LEAF™ Purified anti-human CD3 | BioLegend 317315 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD3

catalog :

317315

quantity :

1 mg

price :

350 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

OKT3

reactivity :

human, mouse

application :

immunohistochemistry, flow cytometry, blocking or activating experiments

citations: 35

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig s4a	Yeo L, Woodwyk A, Sood S, Lorenc A, Eichmann M, Pujol Autonell I, et al. Autoreactive T effector memory differentiation mirrors β cell function in type 1 diabetes. J Clin Invest. 2018;128:3460-3474 pubmed publisher
flow cytometry; human; loading ...; fig 2a	Kiani Z, Dupuy F, Bruneau J, Lebouché B, Zhang C, Jackson E, et al. HLA-F on HLA-Null 721.221 Cells Activates Primary NK Cells Expressing the Activating Killer Ig-like Receptor KIR3DS1. J Immunol. 2018;201:113-123 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Provine N, Binder B, FitzPatrick M, Schuch A, Garner L, Williamson K, et al. Unique and Common Features of Innate-Like Human V?2+ ??T Cells and Mucosal-Associated Invariant T Cells. Front Immunol. 2018;9:756 pubmed publisher
flow cytometry; human; loading ...; fig 4a	Jackson E, Zhang C, Kiani Z, Lisovsky I, Tallon B, Del Corpo A, et al. HIV exposed seronegative (HESN) compared to HIV infected individuals have higher frequencies of telomeric Killer Immunoglobulin-like Receptor (KIR) B motifs; Contribution of KIR B motif encoded genes to NK cell responsiveness. PLoS ONE. 2017;12:e0185160 pubmed publisher
flow cytometry; human	Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, et al. IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition. Cancer Res. 2017;77:3268-3279 pubmed publisher
flow cytometry; human; loading ...; fig 3d	Jeffery H, Jeffery L, Lutz P, Corrigan M, Webb G, Hirschfield G, et al. Low-dose interleukin-2 promotes STAT-5 phosphorylation, Treg survival and CTLA-4-dependent function in autoimmune liver diseases. Clin Exp Immunol. 2017;188:394-411 pubmed publisher
flow cytometry; human; loading ...; fig s1a	Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed publisher
flow cytometry; human; loading ...; fig s3a	Aagaard K, Lahon A, Suter M, Arya R, Seferovic M, Vogt M, et al. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. Sci Rep. 2017;7:41389 pubmed publisher
blocking or activating experiments; human; fig 2a flow cytometry; human; fig 2a	Ribeiro S, Tesio M, Ribot J, Macintyre E, Barata J, Silva Santos B. Casein kinase 2 controls the survival of normal thymic and leukemic ?? T cells via promotion of AKT signaling. Leukemia. 2017;31:1603-1610 pubmed publisher
flow cytometry; human; loading ...; fig 4b	Ye C, Wang W, Cheng L, Li G, Wen M, Wang Q, et al. Glycosylphosphatidylinositol-Anchored Anti-HIV scFv Efficiently Protects CD4 T Cells from HIV-1 Infection and Deletion in hu-PBL Mice. J Virol. 2017;91: pubmed publisher
blocking or activating experiments; human; loading ...; fig s4a	Serr I, FÃ¼rst R, Ott V, Scherm M, Nikolaev A, GÃ¶kmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
flow cytometry; human; loading ...; fig s1	Isitman G, Tremblay McLean A, Lisovsky I, Bruneau J, Lebouché B, Routy J, et al. NK Cells Expressing the Inhibitory Killer Immunoglobulin-Like Receptors (iKIR) KIR2DL1, KIR2DL3 and KIR3DL1 Are Less Likely to Be CD16+ than Their iKIR Negative Counterparts. PLoS ONE. 2016;11:e0164517 pubmed publisher
blocking or activating experiments; human; loading ...	Jung Y, Riven I, Feigelson S, Kartvelishvily E, Tohya K, Miyasaka M, et al. Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies. Proc Natl Acad Sci U S A. 2016;113:E5916-E5924 pubmed
flow cytometry; human; loading ...; fig 5a	Wahid R, Fresnay S, Levine M, Sztein M. Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a. Clin Immunol. 2016;173:87-95 pubmed publisher
flow cytometry; human; loading ...; fig s6a	Landtwing V, Raykova A, Pezzino G, Beziat V, Marcenaro E, Graf C, et al. Cognate HLA absence in trans diminishes human NK cell education. J Clin Invest. 2016;126:3772-3782 pubmed publisher
flow cytometry; human; fig 2	Theorell J, Bryceson Y. Analysis of Intracellular Ca(2+) Mobilization in Human NK Cell Subsets by Flow Cytometry. Methods Mol Biol. 2016;1441:117-30 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
flow cytometry; human; fig 4	Cichocki F, Cooley S, Davis Z, DeFor T, Schlums H, Zhang B, et al. CD56dimCD57+NKG2C+ NK cell expansion is associated with reduced leukemia relapse after reduced intensity HCT. Leukemia. 2016;30:456-63 pubmed publisher
flow cytometry; mouse	Akhmetzyanova I, Drabczyk M, Neff C, Gibbert K, Dietze K, Werner T, et al. PD-L1 Expression on Retrovirus-Infected Cells Mediates Immune Escape from CD8+ T Cell Killing. PLoS Pathog. 2015;11:e1005224 pubmed publisher
flow cytometry; human; fig 2	Leeansyah E, SvÃ¤rd J, Dias J, Buggert M, NystrÃ¶m J, Quigley M, et al. Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection. PLoS Pathog. 2015;11:e1005072 pubmed publisher
flow cytometry; human; 1:200; fig s3	Adoro S, Cubillos Ruiz J, Chen X, Deruaz M, Vrbanac V, Song M, et al. IL-21 induces antiviral microRNA-29 in CD4 T cells to limit HIV-1 infection. Nat Commun. 2015;6:7562 pubmed publisher
flow cytometry; human; 1:200	Lee J, Breton G, Aljoufi A, Zhou Y, PUHR S, Nussenzweig M, et al. Clonal analysis of human dendritic cell progenitor using a stromal cell culture. J Immunol Methods. 2015;425:21-6 pubmed publisher
blocking or activating experiments; human; fig s7	Campia I, Buondonno I, Castella B, Rolando B, Kopecka J, Gazzano E, et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS ONE. 2015;10:e0126159 pubmed publisher
flow cytometry; human; fig 4	Lee J, Breton G, Oliveira T, Zhou Y, Aljoufi A, PUHR S, et al. Restricted dendritic cell and monocyte progenitors in human cord blood and bone marrow. J Exp Med. 2015;212:385-99 pubmed publisher
flow cytometry; human	Willmann K, Klaver S, DoÄŸu F, Santos Valente E, Garncarz W, Bilic I, et al. Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity. Nat Commun. 2014;5:5360 pubmed publisher
blocking or activating experiments; human	Boyd Tressler A, Penuela S, Laird D, Dubyak G. Chemotherapeutic drugs induce ATP release via caspase-gated pannexin-1 channels and a caspase/pannexin-1-independent mechanism. J Biol Chem. 2014;289:27246-63 pubmed publisher
flow cytometry; human	Prinz P, Mendler A, Brech D, Masouris I, Oberneder R, Noessner E. NK-cell dysfunction in human renal carcinoma reveals diacylglycerol kinase as key regulator and target for therapeutic intervention. Int J Cancer. 2014;135:1832-41 pubmed publisher
blocking or activating experiments; human	Bashour K, Gondarenko A, Chen H, Shen K, Liu X, Huse M, et al. CD28 and CD3 have complementary roles in T-cell traction forces. Proc Natl Acad Sci U S A. 2014;111:2241-6 pubmed publisher
	Ulmansky R, Landstein D, Moallem E, Loeb V, Levin A, Meyuhas R, et al. A Humanized Monoclonal Antibody against Heat Shock Protein 60 Suppresses Murine Arthritis and Colitis and Skews the Cytokine Balance toward an Anti-Inflammatory Response. J Immunol. 2015;194:5103-9 pubmed publisher
	Comrie W, Babich A, Burkhardt J. F-actin flow drives affinity maturation and spatial organization of LFA-1 at the immunological synapse. J Cell Biol. 2015;208:475-91 pubmed publisher
	Biggs M, Milone M, Santos L, Gondarenko A, Wind S. High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. J R Soc Interface. 2011;8:1462-71 pubmed publisher
	Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, et al. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30 pubmed publisher
	Manevich Mendelson E, Feigelson S, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, et al. Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood. 2009;114:2344-53 pubmed publisher
	Alter G, Rihn S, Streeck H, Teigen N, Piechocka Trocha A, Moss K, et al. Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection. J Virol. 2008;82:9668-77 pubmed publisher
	Jeong H, Lee Y, Seo S, Lee S, Park S, Lee J, et al. Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection. J Leukoc Biol. 2008;83:755-64 pubmed

product information

Antigen :

CD3

Apps. Abbrev. :

Flow Cytometry, IHC, Activ

Cat # :

317315

Clone :

OKT3

Item :

LEAF™ Purified anti-human CD3

Isotype :

Mouse IgG2a, κ

Other Names :

T3, CD3ε

Size :

1 mg

Price (USD) :

350 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. Clone OKT3 can block the binding of clones SK7 and UCHT1. 4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).