product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human IgM (Maxpar® Ready)
catalog :
314527
quantity :
100 μg
price :
129 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MHM-88
reactivity :
African green monkey, human
application :
flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 26
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2a
Brouwer P, Caniels T, van der Straten K, Snitselaar J, Aldon Y, Bangaru S, et al. Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability. Science. 2020;369:643-650 pubmed publisher
  • mass cytometry; human; 500 ug/ml; loading ...; fig s11a
Helmink B, Reddy S, Gao J, Zhang S, Basar R, Thakur R, et al. B cells and tertiary lymphoid structures promote immunotherapy response. Nature. 2020;577:549-555 pubmed publisher
  • flow cytometry; human; loading ...; fig s6d
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
  • flow cytometry; human; loading ...; fig s2a
Dam E, Maier A, Hocking A, Carlin J, Buckner J. Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis. Front Immunol. 2018;9:1978 pubmed publisher
  • flow cytometry; human; loading ...; fig 5
O CONNOR D, Clutterbuck E, Thompson A, Snape M, Ramasamy M, Kelly D, et al. High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. Genome Med. 2017;9:11 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Wentink M, Dalm V, Lankester A, van Schouwenburg P, Schölvinck L, Kalina T, et al. Genetic defects in PI3K? affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections. Clin Immunol. 2017;176:77-86 pubmed publisher
  • blocking or activating experiments; human; fig 9b
Jackson S, Jacobs H, Arkatkar T, Dam E, Scharping N, Kolhatkar N, et al. B cell IFN-γ receptor signaling promotes autoimmune germinal centers via cell-intrinsic induction of BCL-6. J Exp Med. 2016;213:733-50 pubmed publisher
  • flow cytometry; African green monkey
Kwun J, Page E, Hong J, GIBBY A, Yoon J, Farris A, et al. Neutralizing BAFF/APRIL with atacicept prevents early DSA formation and AMR development in T cell depletion induced nonhuman primate AMR model. Am J Transplant. 2015;15:815-22 pubmed publisher
Stensland Z, Coleman B, Rihanek M, Baxter R, Gottlieb P, Hsieh E, et al. Peripheral immunophenotyping of AITD subjects reveals alterations in immune cells in pediatric vs adult-onset AITD. iScience. 2022;25:103626 pubmed publisher
Jung Y, Wen L, Altman A, Ley K. CD45 pre-exclusion from the tips of T cell microvilli prior to antigen recognition. Nat Commun. 2021;12:3872 pubmed publisher
Brouwer P, Brinkkemper M, Maisonnasse P, Dereuddre Bosquet N, Grobben M, Claireaux M, et al. Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection. Cell. 2021;184:1188-1200.e19 pubmed publisher
Dogan M, Kozhaya L, Placek L, Gunter C, Yigit M, Hardy R, et al. SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus. Commun Biol. 2021;4:129 pubmed publisher
Young Glazer J, Cisneros A, Wilfong E, Smith S, Crofford L, Bonami R. Jo-1 autoantigen-specific B cells are skewed towards distinct functional B cell subsets in anti-synthetase syndrome patients. Arthritis Res Ther. 2021;23:33 pubmed publisher
Gruber C, Patel R, Trachtman R, Lepow L, Amanat F, Krammer F, et al. Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell. 2020;183:982-995.e14 pubmed publisher
Gang E, Kim H, Hsieh Y, Ruan Y, Ogana H, Lee S, et al. Integrin α6 mediates the drug resistance of acute lymphoblastic B-cell leukemia. Blood. 2020;136:210-223 pubmed publisher
Stras S, Werner L, Toothaker J, Olaloye O, Oldham A, McCourt C, et al. Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development. Dev Cell. 2019;51:357-373.e5 pubmed publisher
Shehata L, Maurer D, Wec A, Lilov A, Champney E, Sun T, et al. Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability. Cell Rep. 2019;28:3300-3308.e4 pubmed publisher
Franco L, Gadkari M, Howe K, Sun J, Kardava L, Kumar P, et al. Immune regulation by glucocorticoids can be linked to cell type-dependent transcriptional responses. J Exp Med. 2019;216:384-406 pubmed publisher
van Montfoort N, Borst L, Korrer M, Sluijter M, Marijt K, Santegoets S, et al. NKG2A Blockade Potentiates CD8 T Cell Immunity Induced by Cancer Vaccines. Cell. 2018;175:1744-1755.e15 pubmed publisher
Baglaenko Y, Chang N, Johnson S, Hafiz W, Manion K, Ferri D, et al. The presence of anti-nuclear antibodies alone is associated with changes in B cell activation and T follicular helper cells similar to those in systemic autoimmune rheumatic disease. Arthritis Res Ther. 2018;20:264 pubmed publisher
Phelan J, Young R, Webster D, Roulland S, Wright G, Kasbekar M, et al. A multiprotein supercomplex controlling oncogenic signalling in lymphoma. Nature. 2018;560:387-391 pubmed publisher
Laksono B, Grosserichter Wagener C, de Vries R, Langeveld S, Brem M, van Dongen J, et al. In Vitro Measles Virus Infection of Human Lymphocyte Subsets Demonstrates High Susceptibility and Permissiveness of both Naive and Memory B Cells. J Virol. 2018;92: pubmed publisher
Blum L, Cao R, Sweatt A, Bill M, Lahey L, Hsi A, et al. Circulating plasmablasts are elevated and produce pathogenic anti-endothelial cell autoantibodies in idiopathic pulmonary arterial hypertension. Eur J Immunol. 2018;48:874-884 pubmed publisher
Pinder C, Kratochvil S, Cizmeci D, Muir L, Guo Y, Shattock R, et al. Isolation and Characterization of Antigen-Specific Plasmablasts Using a Novel Flow Cytometry-Based Ig Capture Assay. J Immunol. 2017;199:4180-4188 pubmed publisher
Arkatkar T, Du S, Jacobs H, Dam E, Hou B, Buckner J, et al. B cell-derived IL-6 initiates spontaneous germinal center formation during systemic autoimmunity. J Exp Med. 2017;214:3207-3217 pubmed publisher
Perez Shibayama C, Gil Cruz C, Pastelin Palacios R, Cervantes Barragan L, Hisaki E, Chai Q, et al. IFN-?-producing CD4+ T cells promote generation of protective germinal center-derived IgM+ B cell memory against Salmonella Typhi. J Immunol. 2014;192:5192-200 pubmed publisher
product information
Antigen :
IgM
Apps. Abbrev. :
FC, CyTOF®
Cat # :
314527
Clone :
MHM-88
Item :
Purified anti-human IgM (Maxpar® Ready)
Isotype :
Mouse IgG1, κ
Other Names :
Immunoglobulin M
Size :
100 μg
Price (USD) :
129 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PUREMAX
Immunogen :
Human Ig cocktail
Application Notes :
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA