. Signal Transduct Target Ther. 2022;7:83 pubmed publisher
  • flow cytometry; human; loading ...; fig s12a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Inoue Y, Endo S, Matsuno N, Kikukawa Y, Shichijo T, Koga K, et al. Safety of mogamulizumab for relapsed ATL after allogeneic hematopoietic cell transplantation. Bone Marrow Transplant. 2018;: pubmed publisher
Sananez I, Raiden S, Algieri S, Uranga M, Grisolía N, Filippo D, et al. A poor and delayed anti-SARS-CoV2 IgG response is associated to severe COVID-19 in children. EBioMedicine. 2021;72:103615 pubmed publisher
Caduff N, McHugh D, Rieble L, Forconi C, Ong echa J, Oluoch P, et al. KSHV infection drives poorly cytotoxic CD56-negative natural killer cell differentiation in vivo upon KSHV/EBV dual infection. Cell Rep. 2021;35:109056 pubmed publisher
Vetsika E, Sharma P, Samaras I, Markou A, Georgoulias V, Whiteside T, et al. Small Extracellular Vesicles in Pre-Therapy Plasma Predict Clinical Outcome in Non-Small-Cell Lung Cancer Patients. Cancers (Basel). 2021;13: pubmed publisher
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
Wang S, Li M, Sun F, Chen C, Ye J, Li D, et al. Th17/Treg-cell balance in the peripheral blood of pregnant females with a history of recurrent spontaneous abortion receiving progesterone or cyclosporine A. Exp Ther Med. 2021;21:37 pubmed publisher
De Domenico E, Bonaguro L, Schulte Schrepping J, Becker M, Händler K, Schultze J. Optimized workflow for single-cell transcriptomics on infectious diseases including COVID-19. STAR Protoc. 2020;1:100233 pubmed publisher
Meckiff B, Ramírez Suástegui C, Fajardo V, Chee S, Kusnadi A, Simon H, et al. Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4+ T Cells in COVID-19. Cell. 2020;183:1340-1353.e16 pubmed publisher
Festag J, Thelemann T, Schell M, Raith S, Michel S, Jaschinski F, et al. Preventing ATP Degradation by ASO-Mediated Knockdown of CD39 and CD73 Results in A2aR-Independent Rescue of T Cell Proliferation. Mol Ther Nucleic Acids. 2020;21:656-669 pubmed publisher
Antonucci L, Porcu C, Timperi E, Santini S, Iannucci G, Balsano C. Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity. J Immunol Res. 2020;2020:4570219 pubmed publisher
Aguilar Briseño J, Upasani V, Ellen B, Moser J, Pauzuolis M, Ruiz Silva M, et al. TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis. Nat Commun. 2020;11:3177 pubmed publisher
Seydoux E, Homad L, MacCamy A, Parks K, Hurlburt N, Jennewein M, et al. Analysis of a SARS-CoV-2-Infected Individual Reveals Development of Potent Neutralizing Antibodies with Limited Somatic Mutation. Immunity. 2020;53:98-105.e5 pubmed publisher
Castellarin M, Sands C, Da T, Scholler J, Graham K, Buza E, et al. A rational mouse model to detect on-target, off-tumor CAR T cell toxicity. JCI Insight. 2020;5: pubmed publisher
Kalina T, Bakardjieva M, Blom M, Perez Andres M, Barendregt B, Kanderová V, et al. EuroFlow Standardized Approach to Diagnostic Immunopheneotyping of Severe PID in Newborns and Young Children. Front Immunol. 2020;11:371 pubmed publisher
Peng S, Zaretsky J, Ng A, Chour W, Bethune M, Choi J, et al. Sensitive Detection and Analysis of Neoantigen-Specific T Cell Populations from Tumors and Blood. Cell Rep. 2019;28:2728-2738.e7 pubmed publisher
van Dongen J, van der Burg M, Kalina T, Perez Andres M, Mejstrikova E, Vlkova M, et al. EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019;10:1271 pubmed publisher
Todnem Sakkestad S, Steinsland H, Skrede S, Kleppa E, Lillebø K, Sævik M, et al. Experimental Infection of Human Volunteers with the Heat-Stable Enterotoxin-Producing Enterotoxigenic Escherichia coli Strain TW11681. Pathogens. 2019;8: pubmed publisher
Herrera F, Valerio M, Berthold D, Tawadros T, Meuwly J, Vallet V, et al. 50-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial. Int J Radiat Oncol Biol Phys. 2019;103:320-334 pubmed publisher
Petrelli A, Mijnheer G, Hoytema van Konijnenburg D, van der Wal M, Giovannone B, Mocholí E, et al. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation. J Clin Invest. 2018;128:4669-4681 pubmed publisher
Heger L, Balk S, Lühr J, Heidkamp G, Lehmann C, Hatscher L, et al. CLEC10A Is a Specific Marker for Human CD1c+ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion. Front Immunol. 2018;9:744 pubmed publisher
Idorn M, Olsen M, Halldórsdóttir H, Skadborg S, Pedersen M, Høgdall C, et al. Improved migration of tumor ascites lymphocytes to ovarian cancer microenvironment by CXCR2 transduction. Oncoimmunology. 2018;7:e1412029 pubmed publisher
Wing A, Fajardo C, Posey A, Shaw C, Da T, Young R, et al. Improving CART-Cell Therapy of Solid Tumors with Oncolytic Virus-Driven Production of a Bispecific T-cell Engager. Cancer Immunol Res. 2018;6:605-616 pubmed publisher
Guedan S, Posey A, Shaw C, Wing A, Da T, Patel P, et al. Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation. JCI Insight. 2018;3: pubmed publisher
Lundtoft C, Afum Adjei Awuah A, Rimpler J, Harling K, Nausch N, Kohns M, et al. Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis. PLoS Pathog. 2017;13:e1006425 pubmed publisher
Mackroth M, Abel A, Steeg C, Schulze zur Wiesch J, Jacobs T. Acute Malaria Induces PD1+CTLA4+ Effector T Cells with Cell-Extrinsic Suppressor Function. PLoS Pathog. 2016;12:e1005909 pubmed publisher
Khaitan A, Kilberg M, Kravietz A, Ilmet T, Tastan C, Mwamzuka M, et al. HIV-Infected Children Have Lower Frequencies of CD8+ Mucosal-Associated Invariant T (MAIT) Cells that Correlate with Innate, Th17 and Th22 Cell Subsets. PLoS ONE. 2016;11:e0161786 pubmed publisher
Steindor M, Nkwouano V, Mayatepek E, Mackenzie C, Schramm D, Jacobsen M. Rapid detection and immune characterization of Mycobacterium abscessus infection in cystic fibrosis patients. PLoS ONE. 2015;10:e0119737 pubmed publisher
Willmann K, Klaver S, DoÄŸu F, Santos Valente E, Garncarz W, Bilic I, et al. Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity. Nat Commun. 2014;5:5360 pubmed publisher
Cicin Sain L, Smyk Pearson S, Smyk Paerson S, Currier N, Byrd L, Koudelka C, et al. Loss of naive T cells and repertoire constriction predict poor response to vaccination in old primates. J Immunol. 2010;184:6739-45 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
317443
Clone :
OKT4
Item :
Brilliant Violet 510™ anti-human CD4
Isotype :
Mouse IgG2b, κ
Other Names :
T4
Size :
25 tests
Price (USD) :
176 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV510
Immunogen :
Human peripheral T cells
Application Notes :
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.^9;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA