product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 605™ anti-human CD3
catalog :
317322
quantity :
100 tests
price :
424 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV605
clone name :
OKT3
reactivity :
human
application :
flow cytometry
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human
Ito S, Bollard C, Carlsten M, Melenhorst J, Biancotto A, Wang E, et al. Ultra-low dose interleukin-2 promotes immune-modulating function of regulatory T cells and natural killer cells in healthy volunteers. Mol Ther. 2014;22:1388-1395 pubmed publisher
Richert Spuhler L, MAR C, Shinde P, Wu F, Hong T, Greene E, et al. CD101 genetic variants modify regulatory and conventional T cell phenotypes and functions. Cell Rep Med. 2021;2:100322 pubmed publisher
Kalimuddin S, Tham C, Qui M, de Alwis R, Sim J, Lim J, et al. Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset. Med (N Y). 2021;2:682-688.e4 pubmed publisher
Shuwa H, Shaw T, Knight S, Wemyss K, McClure F, Pearmain L, et al. Alterations in T and B cell function persist in convalescent COVID-19 patients. Med (N Y). 2021;2:720-735.e4 pubmed publisher
Mengrelis K, Kucera F, Shahid N, Watt E, Ross S, Lau C, et al. T cell phenotype in paediatric heart transplant recipients. Pediatr Transplant. 2021;25:e13930 pubmed publisher
Wang J, Xu Y, Chen Z, Liang J, Lin Z, Liang H, et al. Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia. Cell. 2020;183:1867-1883.e26 pubmed publisher
Dyring Andersen B, Løvendorf M, Coscia F, Santos A, Møller L, Colaço A, et al. Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin. Nat Commun. 2020;11:5587 pubmed publisher
Martin Gayo E, Gao C, Chen H, Ouyang Z, Kim D, Kolb K, et al. Immunological Fingerprints of Controllers Developing Neutralizing HIV-1 Antibodies. Cell Rep. 2020;30:984-996.e4 pubmed publisher
Swadling L, Pallett L, Diniz M, Baker J, Amin O, Stegmann K, et al. Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence. Cell Rep. 2020;30:687-698.e6 pubmed publisher
Rodriguez Garcia A, Sharma P, Poussin M, Boesteanu A, Minutolo N, Gitto S, et al. CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies. Mol Ther. 2020;28:548-560 pubmed publisher
Huang B, Chen Z, Geng L, Wang J, Liang H, Cao Y, et al. Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways. Cell. 2019;179:1160-1176.e24 pubmed publisher
Brown C, Gudjonson H, Pritykin Y, Deep D, Lavallée V, Mendoza A, et al. Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity. Cell. 2019;179:846-863.e24 pubmed publisher
Leng T, Akther H, Hackstein C, Powell K, King T, Friedrich M, et al. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions. Cell Rep. 2019;28:3077-3091.e5 pubmed publisher
Guo J, Muse E, Christians A, Swanson S, Davila E. An Anticancer Drug Cocktail of Three Kinase Inhibitors Improved Response to a Dendritic Cell-Based Cancer Vaccine. Cancer Immunol Res. 2019;7:1523-1534 pubmed publisher
Zimmerman K, Bentley M, Lever J, Li Z, Crossman D, Song C, et al. Single-Cell RNA Sequencing Identifies Candidate Renal Resident Macrophage Gene Expression Signatures across Species. J Am Soc Nephrol. 2019;30:767-781 pubmed publisher
Zimmerman K, Gonzalez N, Chumley P, Chacana T, Harrington L, Yoder B, et al. Urinary T cells correlate with rate of renal function loss in autosomal dominant polycystic kidney disease. Physiol Rep. 2019;7:e13951 pubmed publisher
Kim M, Yu K, Kenderian S, Ruella M, Chen S, Shin T, et al. Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia. Cell. 2018;173:1439-1453.e19 pubmed publisher
Kuo H, Ahmad R, Lee G, Gao C, Chen H, Ouyang Z, et al. Anti-apoptotic Protein BIRC5 Maintains Survival of HIV-1-Infected CD4+ T Cells. Immunity. 2018;48:1183-1194.e5 pubmed publisher
Li J, Lee Y, Li Y, Jiang Y, Lu H, Zang W, et al. Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells. Immunity. 2018;48:773-786.e5 pubmed publisher
Watanabe K, Luo Y, Da T, Guedan S, Ruella M, Scholler J, et al. Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses. JCI Insight. 2018;3: pubmed publisher
Cao W, Kayama H, Chen M, Delmas A, Sun A, Kim S, et al. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids. Immunity. 2017;47:1182-1196.e10 pubmed publisher
Baxter A, Niessl J, Fromentin R, Richard J, Porichis F, Massanella M, et al. Multiparametric characterization of rare HIV-infected cells using an RNA-flow FISH technique. Nat Protoc. 2017;12:2029-2049 pubmed publisher
Day C, Abrahams D, Harris L, van Rooyen M, Stone L, de Kock M, et al. HIV-1 Infection Is Associated with Depletion and Functional Impairment of Mycobacterium tuberculosis-Specific CD4 T Cells in Individuals with Latent Tuberculosis Infection. J Immunol. 2017;199:2069-2080 pubmed publisher
Giudice V, Feng X, Kajigaya S, Young N, Biancotto A. Optimization and standardization of fluorescent cell barcoding for multiplexed flow cytometric phenotyping. Cytometry A. 2017;91:694-703 pubmed publisher
Stegmann K, Robertson F, Hansi N, Gill U, Pallant C, Christophides T, et al. CXCR6 marks a novel subset of T-bet(lo)Eomes(hi) natural killer cells residing in human liver. Sci Rep. 2016;6:26157 pubmed publisher
Biggs M, Milone M, Santos L, Gondarenko A, Wind S. High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. J R Soc Interface. 2011;8:1462-71 pubmed publisher
Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, et al. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30 pubmed publisher
Manevich Mendelson E, Feigelson S, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, et al. Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood. 2009;114:2344-53 pubmed publisher
Alter G, Rihn S, Streeck H, Teigen N, Piechocka Trocha A, Moss K, et al. Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection. J Virol. 2008;82:9668-77 pubmed publisher
Jeong H, Lee Y, Seo S, Lee S, Park S, Lee J, et al. Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection. J Leukoc Biol. 2008;83:755-64 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
317322
Clone :
OKT3
Item :
Brilliant Violet 605™ anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
100 tests
Price (USD) :
424 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV605
Application Notes :
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.^4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA