product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD4
catalog :
317402
quantity :
100 μg
price :
39 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
OKT4
reactivity :
human, mouse, rat, rhesus macaque
application :
immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 6a
Benkhoucha M, Tran N, Breville G, Senoner I, Bradfield P, Papayannopoulou T, et al. CD4+c-Met+Itgα4+ T cell subset promotes murine neuroinflammation. J Neuroinflammation. 2022;19:103 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 1b
Shen X, Geng R, Li Q, Chen Y, Li S, Wang Q, et al. ACE2-independent infection of T lymphocytes by SARS-CoV-2. Signal Transduct Target Ther. 2022;7:83 pubmed publisher
  • flow cytometry; human; fig 1b
Ming S, Yin H, Li X, Gong S, Zhang G, Wu Y. GITR Promotes the Polarization of TFH-Like Cells in Helicobacter pylori-Positive Gastritis. Front Immunol. 2021;12:736269 pubmed publisher
  • flow cytometry; human; 1:50; loading ...
Turner J, Kim W, Kalaidina E, Goss C, Rauseo A, Schmitz A, et al. SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans. Nature. 2021;595:421-425 pubmed publisher
  • flow cytometry; human
Hawerkamp H, Domdey A, Radau L, Sewerin P, Oláh P, Homey B, et al. Tofacitinib downregulates antiviral immune defence in keratinocytes and reduces T cell activation. Arthritis Res Ther. 2021;23:144 pubmed publisher
  • flow cytometry; human; loading ...
Martínez Zamudio R, Dewald H, Vasilopoulos T, Gittens Williams L, Fitzgerald Bocarsly P, Herbig U. Senescence-associated β-galactosidase reveals the abundance of senescent CD8+ T cells in aging humans. Aging Cell. 2021;20:e13344 pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • flow cytometry; human; 1:20; fig 5a
Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Chen H, Cong X, Wu C, Wu X, Wang J, Mao K, et al. Intratumoral delivery of CCL25 enhances immunotherapy against triple-negative breast cancer by recruiting CCR9+ T cells. Sci Adv. 2020;6:eaax4690 pubmed publisher
  • flow cytometry; human; loading ...; fig s12a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; human; loading ...; fig e1a, 3h
Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed publisher
  • flow cytometry; human; loading ...; fig 7c, 7f
Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster C, et al. PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease. Sci Rep. 2019;9:16941 pubmed publisher
  • immunocytochemistry; rat; 1:400; loading ...; fig 4a
Fletcher Jones A, Hildick K, Evans A, Nakamura Y, Wilkinson K, Henley J. The C-terminal helix 9 motif in rat cannabinoid receptor type 1 regulates axonal trafficking and surface expression. elife. 2019;8: pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
  • flow cytometry; human; loading ...; fig s8
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
  • flow cytometry; human; loading ...; fig s3c
Wiedemann G, Aithal C, Kraechan A, Heise C, Cadilha B, Zhang J, et al. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma. Transl Oncol. 2019;12:350-360 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 1c
Hoang T, Harper J, Pino M, Wang H, Micci L, King C, et al. Bone Marrow-Derived CD4+ T Cells Are Depleted in Simian Immunodeficiency Virus-Infected Macaques and Contribute to the Size of the Replication-Competent Reservoir. J Virol. 2019;93: pubmed publisher
  • flow cytometry; human; loading ...; fig 1e
Walwyn Brown K, Guldevall K, Saeed M, Pende D, Önfelt B, MacDonald A, et al. Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1. J Immunol. 2018;201:2028-2041 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Inoue Y, Endo S, Matsuno N, Kikukawa Y, Shichijo T, Koga K, et al. Safety of mogamulizumab for relapsed ATL after allogeneic hematopoietic cell transplantation. Bone Marrow Transplant. 2018;: pubmed publisher
  • flow cytometry; human; loading ...; fig 1
Ondigo B, Ndombi E, Nicholson S, Oguso J, Carter J, Kittur N, et al. Functional Studies of T Regulatory Lymphocytes in Human Schistosomiasis in Western Kenya. Am J Trop Med Hyg. 2018;98:1770-1781 pubmed publisher
  • flow cytometry; human; loading ...; fig s2
Clayton K, Collins D, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, et al. Resistance of HIV-infected macrophages to CD8+ T lymphocyte-mediated killing drives activation of the immune system. Nat Immunol. 2018;19:475-486 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Li M, Zhang W, Liu J, Li M, Zhang Y, Xiong Y, et al. Dynamic changes in the immunological characteristics of T lymphocytes in surviving patients with severe fever with thrombocytopenia syndrome (SFTS). Int J Infect Dis. 2018;70:72-80 pubmed publisher
  • flow cytometry; human; loading ...; fig 2d
Seki A, Rutz S. Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. J Exp Med. 2018;215:985-997 pubmed publisher
  • flow cytometry; human; loading ...; fig 5c
Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Matos T, O Malley J, Lowry E, Hamm D, Kirsch I, Robins H, et al. Clinically resolved psoriatic lesions contain psoriasis-specific IL-17-producing ?? T cell clones. J Clin Invest. 2017;127:4031-4041 pubmed publisher
  • flow cytometry; human; loading ...; fig 6a
Zhang J, Xu X, Shi M, Chen Y, Yu D, Zhao C, et al. CD13hi Neutrophil-like myeloid-derived suppressor cells exert immune suppression through Arginase 1 expression in pancreatic ductal adenocarcinoma. Oncoimmunology. 2017;6:e1258504 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig s4e
Mylvaganam G, Rios D, Abdelaal H, Iyer S, Tharp G, Mavigner M, et al. Dynamics of SIV-specific CXCR5+ CD8 T cells during chronic SIV infection. Proc Natl Acad Sci U S A. 2017;114:1976-1981 pubmed publisher
  • flow cytometry; human; loading ...
Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Ye C, Wang W, Cheng L, Li G, Wen M, Wang Q, et al. Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human; 1:25; loading ...; fig 2a
Siegers G, Barreira C, Postovit L, Dekaban G. CD11d ?2 integrin expression on human NK, B, and ?? T cells. J Leukoc Biol. 2017;101:1029-1035 pubmed publisher
  • flow cytometry; human; fig 4s1b
Puerta Arias J, Pino Tamayo P, Arango J, Gonzalez A. Depletion of Neutrophils Promotes the Resolution of Pulmonary Inflammation and Fibrosis in Mice Infected with Paracoccidioides brasiliensis. PLoS ONE. 2016;11:e0163985 pubmed publisher
  • flow cytometry; human; loading ...; fig 6f
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling. Sci Rep. 2016;6:32320 pubmed publisher
  • flow cytometry; human; fig s4a
Paquin Proulx D, Gibbs A, Bachle S, Checa A, Introini A, Leeansyah E, et al. Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion. J Immunol. 2016;197:1843-51 pubmed publisher
  • flow cytometry; human; loading ...; fig s3
Ruibal P, Oestereich L, Lüdtke A, Becker Ziaja B, Wozniak D, Kerber R, et al. Unique human immune signature of Ebola virus disease in Guinea. Nature. 2016;533:100-4 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 8a
Zurawski G, Zurawski S, Flamar A, Richert L, Wagner R, Tomaras G, et al. Targeting HIV-1 Env gp140 to LOX-1 Elicits Immune Responses in Rhesus Macaques. PLoS ONE. 2016;11:e0153484 pubmed publisher
  • flow cytometry; human; fig 1
Thibaudin M, Chaix M, Boidot R, Végran F, Derangère V, Limagne E, et al. Human ectonucleotidase-expressing CD25high Th17 cells accumulate in breast cancer tumors and exert immunosuppressive functions. Oncoimmunology. 2016;5:e1055444 pubmed
  • flow cytometry; rhesus macaque; fig 3
Bolton D, Pegu A, Wang K, McGinnis K, Nason M, Foulds K, et al. Human Immunodeficiency Virus Type 1 Monoclonal Antibodies Suppress Acute Simian-Human Immunodeficiency Virus Viremia and Limit Seeding of Cell-Associated Viral Reservoirs. J Virol. 2016;90:1321-32 pubmed publisher
  • flow cytometry; mouse
Akhmetzyanova I, Drabczyk M, Neff C, Gibbert K, Dietze K, Werner T, et al. PD-L1 Expression on Retrovirus-Infected Cells Mediates Immune Escape from CD8+ T Cell Killing. PLoS Pathog. 2015;11:e1005224 pubmed publisher
  • flow cytometry; human; fig 2
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
  • flow cytometry; human; 1:200; fig 1
Adoro S, Cubillos Ruiz J, Chen X, Deruaz M, Vrbanac V, Song M, et al. IL-21 induces antiviral microRNA-29 in CD4 T cells to limit HIV-1 infection. Nat Commun. 2015;6:7562 pubmed publisher
  • flow cytometry; rhesus macaque; fig 4
Chowdhury A, Hayes T, Bosinger S, Lawson B, Vanderford T, Schmitz J, et al. Differential Impact of In Vivo CD8+ T Lymphocyte Depletion in Controller versus Progressor Simian Immunodeficiency Virus-Infected Macaques. J Virol. 2015;89:8677-86 pubmed publisher
  • flow cytometry; human; fig 6
Anandasabapathy N, Breton G, Hurley A, Caskey M, Trumpfheller C, Sarma P, et al. Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers. Bone Marrow Transplant. 2015;50:924-30 pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Trabanelli S, Lecciso M, Salvestrini V, Cavo M, Očadlíková D, Lemoli R, et al. PGE2-induced IDO1 inhibits the capacity of fully mature DCs to elicit an in vitro antileukemic immune response. J Immunol Res. 2015;2015:253191 pubmed publisher
  • flow cytometry; human
Renauer P, Coit P, Sawalha A. The DNA methylation signature of human TCRαβ+CD4-CD8- double negative T cells reveals CG demethylation and a unique epigenetic architecture permissive to a broad stimulatory immune response. Clin Immunol. 2015;156:19-27 pubmed publisher
  • flow cytometry; human
Willmann K, Klaver S, DoÄŸu F, Santos Valente E, Garncarz W, Bilic I, et al. Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity. Nat Commun. 2014;5:5360 pubmed publisher
  • flow cytometry; human
Lee Chang C, Bodogai M, Moritoh K, Olkhanud P, Chan A, Croft M, et al. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity. Blood. 2014;124:1450-9 pubmed publisher
  • flow cytometry; rhesus macaque
Cartwright E, McGary C, Cervasi B, Micci L, Lawson B, Elliott S, et al. Divergent CD4+ T memory stem cell dynamics in pathogenic and nonpathogenic simian immunodeficiency virus infections. J Immunol. 2014;192:4666-73 pubmed publisher
Lou F, Sun Y, Xu Z, Niu L, Wang Z, Deng S, et al. Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. Immunity. 2020;53:204-216.e10 pubmed publisher
Bovay A, Speiser D, Fuertes Marraco S. Early drop of circulating T cells negatively correlates with the protective immune response to Yellow Fever vaccination. Hum Vaccin Immunother. 2020;16:3103-3110 pubmed publisher
Ghosh S, Di Bartolo V, Tubul L, Shimoni E, Kartvelishvily E, Dadosh T, et al. ERM-Dependent Assembly of T Cell Receptor Signaling and Co-stimulatory Molecules on Microvilli prior to Activation. Cell Rep. 2020;30:3434-3447.e6 pubmed publisher
Gernoux G, Gruntman A, Blackwood M, Zieger M, Flotte T, Mueller C. Muscle-Directed Delivery of an AAV1 Vector Leads to Capsid-Specific T Cell Exhaustion in Nonhuman Primates and Humans. Mol Ther. 2020;28:747-757 pubmed publisher
Oberg H, Janitschke L, Sulaj V, Weimer J, Gonnermann D, Hedemann N, et al. Bispecific antibodies enhance tumor-infiltrating T cell cytotoxicity against autologous HER-2-expressing high-grade ovarian tumors. J Leukoc Biol. 2020;107:1081-1095 pubmed publisher
Kong R, Duan H, Sheng Z, Xu K, Acharya P, Chen X, et al. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization. Cell. 2019;178:567-584.e19 pubmed publisher
Ardain A, Domingo Gonzalez R, Das S, Kazer S, Howard N, Singh A, et al. Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis. Nature. 2019;: pubmed publisher
Sueyoshi K, Ledderose C, Shen Y, Lee A, Shapiro N, Junger W. Lipopolysaccharide suppresses T cells by generating extracellular ATP that impairs their mitochondrial function via P2Y11 receptors. J Biol Chem. 2019;294:6283-6293 pubmed publisher
Li Y, Teteloshvili N, Tan S, Rao S, Han A, Yang Y, et al. Humanized Mice Reveal New Insights Into the Thymic Selection of Human Autoreactive CD8+ T Cells. Front Immunol. 2019;10:63 pubmed publisher
Wang B, Zuo J, Kang W, Wei Q, Li J, Wang C, et al. Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9. Mol Ther Nucleic Acids. 2018;11:130-141 pubmed publisher
Brinkmann C, Højen J, Rasmussen T, Kjær A, Olesen R, Denton P, et al. Treatment of HIV-Infected Individuals with the Histone Deacetylase Inhibitor Panobinostat Results in Increased Numbers of Regulatory T Cells and Limits Ex Vivo Lipopolysaccharide-Induced Inflammatory Responses. mSphere. 2018;3: pubmed publisher
Seyfarth J, Ahlert H, Rosenbauer J, Baechle C, Roden M, Holl R, et al. CISH promoter polymorphism effects on T cell cytokine receptor signaling and type 1 diabetes susceptibility. Mol Cell Pediatr. 2018;5:2 pubmed publisher
Prévost J, Richard J, Ding S, Pacheco B, Charlebois R, Hahn B, et al. Envelope glycoproteins sampling states 2/3 are susceptible to ADCC by sera from HIV-1-infected individuals. Virology. 2018;515:38-45 pubmed publisher
Majri S, Fritz J, Villarino A, Zheng L, Kanellopoulou C, Chaigne Delalande B, et al. STAT5B: A Differential Regulator of the Life and Death of CD4+ Effector Memory T Cells. J Immunol. 2018;200:110-118 pubmed publisher
Santos R, Bai L, Singh P, Murakami N, Fan H, Zhan W, et al. Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes. Nat Commun. 2017;8:1692 pubmed publisher
Ma F, Zhang Y, Xing J, Song X, Huang L, Weng H, et al. Fecal bacteria from treatment-naive Crohn's disease patients can skew helper T cell responses. Exp Cell Res. 2017;361:135-140 pubmed publisher
Horn L, Ciavattone N, Atkinson R, Woldergerima N, Wolf J, Clements V, et al. CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized mice. Oncotarget. 2017;8:57964-57980 pubmed publisher
Cardoso V, Chesne J, Ribeiro H, García Cassani B, Carvalho T, Bouchery T, et al. Neuronal regulation of type 2 innate lymphoid cells via neuromedin U. Nature. 2017;549:277-281 pubmed publisher
Martins M, Shin Y, Gonzalez Nieto L, Domingues A, Gutman M, Maxwell H, et al. Vaccine-induced immune responses against both Gag and Env improve control of simian immunodeficiency virus replication in rectally challenged rhesus macaques. PLoS Pathog. 2017;13:e1006529 pubmed publisher
Volchenkov R, Nygaard V, Şener Z, Skålhegg B. Th17 Polarization under Hypoxia Results in Increased IL-10 Production in a Pathogen-Independent Manner. Front Immunol. 2017;8:698 pubmed publisher
Alsahafi N, Richard J, Prévost J, Coutu M, Brassard N, Parsons M, et al. Impaired Downregulation of NKG2D Ligands by Nef Proteins from Elite Controllers Sensitizes HIV-1-Infected Cells to Antibody-Dependent Cellular Cytotoxicity. J Virol. 2017;91: pubmed publisher
Martins M, Tully D, Shin Y, Gonzalez Nieto L, Weisgrau K, Bean D, et al. Rare Control of SIVmac239 Infection in a Vaccinated Rhesus Macaque. AIDS Res Hum Retroviruses. 2017;33:843-858 pubmed publisher
Cicin Sain L, Smyk Pearson S, Smyk Paerson S, Currier N, Byrd L, Koudelka C, et al. Loss of naive T cells and repertoire constriction predict poor response to vaccination in old primates. J Immunol. 2010;184:6739-45 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
product information
Antigen :
CD4
Apps. Abbrev. :
FC, IHC-F
Cat # :
317402
Clone :
OKT4
Item :
Purified anti-human CD4
Isotype :
Mouse IgG2b, κ
Other Names :
T4
Size :
100 μg
Price (USD) :
39 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Human peripheral T cells
Application Notes :
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.^9;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA