product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 650™ anti-human CD3
catalog :
317324
quantity :
100 tests
price :
418 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV650
clone name :
OKT3
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2
Khosravi Maharlooei M, Li H, Hoelzl M, Zhao G, Ruiz A, Misra A, et al. Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice. J Autoimmun. 2021;119:102612 pubmed publisher
  • flow cytometry; human; loading ...; fig s2a
Rodda L, Netland J, Shehata L, Pruner K, Morawski P, Thouvenel C, et al. Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19. Cell. 2021;184:169-183.e17 pubmed publisher
  • flow cytometry; human; loading ...; fig s2o
Wirsching H, Zhang H, Szulzewsky F, Arora S, Grandi P, Cimino P, et al. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Wahid R, Fresnay S, Levine M, Sztein M. Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a. Clin Immunol. 2016;173:87-95 pubmed publisher
Khuzwayo S, Mthembu M, Meermeier E, Prakadan S, Kazer S, Bassett T, et al. MR1-Restricted MAIT Cells From The Human Lung Mucosal Surface Have Distinct Phenotypic, Functional, and Transcriptomic Features That Are Preserved in HIV Infection. Front Immunol. 2021;12:631410 pubmed publisher
Shuwa H, Shaw T, Knight S, Wemyss K, McClure F, Pearmain L, et al. Alterations in T and B cell function persist in convalescent COVID-19 patients. Med (N Y). 2021;2:720-735.e4 pubmed publisher
Clayton K, Mylvaganam G, Villasmil Ocando A, Stuart H, Maus M, Rashidian M, et al. HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses. Cell Host Microbe. 2021;29:435-447.e9 pubmed publisher
Pierce C, Preston Hurlburt P, Dai Y, Aschner C, Cheshenko N, Galen B, et al. Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients. Sci Transl Med. 2020;12: pubmed publisher
Ni J, Wang X, Stojanovic A, Zhang Q, Wincher M, Bühler L, et al. Single-Cell RNA Sequencing of Tumor-Infiltrating NK Cells Reveals that Inhibition of Transcription Factor HIF-1α Unleashes NK Cell Activity. Immunity. 2020;52:1075-1087.e8 pubmed publisher
Huang B, Chen Z, Geng L, Wang J, Liang H, Cao Y, et al. Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways. Cell. 2019;179:1160-1176.e24 pubmed publisher
Mikulak J, Oriolo F, Bruni E, Roberto A, Colombo F, Villa A, et al. NKp46-expressing human gut-resident intraepithelial Vδ1 T cell subpopulation exhibits high antitumor activity against colorectal cancer. JCI Insight. 2019;4: pubmed publisher
Brown C, Gudjonson H, Pritykin Y, Deep D, Lavallée V, Mendoza A, et al. Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity. Cell. 2019;179:846-863.e24 pubmed publisher
Escudero Pérez B, Ruibal P, Rottstegge M, Lüdtke A, Port J, Hartmann K, et al. Comparative pathogenesis of Ebola virus and Reston virus infection in humanized mice. JCI Insight. 2019;4: pubmed publisher
Guo J, Muse E, Christians A, Swanson S, Davila E. An Anticancer Drug Cocktail of Three Kinase Inhibitors Improved Response to a Dendritic Cell-Based Cancer Vaccine. Cancer Immunol Res. 2019;7:1523-1534 pubmed publisher
Booth J, Goldberg E, Patil S, Greenwald B, Sztein M. Association between S. Typhi-specific memory CD4+ and CD8+ T responses in the terminal ileum mucosa and in peripheral blood elicited by the live oral typhoid vaccine Ty21a in humans. Hum Vaccin Immunother. 2019;15:1409-1420 pubmed publisher
Franco L, Gadkari M, Howe K, Sun J, Kardava L, Kumar P, et al. Immune regulation by glucocorticoids can be linked to cell type-dependent transcriptional responses. J Exp Med. 2019;216:384-406 pubmed publisher
Savage T, Shonts B, Obradovic A, DeWolf S, Lau S, Zuber J, et al. Early expansion of donor-specific Tregs in tolerant kidney transplant recipients. JCI Insight. 2018;3: pubmed publisher
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
Querfeld C, Leung S, Myskowski P, Curran S, Goldman D, Heller G, et al. Primary T Cells from Cutaneous T-cell Lymphoma Skin Explants Display an Exhausted Immune Checkpoint Profile. Cancer Immunol Res. 2018;6:900-909 pubmed publisher
Waight J, Chand D, Dietrich S, Gombos R, Horn T, Gonzalez A, et al. Selective FcγR Co-engagement on APCs Modulates the Activity of Therapeutic Antibodies Targeting T Cell Antigens. Cancer Cell. 2018;33:1033-1047.e5 pubmed publisher
Garfall A, Stadtmauer E, Hwang W, Lacey S, Melenhorst J, Krevvata M, et al. Anti-CD19 CAR T cells with high-dose melphalan and autologous stem cell transplantation for refractory multiple myeloma. JCI Insight. 2018;3: pubmed publisher
Robert R, Juglair L, Lim E, Ang C, Wang C, Ebert G, et al. A fully humanized IgG-like bispecific antibody for effective dual targeting of CXCR3 and CCR6. PLoS ONE. 2017;12:e0184278 pubmed publisher
Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, et al. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1β, IFN-β, and IL-15 Pathways. J Immunol. 2015;195:2818-28 pubmed publisher
Boyle M, Jagannathan P, Farrington L, Eccles James I, Wamala S, McIntyre T, et al. Decline of FoxP3+ Regulatory CD4 T Cells in Peripheral Blood of Children Heavily Exposed to Malaria. PLoS Pathog. 2015;11:e1005041 pubmed publisher
Xu Y, Plazyo O, Romero R, Hassan S, Gomez Lopez N. Isolation of Leukocytes from the Human Maternal-fetal Interface. J Vis Exp. 2015;:e52863 pubmed publisher
Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
Breton G, Lee J, Zhou Y, Schreiber J, Keler T, PUHR S, et al. Circulating precursors of human CD1c+ and CD141+ dendritic cells. J Exp Med. 2015;212:401-13 pubmed publisher
Boyle M, Jagannathan P, Bowen K, McIntyre T, Vance H, Farrington L, et al. Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations. J Infect Dis. 2015;212:416-25 pubmed publisher
Riou C, Gray C, Lugongolo M, Gwala T, Kiravu A, Deniso P, et al. A subset of circulating blood mycobacteria-specific CD4 T cells can predict the time to Mycobacterium tuberculosis sputum culture conversion. PLoS ONE. 2014;9:e102178 pubmed publisher
Lucera M, Tilton C, Mao H, Dobrowolski C, Tabler C, Haqqani A, et al. The histone deacetylase inhibitor vorinostat (SAHA) increases the susceptibility of uninfected CD4+ T cells to HIV by increasing the kinetics and efficiency of postentry viral events. J Virol. 2014;88:10803-12 pubmed publisher
Boudreau J, Le Luduec J, Hsu K. Development of a novel multiplex PCR assay to detect functional subtypes of KIR3DL1 alleles. PLoS ONE. 2014;9:e99543 pubmed publisher
Tilton C, Tabler C, Lucera M, Marek S, Haqqani A, Tilton J. A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+ T cell subsets. J Virol Methods. 2014;195:164-9 pubmed publisher
Biggs M, Milone M, Santos L, Gondarenko A, Wind S. High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. J R Soc Interface. 2011;8:1462-71 pubmed publisher
Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, et al. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30 pubmed publisher
Manevich Mendelson E, Feigelson S, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, et al. Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood. 2009;114:2344-53 pubmed publisher
Alter G, Rihn S, Streeck H, Teigen N, Piechocka Trocha A, Moss K, et al. Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection. J Virol. 2008;82:9668-77 pubmed publisher
Jeong H, Lee Y, Seo S, Lee S, Park S, Lee J, et al. Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection. J Leukoc Biol. 2008;83:755-64 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
317324
Clone :
OKT3
Item :
Brilliant Violet 650™ anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
418 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV650
Application Notes :
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.^4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA