product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human CD3
catalog :
317305
quantity :
25 tests
price :
28 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
OKT3
reactivity :
human
application :
flow cytometry
more info or order :
citations: 34
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1a
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
  • flow cytometry; human; loading ...; fig s3a
Aagaard K, Lahon A, Suter M, Arya R, Seferovic M, Vogt M, et al. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. Sci Rep. 2017;7:41389 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
Akusjärvi S, Ambikan A, Krishnan S, Gupta S, Sperk M, Vegvari A, et al. Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling. iScience. 2022;25:103607 pubmed publisher
Cheng J, Myers T, Levinger C, Kumar P, Kumar J, Goshu B, et al. IL-27 induces IFN/STAT1-dependent genes and enhances function of TIGIT+ HIVGag-specific T cells. iScience. 2022;25:103588 pubmed publisher
Afolabi L, Bi J, Li X, Adeshakin A, Adeshakin F, Wu H, et al. Synergistic Tumor Cytolysis by NK Cells in Combination With a Pan-HDAC Inhibitor, Panobinostat. Front Immunol. 2021;12:701671 pubmed publisher
Loo Yau H, De Carvalho D. Measuring the effect of drug treatments on primary human CD8+ T cell activation and cytolytic potential. STAR Protoc. 2021;2:100549 pubmed publisher
Golebski K, Layhadi J, Sahiner U, Steveling Klein E, Lenormand M, Li R, et al. Induction of IL-10-producing type 2 innate lymphoid cells by allergen immunotherapy is associated with clinical response. Immunity. 2021;54:291-307.e7 pubmed publisher
Shi Z, Qiu Y, Zhao Z, Wen D, Chen H, Du Q, et al. CD8+ T cell subpopulations and pro-inflammatory cytokines in neuromyelitis optica spectrum disorder. Ann Clin Transl Neurol. 2021;8:43-53 pubmed publisher
Dai Q, Han P, Qi X, Li F, Li M, Fan L, et al. 4-1BB Signaling Boosts the Anti-Tumor Activity of CD28-Incorporated 2nd Generation Chimeric Antigen Receptor-Modified T Cells. Front Immunol. 2020;11:539654 pubmed publisher
Miyamae Y, Chen L, Utsugi Y, Farrants H, Wandless T. A Method for Conditional Regulation of Protein Stability in Native or Near-Native Form. Cell Chem Biol. 2020;27:1573-1581.e3 pubmed publisher
James K, Gomes T, Elmentaite R, Kumar N, Gulliver E, King H, et al. Distinct microbial and immune niches of the human colon. Nat Immunol. 2020;21:343-353 pubmed publisher
Hazenberg M, Haverkate N, van Lier Y, Spits H, Krabbendam L, Bemelman W, et al. Human ectoenzyme-expressing ILC3: immunosuppressive innate cells that are depleted in graft-versus-host disease. Blood Adv. 2019;3:3650-3660 pubmed publisher
Kim K, Dauphin A, Kömürlü S, McCauley S, Yurkovetskiy L, Carbone C, et al. Cyclophilin A protects HIV-1 from restriction by human TRIM5α. Nat Microbiol. 2019;4:2044-2051 pubmed publisher
Catapano M, Vergnano M, Romano M, Mahil S, Choon S, Burden A, et al. IL-36 Promotes Systemic IFN-I Responses in Severe Forms of Psoriasis. J Invest Dermatol. 2020;140:816-826.e3 pubmed publisher
Want M, Konstorum A, Huang R, Jain V, Matsueda S, Tsuji T, et al. Neoantigens retention in patient derived xenograft models mediates autologous T cells activation in ovarian cancer. Oncoimmunology. 2019;8:e1586042 pubmed publisher
Wang Y, Chen C, Qi J, Wu F, Guan J, Chen Z, et al. Altered PGE2-EP2 is associated with an excessive immune response in HBV-related acute-on-chronic liver failure. J Transl Med. 2019;17:93 pubmed publisher
Lin L, Li L, Zhou C, Li J, Liu J, Shu R, et al. A HER2 bispecific antibody can be efficiently expressed in Escherichia coli with potent cytotoxicity. Oncol Lett. 2018;16:1259-1266 pubmed publisher
Agashe V, Jankowska Gan E, Keller M, Sullivan J, Haynes L, Kernien J, et al. Leukocyte-Associated Ig-like Receptor 1 Inhibits Th1 Responses but Is Required for Natural and Induced Monocyte-Dependent Th17 Responses. J Immunol. 2018;201:772-781 pubmed publisher
Gillissen M, Kedde M, de Jong G, Moiset G, Yasuda E, Levie S, et al. AML-specific cytotoxic antibodies in patients with durable graft versus leukemia responses. Blood. 2017;: pubmed publisher
Giesecke C, Feher K, von Volkmann K, Kirsch J, Radbruch A, Kaiser T. Determination of background, signal-to-noise, and dynamic range of a flow cytometer: A novel practical method for instrument characterization and standardization. Cytometry A. 2017;91:1104-1114 pubmed publisher
Zitti B, Molfetta R, Fionda C, Quatrini L, Stabile H, Lecce M, et al. Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells. Sci Rep. 2017;7:10445 pubmed publisher
Shikhagaie M, Björklund Ã, Mjösberg J, Erjefält J, Cornelissen A, Ros X, et al. Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates. Cell Rep. 2017;18:1761-1773 pubmed publisher
Sullivan K, Lewis H, Hill A, Pandey A, Jackson L, Cabral J, et al. Trisomy 21 consistently activates the interferon response. elife. 2016;5: pubmed publisher
Guo X, Dash P, CALVERLEY M, Tomchuck S, Dallas M, Thomas P. Rapid cloning, expression, and functional characterization of paired αβ and γδ T-cell receptor chains from single-cell analysis. Mol Ther Methods Clin Dev. 2016;3:15054 pubmed publisher
Zaldivar Fujigaki J, Arroyo Valerio A, Lopez Alvarenga J, Gutiérrez Reyes E, Kershenobich D, Hernández Ruiz J. Alterations in Activation, Cytotoxic Capacity and Trafficking Profile of Peripheral CD8 T Cells in Young Adult Binge Drinkers. PLoS ONE. 2015;10:e0132521 pubmed publisher
Clayton K, Haaland M, Douglas Vail M, Mujib S, Chew G, Ndhlovu L, et al. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol. 2014;192:782-91 pubmed publisher
Zhao B, Zheng S, Gong L, Wang Y, Chen C, Jin W, et al. T lymphocytes from chronic HCV-infected patients are primed for activation-induced apoptosis and express unique pro-apoptotic gene signature. PLoS ONE. 2013;8:e77008 pubmed publisher
Ochi T, Fujiwara H, Okamoto S, An J, Nagai K, Shirakata T, et al. Novel adoptive T-cell immunotherapy using a WT1-specific TCR vector encoding silencers for endogenous TCRs shows marked antileukemia reactivity and safety. Blood. 2011;118:1495-503 pubmed publisher
Biggs M, Milone M, Santos L, Gondarenko A, Wind S. High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates. J R Soc Interface. 2011;8:1462-71 pubmed publisher
Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, et al. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30 pubmed publisher
Manevich Mendelson E, Feigelson S, Pasvolsky R, Aker M, Grabovsky V, Shulman Z, et al. Loss of Kindlin-3 in LAD-III eliminates LFA-1 but not VLA-4 adhesiveness developed under shear flow conditions. Blood. 2009;114:2344-53 pubmed publisher
Alter G, Rihn S, Streeck H, Teigen N, Piechocka Trocha A, Moss K, et al. Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection. J Virol. 2008;82:9668-77 pubmed publisher
Jeong H, Lee Y, Seo S, Lee S, Park S, Lee J, et al. Blocking of monocyte-associated B7-H1 (CD274) enhances HCV-specific T cell immunity in chronic hepatitis C infection. J Leukoc Biol. 2008;83:755-64 pubmed
product information
Antigen :
CD3
Apps. Abbrev. :
FC
Cat # :
317305
Clone :
OKT3
Item :
FITC anti-human CD3
Isotype :
Mouse IgG2a, κ
Other Names :
T3, CD3ε
Size :
25 tests
Price (USD) :
28 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Application Notes :
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.^4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA