product summary
Loading...
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 700 anti-human CD3
catalog :
317340
quantity :
100 tests
price :
248 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF700
clone name :
OKT3
reactivity :
human, mouse
application :
flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments, other
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2b
Hickman T, Choi E, Whiteman K, Muralidharan S, Pai T, Johnson T, et al. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS ONE. 2022;17:e0266980 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Wang Z, He L, Li W, Xu C, Zhang J, Wang D, et al. GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a, 5c
Harro C, Perez Sanz J, Costich T, Payne K, Anadon C, Chaurio R, et al. Methyltransferase inhibitors restore SATB1 protective activity against cutaneous T cell lymphoma in mice. J Clin Invest. 2021;131: pubmed publisher
  • other; mouse
Tseng H, Xiong W, Badeti S, Yang Y, Ma M, Liu T, et al. Efficacy of anti-CD147 chimeric antigen receptors targeting hepatocellular carcinoma. Nat Commun. 2020;11:4810 pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Fischer M, Ruhnau J, Schulze J, Obst D, Floel A, Vogelgesang A. Spermine and spermidine modulate T-cell function in older adults with and without cognitive decline ex vivo. Aging (Albany NY). 2020;12:13716-13739 pubmed publisher
  • flow cytometry; human; loading ...; fig s2
Grifoni A, Weiskopf D, Ramirez S, Mateus J, Dan J, Moderbacher C, et al. Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals. Cell. 2020;181:1489-1501.e15 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Pecher A, Kettemann F, Asteriti E, Schmid H, Duerr Stoerzer S, Keppeler H, et al. Invariant natural killer T cells are functionally impaired in patients with systemic sclerosis. Arthritis Res Ther. 2019;21:212 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml; loading ...; fig s10e
Lodygin D, Hermann M, Schweingruber N, Flügel Koch C, Watanabe T, Schlosser C, et al. β-Synuclein-reactive T cells induce autoimmune CNS grey matter degeneration. Nature. 2019;566:503-508 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Muller Durovic B, Grählert J, Devine O, Akbar A, Hess C. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age. Aging (Albany NY). 2019;11:724-740 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s14a
Tan H, Jegaskanda S, Juno J, Esterbauer R, Wong J, Kelly H, et al. Subdominance and poor intrinsic immunogenicity limit humoral immunity targeting influenza HA stem. J Clin Invest. 2019;129:850-862 pubmed publisher
  • flow cytometry; human; loading ...; fig s3c
Wiedemann G, Aithal C, Kraechan A, Heise C, Cadilha B, Zhang J, et al. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma. Transl Oncol. 2019;12:350-360 pubmed publisher
  • blocking or activating experiments; human; 1 ug/ml; loading ...; fig 8f
Klement J, Paschall A, Redd P, Ibrahim M, Lu C, Yang D, et al. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. J Clin Invest. 2018;128:5549-5560 pubmed publisher
  • immunohistochemistry - paraffin section; human; loading ...; fig 6d
Jung I, Kim Y, Yu H, Lee M, Kim S, Lee J. CRISPR/Cas9-Mediated Knockout of DGK Improves Antitumor Activities of Human T Cells. Cancer Res. 2018;78:4692-4703 pubmed publisher
  • blocking or activating experiments; human; loading ...; fig 6a
Yang C, Chiu L, Chang C, Chuang H, Tan T. Induction of DUSP14 ubiquitination by PRMT5-mediated arginine methylation. FASEB J. 2018;:fj201800244RR pubmed publisher
  • flow cytometry; human; loading ...; fig s4a
Yeo L, Woodwyk A, Sood S, Lorenc A, Eichmann M, Pujol Autonell I, et al. Autoreactive T effector memory differentiation mirrors β cell function in type 1 diabetes. J Clin Invest. 2018;128:3460-3474 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Kiani Z, Dupuy F, Bruneau J, Lebouché B, Zhang C, Jackson E, et al. HLA-F on HLA-Null 721.221 Cells Activates Primary NK Cells Expressing the Activating Killer Ig-like Receptor KIR3DS1. J Immunol. 2018;201:113-123 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Provine N, Binder B, FitzPatrick M, Schuch A, Garner L, Williamson K, et al. Unique and Common Features of Innate-Like Human Vδ2+ γδT Cells and Mucosal-Associated Invariant T Cells. Front Immunol. 2018;9:756 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Jackson E, Zhang C, Kiani Z, Lisovsky I, Tallon B, Del Corpo A, et al. HIV exposed seronegative (HESN) compared to HIV infected individuals have higher frequencies of telomeric Killer Immunoglobulin-like Receptor (KIR) B motifs; Contribution of KIR B motif encoded genes to NK cell responsiveness. PLoS ONE. 2017;12:e0185160 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Aagaard K, Lahon A, Suter M, Arya R, Seferovic M, Vogt M, et al. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. Sci Rep. 2017;7:41389 pubmed publisher
  • flow cytometry; human; loading ...; fig s6a
Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
Tarke A, Sidney J, Kidd C, Dan J, Ramirez S, Yu E, et al. Comprehensive analysis of T cell immunodominance and immunoprevalence of SARS-CoV-2 epitopes in COVID-19 cases. Cell Rep Med. 2021;2:100204 pubmed publisher
Carbonneau S, Sharma S, Peng L, Rajan V, Hainzl D, Henault M, et al. An IMiD-inducible degron provides reversible regulation for chimeric antigen receptor expression and activity. Cell Chem Biol. 2021;28:802-812.e6 pubmed publisher
Apte S, Minigo G, Groves P, Spargo J, Plebanski M, Grigg M, et al. A population of CD4hiCD38hi T cells correlates with disease severity in patients with acute malaria. Clin Transl Immunology. 2020;9:e1209 pubmed publisher
Laing A, Lorenc A, Del Molino Del Barrio I, Das A, Fish M, Monin L, et al. A dynamic COVID-19 immune signature includes associations with poor prognosis. Nat Med. 2020;26:1623-1635 pubmed publisher
Grifoni A, Voic H, Dhanda S, Kidd C, Brien J, Buus S, et al. T Cell Responses Induced by Attenuated Flavivirus Vaccination Are Specific and Show Limited Cross-Reactivity with Other Flavivirus Species. J Virol. 2020;94: pubmed publisher
Tian Y, Seumois G, De Oliveira Pinto L, Mateus J, Herrera de la Mata S, Kim C, et al. Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease. Cell Rep. 2019;29:4482-4495.e4 pubmed publisher
Walk J, de Bree L, Graumans W, Stoter R, van Gemert G, van de Vegte Bolmer M, et al. Outcomes of controlled human malaria infection after BCG vaccination. Nat Commun. 2019;10:874 pubmed publisher
Dettling S, Stamova S, Warta R, Schnolzer M, Rapp C, Rathinasamy A, et al. Identification of CRKII, CFL1, CNTN1, NME2, and TKT as Novel and Frequent T-Cell Targets in Human IDH-Mutant Glioma. Clin Cancer Res. 2018;24:2951-2962 pubmed publisher
Biggs M, Milone M, Santos L, Gondarenko A, Wind S. High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. J R Soc Interface. 2011;8:1462-71 pubmed publisher
Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, et al. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30 pubmed publisher
Manevich Mendelson E, Feigelson S, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, et al. Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood. 2009;114:2344-53 pubmed publisher
Alter G, Rihn S, Streeck H, Teigen N, Piechocka Trocha A, Moss K, et al. Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection. J Virol. 2008;82:9668-77 pubmed publisher
Jeong H, Lee Y, Seo S, Lee S, Park S, Lee J, et al. Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection. J Leukoc Biol. 2008;83:755-64 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
317340
Clone :
OKT3
Item :
Alexa Fluor® 700 anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
248 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A700
Application Notes :
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.^4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA