product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 510™ anti-human CD3
catalog :
317332
quantity :
100 tests
price :
365 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV510
clone name :
OKT3
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...
Lu Q, Liu J, Zhao S, Gomez Castro M, Laurent Rolle M, Dong J, et al. SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2. Immunity. 2021;54:1304-1319.e9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s14a
Tan H, Jegaskanda S, Juno J, Esterbauer R, Wong J, Kelly H, et al. Subdominance and poor intrinsic immunogenicity limit humoral immunity targeting influenza HA stem. J Clin Invest. 2019;129:850-862 pubmed publisher
  • flow cytometry; human; fig s7
Di Marco Barros R, Roberts N, Dart R, Vantourout P, Jandke A, Nussbaumer O, et al. Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments. Cell. 2016;167:203-218.e17 pubmed publisher
  • flow cytometry; human; loading ...; fig s6a
Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed publisher
  • flow cytometry; human; fig 2
Leeansyah E, Svärd J, Dias J, Buggert M, Nyström J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
  • flow cytometry; human
Zhou J, Amran F, Kramski M, Angelovich T, Elliott J, Hearps A, et al. An NK Cell Population Lacking FcRγ Is Expanded in Chronically Infected HIV Patients. J Immunol. 2015;194:4688-97 pubmed publisher
Einkauf K, Osborn M, Gao C, Sun W, Sun X, Lian X, et al. Parallel analysis of transcription, integration, and sequence of single HIV-1 proviruses. Cell. 2022;185:266-282.e15 pubmed publisher
Motozono C, Toyoda M, Zahradník J, Saito A, Nasser H, Tan T, et al. SARS-CoV-2 spike L452R variant evades cellular immunity and increases infectivity. Cell Host Microbe. 2021;29:1124-1136.e11 pubmed publisher
Ehlers L, Kuppe A, Damerau A, Wilantri S, Kirchner M, Mertins P, et al. Surface AMP deaminase 2 as a novel regulator modifying extracellular adenine nucleotide metabolism. FASEB J. 2021;35:e21684 pubmed publisher
Kreutmair S, Unger S, Núñez N, Ingelfinger F, Alberti C, De Feo D, et al. Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia. Immunity. 2021;54:1578-1593.e5 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Piepenbrink M, Park J, Oladunni F, Deshpande A, Basu M, Sarkar S, et al. Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters. Cell Rep Med. 2021;2:100218 pubmed publisher
Sutton H, Aye R, Idris A, Vistein R, Nduati E, Kai O, et al. Atypical B cells are part of an alternative lineage of B cells that participates in responses to vaccination and infection in humans. Cell Rep. 2021;34:108684 pubmed publisher
Sant S, Quiñones Parra S, Koutsakos M, Grant E, Loudovaris T, Mannering S, et al. HLA-B*27:05 alters immunodominance hierarchy of universal influenza-specific CD8+ T cells. PLoS Pathog. 2020;16:e1008714 pubmed publisher
Antonucci L, Porcu C, Timperi E, Santini S, Iannucci G, Balsano C. Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity. J Immunol Res. 2020;2020:4570219 pubmed publisher
Juno J, Tan H, Lee W, Reynaldi A, Kelly H, Wragg K, et al. Humoral and circulating follicular helper T cell responses in recovered patients with COVID-19. Nat Med. 2020;26:1428-1434 pubmed publisher
Aguilar Briseño J, Upasani V, Ellen B, Moser J, Pauzuolis M, Ruiz Silva M, et al. TLR2 on blood monocytes senses dengue virus infection and its expression correlates with disease pathogenesis. Nat Commun. 2020;11:3177 pubmed publisher
Seydoux E, Homad L, MacCamy A, Parks K, Hurlburt N, Jennewein M, et al. Analysis of a SARS-CoV-2-Infected Individual Reveals Development of Potent Neutralizing Antibodies with Limited Somatic Mutation. Immunity. 2020;53:98-105.e5 pubmed publisher
Smalley M, Przedborski M, Thiyagarajan S, Pellowe M, Verma A, Brijwani N, et al. Integrating Systems Biology and an Ex Vivo Human Tumor Model Elucidates PD-1 Blockade Response Dynamics. iScience. 2020;23:101229 pubmed publisher
van den Hoogen P, de Jager S, Mol E, Schoneveld A, Huibers M, Vink A, et al. Potential of mesenchymal- and cardiac progenitor cells for therapeutic targeting of B-cells and antibody responses in end-stage heart failure. PLoS ONE. 2019;14:e0227283 pubmed publisher
Lamichhane R, Schneider M, de la Harpe S, Harrop T, Hannaway R, Dearden P, et al. TCR- or Cytokine-Activated CD8+ Mucosal-Associated Invariant T Cells Are Rapid Polyfunctional Effectors That Can Coordinate Immune Responses. Cell Rep. 2019;28:3061-3076.e5 pubmed publisher
Andrews S, Chambers M, Schramm C, Plyler J, Raab J, Kanekiyo M, et al. Activation Dynamics and Immunoglobulin Evolution of Pre-existing and Newly Generated Human Memory B cell Responses to Influenza Hemagglutinin. Immunity. 2019;51:398-410.e5 pubmed publisher
Cui J, Zhang Q, Song Q, Wang H, Dmitriev P, Sun M, et al. Targeting hypoxia downstream signaling protein, CAIX, for CAR T-cell therapy against glioblastoma. Neuro Oncol. 2019;21:1436-1446 pubmed publisher
Abd Hamid M, Wang R, Yao X, Fan P, Li X, Chang X, et al. Enriched HLA-E and CD94/NKG2A interaction limits antitumor CD8+ tumor-infiltrating T lymphocyte responses. Cancer Immunol Res. 2019;: pubmed publisher
Einkauf K, Lee G, Gao C, Sharaf R, Sun X, Hua S, et al. Intact HIV-1 proviruses accumulate at distinct chromosomal positions during prolonged antiretroviral therapy. J Clin Invest. 2019;129:988-998 pubmed publisher
Petrelli A, Mijnheer G, Hoytema van Konijnenburg D, van der Wal M, Giovannone B, Mocholí E, et al. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation. J Clin Invest. 2018;128:4669-4681 pubmed publisher
Jegaskanda S, Mason R, Andrews S, Wheatley A, Zhang R, Reynoso G, et al. Intranasal Live Influenza Vaccine Priming Elicits Localized B Cell Responses in Mediastinal Lymph Nodes. J Virol. 2018;92: pubmed publisher
Hearps A, Agius P, Zhou J, Brunt S, Chachage M, Angelovich T, et al. Persistence of Activated and Adaptive-Like NK Cells in HIV+ Individuals despite 2 Years of Suppressive Combination Antiretroviral Therapy. Front Immunol. 2017;8:731 pubmed publisher
Sordé L, Spindeldreher S, Palmer E, Karle A. Tregitopes and impaired antigen presentation: Drivers of the immunomodulatory effects of IVIg?. Immun Inflamm Dis. 2017;5:400-415 pubmed publisher
Belkina A, Snyder Cappione J. OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets. Cytometry A. 2017;91:175-179 pubmed publisher
Burian A, Wang K, Finton K, Lee N, Ishitani A, Strong R, et al. HLA-F and MHC-I Open Conformers Bind Natural Killer Cell Ig-Like Receptor KIR3DS1. PLoS ONE. 2016;11:e0163297 pubmed publisher
Wheatley A, Kristensen A, Lay W, Kent S. HIV-dependent depletion of influenza-specific memory B cells impacts B cell responsiveness to seasonal influenza immunisation. Sci Rep. 2016;6:26478 pubmed publisher
Gross C, Schulte Mecklenbeck A, Rünzi A, Kuhlmann T, Posevitz Fejfár A, Schwab N, et al. Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation. Proc Natl Acad Sci U S A. 2016;113:E2973-82 pubmed publisher
Ishizaka A, Sato H, Nakamura H, Koga M, Kikuchi T, Hosoya N, et al. Short Intracellular HIV-1 Transcripts as Biomarkers of Residual Immune Activation in Patients on Antiretroviral Therapy. J Virol. 2016;90:5665-5676 pubmed publisher
Chiu Y, Lin C, Sung B, Chuang Y, Schneck J, Kern F, et al. Cytotoxic polyfunctionality maturation of cytomegalovirus-pp65-specific CD4 + and CD8 + T-cell responses in older adults positively correlates with response size. Sci Rep. 2016;6:19227 pubmed publisher
Abreu C, Price S, Shirk E, Cunha R, Pianowski L, Clements J, et al. Dual role of novel ingenol derivatives from Euphorbia tirucalli in HIV replication: inhibition of de novo infection and activation of viral LTR. PLoS ONE. 2014;9:e97257 pubmed publisher
Biggs M, Milone M, Santos L, Gondarenko A, Wind S. High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. J R Soc Interface. 2011;8:1462-71 pubmed publisher
Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, et al. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30 pubmed publisher
Manevich Mendelson E, Feigelson S, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, et al. Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood. 2009;114:2344-53 pubmed publisher
Alter G, Rihn S, Streeck H, Teigen N, Piechocka Trocha A, Moss K, et al. Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection. J Virol. 2008;82:9668-77 pubmed publisher
Jeong H, Lee Y, Seo S, Lee S, Park S, Lee J, et al. Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection. J Leukoc Biol. 2008;83:755-64 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
317332
Clone :
OKT3
Item :
Brilliant Violet 510™ anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
365 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV510
Application Notes :
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.^4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA