product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human CD4
catalog :
317416
quantity :
100 tests
price :
72 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
OKT4
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 42
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:1000; loading ...
Zhang T, Xia W, Song X, Mao Q, Huang X, Chen B, et al. Super-enhancer hijacking LINC01977 promotes malignancy of early-stage lung adenocarcinoma addicted to the canonical TGF-β/SMAD3 pathway. J Hematol Oncol. 2022;15:114 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 1s3a
Bennstein S, Weinhold S, Manser A, Scherenschlich N, Noll A, Raba K, et al. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig s2
Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
  • flow cytometry; rhesus macaque
Frencher J, Shen H, Yan L, Wilson J, Freitag N, Rizzo A, et al. HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells. J Leukoc Biol. 2014;96:957-67 pubmed publisher
Mete B, Pekbilir E, Bilge B, Georgiadou P, Celik E, Sutlu T, et al. Human immunodeficiency virus type 1 impairs sumoylation. Life Sci Alliance. 2022;5: pubmed publisher
Zhu Z, Tang N, Wang M, Zhou J, Wang J, Ren H, et al. Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma. Front Immunol. 2021;12:781087 pubmed publisher
Beatson R, Parente Pereira A, Halim L, Cozzetto D, Hull C, Whilding L, et al. TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer. Cell Rep Med. 2021;2:100473 pubmed publisher
Wang Z, Li N, Feng K, Chen M, Zhang Y, Liu Y, et al. Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors. Cell Mol Immunol. 2021;18:2188-2198 pubmed publisher
Mishra A, Lai G, Yao L, Aung T, Shental N, Rotter Maskowitz A, et al. Microbial exposure during early human development primes fetal immune cells. Cell. 2021;184:3394-3409.e20 pubmed publisher
Dupont L, Bloor S, Williamson J, Cuesta S, Shah R, Teixeira Silva A, et al. The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr. Cell Host Microbe. 2021;29:792-805.e6 pubmed publisher
Soday L, Potts M, Hunter L, Ravenhill B, Houghton J, Williamson J, et al. Comparative Cell Surface Proteomic Analysis of the Primary Human T Cell and Monocyte Responses to Type I Interferon. Front Immunol. 2021;12:600056 pubmed publisher
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
Li N, Tang N, Cheng C, Hu T, Wei X, Han W, et al. Improving the anti-solid tumor efficacy of CAR-T cells by inhibiting adenosine signaling pathway. Oncoimmunology. 2020;9:1824643 pubmed publisher
Wang A, Yang M, Liang R, Zhu F, Zhu F, Liu X, et al. Mouse Double Minute 2 Homolog-Mediated Ubiquitination Facilitates Forkhead Box P3 Stability and Positively Modulates Human Regulatory T Cell Function. Front Immunol. 2020;11:1087 pubmed publisher
Saadati S, Eskandari V, Rahmani F, Mahmoudi M, Rahnemoon Z, Rahmati Z, et al. Gene Expression and Levels of TGF-B in PBMC Is Associated with Severity of Symptoms in Chronic Heart Failure. Avicenna J Med Biotechnol. 2020;12:132-134 pubmed
Arif S, Gomez Tourino I, Kamra Y, Pujol Autonell I, Hanton E, Tree T, et al. GAD-alum immunotherapy in type 1 diabetes expands bifunctional Th1/Th2 autoreactive CD4 T cells. Diabetologia. 2020;63:1186-1198 pubmed publisher
Kim K, Dauphin A, Kömürlü S, McCauley S, Yurkovetskiy L, Carbone C, et al. Cyclophilin A protects HIV-1 from restriction by human TRIM5α. Nat Microbiol. 2019;4:2044-2051 pubmed publisher
Claiborne D, Scully E, Palmer C, Prince J, Macharia G, Kopycinski J, et al. Protective HLA alleles are associated with reduced LPS levels in acute HIV infection with implications for immune activation and pathogenesis. PLoS Pathog. 2019;15:e1007981 pubmed publisher
Ahmed R, Omidian Z, Giwa A, Cornwell B, Majety N, Bell D, et al. A Public BCR Present in a Unique Dual-Receptor-Expressing Lymphocyte from Type 1 Diabetes Patients Encodes a Potent T Cell Autoantigen. Cell. 2019;177:1583-1599.e16 pubmed publisher
Cirelli K, Carnathan D, Nogal B, Martin J, Rodriguez O, Upadhyay A, et al. Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance. Cell. 2019;177:1153-1171.e28 pubmed publisher
Want M, Konstorum A, Huang R, Jain V, Matsueda S, Tsuji T, et al. Neoantigens retention in patient derived xenograft models mediates autologous T cells activation in ovarian cancer. Oncoimmunology. 2019;8:e1586042 pubmed publisher
Sueyoshi K, Ledderose C, Shen Y, Lee A, Shapiro N, Junger W. Lipopolysaccharide suppresses T cells by generating extracellular ATP that impairs their mitochondrial function via P2Y11 receptors. J Biol Chem. 2019;294:6283-6293 pubmed publisher
Azizi E, Carr A, Plitas G, Cornish A, Konopacki C, Prabhakaran S, et al. Single-Cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment. Cell. 2018;174:1293-1308.e36 pubmed publisher
Sun J, Liu T, Yan Y, Huo K, Zhang W, Liu H, et al. The role of Th1/Th2 cytokines played in regulation of specific CD4 + Th1 cell conversion and activation during inflammatory reaction of chronic obstructive pulmonary disease. Scand J Immunol. 2018;88:e12674 pubmed publisher
Kang H, Subramaniam M, Targ S, Nguyen M, Maliskova L, McCarthy E, et al. Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. Nat Biotechnol. 2017;: pubmed publisher
Dushyanthen S, Teo Z, Caramia F, Savas P, Mintoff C, Virassamy B, et al. Agonist immunotherapy restores T cell function following MEK inhibition improving efficacy in breast cancer. Nat Commun. 2017;8:606 pubmed publisher
Mujib S, Saiyed A, Fadel S, Bozorgzad A, Aidarus N, Yue F, et al. Pharmacologic HIV-1 Nef blockade promotes CD8 T cell-mediated elimination of latently HIV-1-infected cells in vitro. JCI Insight. 2017;2: pubmed publisher
Siedlik J, Deckert J, Benedict S, Bhatta A, Dunbar A, Vardiman J, et al. T cell activation and proliferation following acute exercise in human subjects is altered by storage conditions and mitogen selection. J Immunol Methods. 2017;446:7-14 pubmed publisher
Johnson A, Dirk B, Coutu M, Haeryfar S, Arts E, Finzi A, et al. A Highly Conserved Residue in HIV-1 Nef Alpha Helix 2 Modulates Protein Expression. mSphere. 2016;1: pubmed
Sugita S, Iwasaki Y, Makabe K, Kimura T, Futagami T, Suegami S, et al. Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. Stem Cell Reports. 2016;7:619-634 pubmed publisher
Narsale A, Moya R, Robertson H, Davies J. Data on correlations between T cell subset frequencies and length of partial remission in type 1 diabetes. Data Brief. 2016;8:1348-51 pubmed publisher
Comte D, Karampetsou M, Kis Toth K, Yoshida N, Bradley S, Mizui M, et al. Engagement of SLAMF3 enhances CD4+ T-cell sensitivity to IL-2 and favors regulatory T-cell polarization in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 2016;113:9321-6 pubmed publisher
Acerbi E, Viganò E, Poidinger M, Mortellaro A, Zelante T, Stella F. Continuous time Bayesian networks identify Prdm1 as a negative regulator of TH17 cell differentiation in humans. Sci Rep. 2016;6:23128 pubmed publisher
Bigler M, Egli S, Hysek C, Hoenger G, Schmied L, Baldin F, et al. Stress-Induced In Vivo Recruitment of Human Cytotoxic Natural Killer Cells Favors Subsets with Distinct Receptor Profiles and Associates with Increased Epinephrine Levels. PLoS ONE. 2015;10:e0145635 pubmed publisher
Claiborne D, Prince J, Scully E, Macharia G, Micci L, Lawson B, et al. Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression. Proc Natl Acad Sci U S A. 2015;112:E1480-9 pubmed publisher
Uhlenbrock F, Hagemann Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway. J Immunol. 2014;193:1654-65 pubmed publisher
Jafari M, Guatelli J, Lewinski M. Activities of transmitted/founder and chronic clade B HIV-1 Vpu and a C-terminal polymorphism specifically affecting virion release. J Virol. 2014;88:5062-78 pubmed publisher
Dotson A, Novikova L, Stehno Bittel L, Benedict S. Elimination of T cell reactivity to pancreatic ? cells and partial preservation of ? cell activity by peptide blockade of LFA-1:ICAM-1 interaction in the NOD mouse model. Clin Immunol. 2013;148:149-61 pubmed publisher
Sashihara J, Hoshino Y, Bowman J, Krogmann T, Burbelo P, Coffield V, et al. Soluble rhesus lymphocryptovirus gp350 protects against infection and reduces viral loads in animals that become infected with virus after challenge. PLoS Pathog. 2011;7:e1002308 pubmed publisher
Cicin Sain L, Smyk Pearson S, Smyk Paerson S, Currier N, Byrd L, Koudelka C, et al. Loss of naive T cells and repertoire constriction predict poor response to vaccination in old primates. J Immunol. 2010;184:6739-45 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Hayashi K, Altman A. Filamin A is required for T cell activation mediated by protein kinase C-theta. J Immunol. 2006;177:1721-8 pubmed
product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
317416
Clone :
OKT4
Item :
APC anti-human CD4
Isotype :
Mouse IgG2b, κ
Other Names :
T4
Size :
100 tests
Price (USD) :
72 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Immunogen :
Human peripheral T cells
Application Notes :
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.^9;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA