product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human CD4
catalog :
317408
quantity :
100 tests
price :
50 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
OKT4
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; fig 1a
Hsieh W, Hsu T, Chang Y, Lai M. IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells. Nat Commun. 2018;9:463 pubmed publisher
  • flow cytometry; human; fig s2e
Gee M, Han A, Lofgren S, Beausang J, Mendoza J, Birnbaum M, et al. Antigen Identification for Orphan T Cell Receptors Expressed on Tumor-Infiltrating Lymphocytes. Cell. 2018;172:549-563.e16 pubmed publisher
  • flow cytometry; human; loading ...; fig 6f
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling. Sci Rep. 2016;6:32320 pubmed publisher
  • flow cytometry; human
Clemente Casares X, Blanco J, Ambalavanan P, Yamanouchi J, Singha S, Fandos C, et al. Expanding antigen-specific regulatory networks to treat autoimmunity. Nature. 2016;530:434-40 pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Trabanelli S, Lecciso M, Salvestrini V, Cavo M, Očadlíková D, Lemoli R, et al. PGE2-induced IDO1 inhibits the capacity of fully mature DCs to elicit an in vitro antileukemic immune response. J Immunol Res. 2015;2015:253191 pubmed publisher
  • flow cytometry; human
Marin N, Paris S, Rojas M, Garcia L. Functional profile of CD4+ and CD8+ T cells in latently infected individuals and patients with active TB. Tuberculosis (Edinb). 2013;93:155-66 pubmed publisher
Teni Nurlatifah H, Barlianto W, Wiyasa W, Kusuma C, Sari T, Bachtiar N. Determination Factors Associated with Immune Responses Towards First Dose Edmonston-Zagreb (EZ) Measles Vaccine in Indonesian Infants. Med Arch. 2021;75:335-339 pubmed publisher
Rousso Noori L, Mastandrea I, Talmor S, Waks T, Globerson Levin A, Haugas M, et al. P32-specific CAR T cells with dual antitumor and antiangiogenic therapeutic potential in gliomas. Nat Commun. 2021;12:3615 pubmed publisher
Loo Yau H, De Carvalho D. Measuring the effect of drug treatments on primary human CD8+ T cell activation and cytolytic potential. STAR Protoc. 2021;2:100549 pubmed publisher
Pan Y, Aiamkitsumrit B, Bartolo L, Wang Y, Lavery C, Marc A, et al. Vaccination reshapes the virus-specific T cell repertoire in unexposed adults. Immunity. 2021;54:1245-1256.e5 pubmed publisher
Cai J, Gao H, Zhao J, Hu S, Liang X, Yang Y, et al. Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4+ T cells. elife. 2021;10: pubmed publisher
Glassman C, Mathiharan Y, Jude K, Su L, Panova O, Lupardus P, et al. Structural basis for IL-12 and IL-23 receptor sharing reveals a gateway for shaping actions on T versus NK cells. Cell. 2021;184:983-999.e24 pubmed publisher
Zhang Y, Dong X, Bai L, Shang X, Zeng Y. MUC1-induced immunosuppression in colon cancer can be reversed by blocking the PD1/PDL1 signaling pathway. Oncol Lett. 2020;20:317 pubmed publisher
Barresi V, Napoli S, Spampinato G, Condorelli D, Signorelli S. Dectin-1 and TIM3 Expression in Deep Vein Thrombosis of Lower Limbs (DVTLL). J Clin Med. 2020;9: pubmed publisher
Rodriguez Garcia A, Sharma P, Poussin M, Boesteanu A, Minutolo N, Gitto S, et al. CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies. Mol Ther. 2020;28:548-560 pubmed publisher
Del Alcazar D, Wang Y, He C, Wendel B, Del Rio Estrada P, Lin J, et al. Mapping the Lineage Relationship between CXCR5+ and CXCR5- CD4+ T Cells in HIV-Infected Human Lymph Nodes. Cell Rep. 2019;28:3047-3060.e7 pubmed publisher
Hixon J, Andrews C, Kashi L, Kohnhorst C, Senkevitch E, Czarra K, et al. New anti-IL-7Rα monoclonal antibodies show efficacy against T cell acute lymphoblastic leukemia in pre-clinical models. Leukemia. 2020;34:35-49 pubmed publisher
Yang M, Liu Y, Mo B, Xue Y, Ye C, Jiang Y, et al. Helios but not CD226, TIGIT and Foxp3 is a Potential Marker for CD4+ Treg Cells in Patients with Rheumatoid Arthritis. Cell Physiol Biochem. 2019;52:1178-1192 pubmed publisher
Varkey R, Du Q, Karnell J, Xiao X, Casey K, Woods R, et al. Discovery and characterization of potent IL-21 neutralizing antibodies via a novel alternating antigen immunization and humanization strategy. PLoS ONE. 2019;14:e0211236 pubmed publisher
Saraiva D, Jacinto A, Borralho P, Braga S, Cabral M. HLA-DR in Cytotoxic T Lymphocytes Predicts Breast Cancer Patients' Response to Neoadjuvant Chemotherapy. Front Immunol. 2018;9:2605 pubmed publisher
Buchan S, Dou L, Remer M, Booth S, Dunn S, Lai C, et al. Antibodies to Costimulatory Receptor 4-1BB Enhance Anti-tumor Immunity via T Regulatory Cell Depletion and Promotion of CD8 T Cell Effector Function. Immunity. 2018;49:958-970.e7 pubmed publisher
Meng S, Li L, Zhou M, Jiang W, Niu H, Yang K. Distribution and prognostic value of tumor‑infiltrating T cells in breast cancer. Mol Med Rep. 2018;18:4247-4258 pubmed publisher
Du Q, Wu X, Wang T, Yang X, Wang Z, Niu Y, et al. Porcine Circovirus Type 2 Suppresses IL-12p40 Induction via Capsid/gC1qR-Mediated MicroRNAs and Signalings. J Immunol. 2018;201:533-547 pubmed publisher
Wang L, Zhou D, Ren H, Chen Y. Effects of modified FOLFOX-6 chemotherapy on cellular immune function in patients with gastric cancer. Oncol Lett. 2018;15:8635-8640 pubmed publisher
Sasaki E, Momose H, Hiradate Y, Ishii K, Mizukami T, Hamaguchi I. In vitro marker gene expression analyses in human peripheral blood mononuclear cells: A tool to assess safety of influenza vaccines in humans. J Immunotoxicol. 2018;15:53-62 pubmed publisher
Zhou Y, Du J, Hou H, Lu Y, Yu J, Mao L, et al. Application of ImmunoScore Model for the Differentiation between Active Tuberculosis and Latent Tuberculosis Infection as Well as Monitoring Anti-tuberculosis Therapy. Front Cell Infect Microbiol. 2017;7:457 pubmed publisher
Hebbandi Nanjundappa R, Ronchi F, Wang J, Clemente Casares X, Yamanouchi J, Sokke Umeshappa C, et al. A Gut Microbial Mimic that Hijacks Diabetogenic Autoreactivity to Suppress Colitis. Cell. 2017;171:655-667.e17 pubmed publisher
Verma K, Jyotsana N, Buenting I, Luther S, Pfanne A, Thum T, et al. miR-625-3p is upregulated in CD8+ T cells during early immune reconstitution after allogeneic stem cell transplantation. PLoS ONE. 2017;12:e0183828 pubmed publisher
Kieffer T, Faas M, Scherjon S, Prins J. Pregnancy persistently affects memory T cell populations. J Reprod Immunol. 2017;119:1-8 pubmed publisher
Gorczynski R, Sadozai H, Zhu F, Khatri I. Effect of infusion of monoclonal antibodies to tumour necrosis factor-receptor super family 25 on graft rejection in allo-immune mice receiving autologous marrow transplantation. Immunology. 2017;150:418-431 pubmed publisher
Narsale A, Moya R, Robertson H, Davies J. Data on correlations between T cell subset frequencies and length of partial remission in type 1 diabetes. Data Brief. 2016;8:1348-51 pubmed publisher
Comte D, Karampetsou M, Kis Toth K, Yoshida N, Bradley S, Mizui M, et al. Engagement of SLAMF3 enhances CD4+ T-cell sensitivity to IL-2 and favors regulatory T-cell polarization in systemic lupus erythematosus. Proc Natl Acad Sci U S A. 2016;113:9321-6 pubmed publisher
Riaz T, Sollid L, Olsen I, de Souza G. Quantitative Proteomics of Gut-Derived Th1 and Th1/Th17 Clones Reveal the Presence of CD28+ NKG2D- Th1 Cytotoxic CD4+ T cells. Mol Cell Proteomics. 2016;15:1007-16 pubmed publisher
Bending D, Giannakopoulou E, Lom H, Wedderburn L. Synovial Regulatory T Cells Occupy a Discrete TCR Niche in Human Arthritis and Require Local Signals To Stabilize FOXP3 Protein Expression. J Immunol. 2015;195:5616-24 pubmed publisher
Yang B, Zhai F, Jiang J, Wang X, Cao Z, Cheng X. Elevated expression of T-bet in mycobacterial antigen-specific CD4(+) T cells from patients with tuberculosis. Cell Immunol. 2015;298:1-8 pubmed publisher
Enghard P, Rieder C, Kopetschke K, Klocke J, Undeutsch R, Biesen R, et al. Urinary CD4 T cells identify SLE patients with proliferative lupus nephritis and can be used to monitor treatment response. Ann Rheum Dis. 2014;73:277-83 pubmed publisher
Zhang T, Wu M, Sentman C. An NKp30-based chimeric antigen receptor promotes T cell effector functions and antitumor efficacy in vivo. J Immunol. 2012;189:2290-9 pubmed publisher
Cicin Sain L, Smyk Pearson S, Smyk Paerson S, Currier N, Byrd L, Koudelka C, et al. Loss of naive T cells and repertoire constriction predict poor response to vaccination in old primates. J Immunol. 2010;184:6739-45 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC
Cat # :
317408
Clone :
OKT4
Item :
FITC anti-human CD4
Isotype :
Mouse IgG2b, κ
Other Names :
T4
Size :
100 tests
Price (USD) :
50 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Immunogen :
Human peripheral T cells
Application Notes :
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.^9;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA