product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 650™ anti-human CD4
catalog :
317435
quantity :
25 tests
price :
220 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV650
clone name :
OKT4
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s8
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig s4e
Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
  • flow cytometry; human; fig 1
Frederiksen J, Buggert M, Noyan K, Nowak P, Sönnerborg A, Lund O, et al. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection. PLoS ONE. 2015;10:e0137635 pubmed publisher
  • flow cytometry; human
Misra R, Shah S, Fowell D, Wang H, Scheible K, Misra S, et al. Preterm cord blood CD4⁺ T cells exhibit increased IL-6 production in chorioamnionitis and decreased CD4⁺ T cells in bronchopulmonary dysplasia. Hum Immunol. 2015;76:329-338 pubmed publisher
  • flow cytometry; human
Buggert M, Tauriainen J, Yamamoto T, Frederiksen J, Ivarsson M, Michaelsson J, et al. T-bet and Eomes are differentially linked to the exhausted phenotype of CD8+ T cells in HIV infection. PLoS Pathog. 2014;10:e1004251 pubmed publisher
de Jonge K, Tillé L, Lourenço J, Maby El Hajjami H, Nassiri S, Racle J, et al. Inflammatory B cells correlate with failure to checkpoint blockade in melanoma patients. Oncoimmunology. 2021;10:1873585 pubmed publisher
Kim Y, Kobayashi E, Suehara Y, Ito A, Kubota D, Tanzawa Y, et al. Immunological status of peripheral blood is associated with prognosis in patients with bone and soft-tissue sarcoma. Oncol Lett. 2021;21:212 pubmed publisher
Rodda L, Netland J, Shehata L, Pruner K, Morawski P, Thouvenel C, et al. Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19. Cell. 2021;184:169-183.e17 pubmed publisher
Li D, Bradley T, Cain D, Pedroza Pacheco I, Aggelakopoulou M, Parks R, et al. RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures. Immunohorizons. 2020;4:713-728 pubmed publisher
Klemm F, Maas R, Bowman R, Kornete M, Soukup K, Nassiri S, et al. Interrogation of the Microenvironmental Landscape in Brain Tumors Reveals Disease-Specific Alterations of Immune Cells. Cell. 2020;181:1643-1660.e17 pubmed publisher
Huang B, Chen Z, Geng L, Wang J, Liang H, Cao Y, et al. Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways. Cell. 2019;179:1160-1176.e24 pubmed publisher
Wood M, Wood L, Templeton M, Fisher B, Lippy A, Jones C, et al. Transient Immune Activation in BCG-Vaccinated Infant Rhesus Macaques Is Not Sufficient to Influence Oral Simian Immunodeficiency Virus Infection. J Infect Dis. 2020;222:44-53 pubmed publisher
Del Alcazar D, Wang Y, He C, Wendel B, Del Rio Estrada P, Lin J, et al. Mapping the Lineage Relationship between CXCR5+ and CXCR5- CD4+ T Cells in HIV-Infected Human Lymph Nodes. Cell Rep. 2019;28:3047-3060.e7 pubmed publisher
Oll Hurtado M, Wolbert J, Fisher J, Flutter B, Stafford S, Barton J, et al. Tumor infiltrating lymphocytes expanded from pediatric neuroblastoma display heterogeneity of phenotype and function. PLoS ONE. 2019;14:e0216373 pubmed publisher
Cirelli K, Carnathan D, Nogal B, Martin J, Rodriguez O, Upadhyay A, et al. Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance. Cell. 2019;177:1153-1171.e28 pubmed publisher
Pauthner M, Nkolola J, Havenar Daughton C, Murrell B, Reiss S, Bastidas R, et al. Vaccine-Induced Protection from Homologous Tier 2 SHIV Challenge in Nonhuman Primates Depends on Serum-Neutralizing Antibody Titers. Immunity. 2019;50:241-252.e6 pubmed publisher
Neal J, Li X, Zhu J, Giangarrà V, Grzeskowiak C, Ju J, et al. Organoid Modeling of the Tumor Immune Microenvironment. Cell. 2018;175:1972-1988.e16 pubmed publisher
Jagannathan P, Lutwama F, Boyle M, Nankya F, Farrington L, McIntyre T, et al. Vδ2+ T cell response to malaria correlates with protection from infection but is attenuated with repeated exposure. Sci Rep. 2017;7:11487 pubmed publisher
Jacob V, Crawford C, Cohen Mekelburg S, Viladomiu M, Putzel G, Schneider Y, et al. Single Delivery of High-Diversity Fecal Microbiota Preparation by Colonoscopy Is Safe and Effective in Increasing Microbial Diversity in Active Ulcerative Colitis. Inflamm Bowel Dis. 2017;23:903-911 pubmed publisher
Loh L, Wang Z, Sant S, Koutsakos M, Jegaskanda S, Corbett A, et al. Human mucosal-associated invariant T cells contribute to antiviral influenza immunity via IL-18-dependent activation. Proc Natl Acad Sci U S A. 2016;113:10133-8 pubmed publisher
Dan J, Lindestam Arlehamn C, Weiskopf D, da Silva Antunes R, Havenar Daughton C, Reiss S, et al. A Cytokine-Independent Approach To Identify Antigen-Specific Human Germinal Center T Follicular Helper Cells and Rare Antigen-Specific CD4+ T Cells in Blood. J Immunol. 2016;197:983-93 pubmed publisher
Calascibetta F, Micci L, Carnathan D, Lawson B, Vanderford T, Bosinger S, et al. Antiretroviral Therapy in Simian Immunodeficiency Virus-Infected Sooty Mangabeys: Implications for AIDS Pathogenesis. J Virol. 2016;90:7541-7551 pubmed publisher
Kuebler P, Mehrotra M, McConnell J, Holditch S, Shaw B, Tarosso L, et al. Cellular immune correlates analysis of an HIV-1 preexposure prophylaxis trial. Proc Natl Acad Sci U S A. 2015;112:8379-84 pubmed publisher
Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed publisher
Mylvaganam G, Velu V, Hong J, Sadagopal S, Kwa S, Basu R, et al. Diminished viral control during simian immunodeficiency virus infection is associated with aberrant PD-1hi CD4 T cell enrichment in the lymphoid follicles of the rectal mucosa. J Immunol. 2014;193:4527-36 pubmed publisher
Cartwright E, McGary C, Cervasi B, Micci L, Lawson B, Elliott S, et al. Divergent CD4+ T memory stem cell dynamics in pathogenic and nonpathogenic simian immunodeficiency virus infections. J Immunol. 2014;192:4666-73 pubmed publisher
Buggert M, Frederiksen J, Noyan K, Svärd J, Barqasho B, Sonnerborg A, et al. Multiparametric bioinformatics distinguish the CD4/CD8 ratio as a suitable laboratory predictor of combined T cell pathogenesis in HIV infection. J Immunol. 2014;192:2099-108 pubmed publisher
Noto A, Ngauv P, Trautmann L. Cell-based flow cytometry assay to measure cytotoxic activity. J Vis Exp. 2013;:e51105 pubmed publisher
Cicin Sain L, Smyk Pearson S, Smyk Paerson S, Currier N, Byrd L, Koudelka C, et al. Loss of naive T cells and repertoire constriction predict poor response to vaccination in old primates. J Immunol. 2010;184:6739-45 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
317435
Clone :
OKT4
Item :
Brilliant Violet 650™ anti-human CD4
Isotype :
Mouse IgG2b, κ
Other Names :
T4
Size :
25 tests
Price (USD) :
220 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV650
Immunogen :
Human peripheral T cells
Application Notes :
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.^9;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA