product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-human CD3
catalog :
317349
quantity :
50 mg
price :
2354 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
OKT3
reactivity :
human, mouse
application :
flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments, other
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
Hickman T, Choi E, Whiteman K, Muralidharan S, Pai T, Johnson T, et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE. 2022;17:e0266980 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Wang Z, He L, Li W, Xu C, Zhang J, Wang D, et al. GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a, 5c
Harro C, Perez Sanz J, Costich T, Payne K, Anadon C, Chaurio R, et al. Methyltransferase inhibitors restore SATB1 protective activity against cutaneous T cell lymphoma in mice. J Clin Invest. 2021;131: pubmed publisher
  • other; mouse
Tseng H, Xiong W, Badeti S, Yang Y, Ma M, Liu T, et al. Efficacy of anti-CD147 chimeric antigen receptors targeting hepatocellular carcinoma. Nat Commun. 2020;11:4810 pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Fischer M, Ruhnau J, Schulze J, Obst D, Floel A, Vogelgesang A. Spermine and spermidine modulate T-cell function in older adults with and without cognitive decline ex vivo. Aging (Albany NY). 2020;12:13716-13739 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Pecher A, Kettemann F, Asteriti E, Schmid H, Duerr Stoerzer S, Keppeler H, et al. Invariant natural killer T cells are functionally impaired in patients with systemic sclerosis. Arthritis Res Ther. 2019;21:212 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml; loading ...; fig s10e
Lodygin D, Hermann M, Schweingruber N, Flügel Koch C, Watanabe T, Schlosser C, et al. β-Synuclein-reactive T cells induce autoimmune CNS grey matter degeneration. Nature. 2019;566:503-508 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Muller Durovic B, Grählert J, Devine O, Akbar A, Hess C. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age. Aging (Albany NY). 2019;11:724-740 pubmed publisher
  • blocking or activating experiments; human; 1 ug/ml; loading ...; fig 8f
Klement J, Paschall A, Redd P, Ibrahim M, Lu C, Yang D, et al. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. J Clin Invest. 2018;128:5549-5560 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 6d
Jung I, Kim Y, Yu H, Lee M, Kim S, Lee J. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res. 2018;78:4692-4703 pubmed publisher
Babin L, Darchen A, Robert E, Aid Z, Borry R, Soudais C, et al. De novo generation of the NPM-ALK fusion recapitulates the pleiotropic phenotypes of ALK+ ALCL pathogenesis and reveals the ROR2 receptor as target for tumor cells. Mol Cancer. 2022;21:65 pubmed publisher
Glassman C, Mathiharan Y, Jude K, Su L, Panova O, Lupardus P, et al. Structural basis for IL-12 and IL-23 receptor sharing reveals a gateway for shaping actions on T versus NK cells. Cell. 2021;184:983-999.e24 pubmed publisher
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
Saragovi A, Abramovich I, Omar I, Arbib E, Toker O, Gottlieb E, et al. Systemic hypoxia inhibits T cell response by limiting mitobiogenesis via matrix substrate-level phosphorylation arrest. elife. 2020;9: pubmed publisher
Hu B, Tang T, Jia J, Xie B, Wu T, Sheng Y, et al. CD137 agonist induces gastric cancer cell apoptosis by enhancing the functions of CD8+ T cells via NF-κB signaling. Cancer Cell Int. 2020;20:513 pubmed publisher
Sekine T, Perez Potti A, Rivera Ballesteros O, Stralin K, Gorin J, Olsson A, et al. Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19. Cell. 2020;183:158-168.e14 pubmed publisher
Campos Carrascosa L, van Beek A, de Ruiter V, Doukas M, Wei J, Fisher T, et al. FcγRIIB engagement drives agonistic activity of Fc-engineered αOX40 antibody to stimulate human tumor-infiltrating T cells. J Immunother Cancer. 2020;8: pubmed publisher
Pan W, Sharabi A, Ferretti A, Zhang Y, Burbano C, Yoshida N, et al. PPP2R2D suppresses IL-2 production and Treg function. JCI Insight. 2020;5: pubmed publisher
Si J, Shi X, Sun S, Zou B, Li Y, An D, et al. Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. Cancer Cell. 2020;38:551-566.e11 pubmed publisher
Evgin L, Huff A, Wongthida P, Thompson J, Kottke T, Tonne J, et al. Oncolytic virus-derived type I interferon restricts CAR T cell therapy. Nat Commun. 2020;11:3187 pubmed publisher
Correnti C, Hallinan J, Doyle L, Ruff R, Jaeger Ruckstuhl C, Xu Y, et al. Engineering and functionalization of large circular tandem repeat protein nanoparticles. Nat Struct Mol Biol. 2020;27:342-350 pubmed publisher
Mancuso R, Casper J, Schmidt A, Krahenbuhl S, Weitz Schmidt G. Anti-αLβ2 antibodies reveal novel endocytotic cross-modulatory functionality. Br J Pharmacol. 2020;177:2696-2711 pubmed publisher
Grandclaudon M, Perrot Dockès M, Trichot C, Karpf L, Abouzid O, Chauvin C, et al. A Quantitative Multivariate Model of Human Dendritic Cell-T Helper Cell Communication. Cell. 2019;179:432-447.e21 pubmed publisher
Sun T, Ju M, Dai X, Dong H, Gu W, Gao Y, et al. Multilevel defects in the hematopoietic niche in essential thrombocythemia. Haematologica. 2020;105:661-673 pubmed publisher
Wang J, RIEDER S, Wu J, Hayes S, Halpin R, de los Reyes M, et al. Evaluation of ultra-low input RNA sequencing for the study of human T cell transcriptome. Sci Rep. 2019;9:8445 pubmed publisher
Garris C, Arlauckas S, Kohler R, Trefny M, Garren S, Piot C, et al. Successful Anti-PD-1 Cancer Immunotherapy Requires T Cell-Dendritic Cell Crosstalk Involving the Cytokines IFN-γ and IL-12. Immunity. 2018;49:1148-1161.e7 pubmed publisher
Bak R, Dever D, Porteus M. CRISPR/Cas9 genome editing in human hematopoietic stem cells. Nat Protoc. 2018;13:358-376 pubmed publisher
Hu Y, Kim J, He K, Wan Q, Kim J, Flach M, et al. Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. J Exp Med. 2016;213:2759-2772 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
317349
Clone :
OKT3
Item :
Ultra-LEAF™ Purified anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
50 mg
Price (USD) :
2354 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
ULEAF
Application Notes :
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.^4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA