product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human IgM
catalog :
314502
quantity :
100 μg
price :
101 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MHM-88
reactivity :
African green monkey, human
application :
ELISA, flow cytometry, blocking or activating experiments, mass cytometry
more info or order :
citations: 40
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2a
Brouwer P, Caniels T, van der Straten K, Snitselaar J, Aldon Y, Bangaru S, et al. Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability. Science. 2020;369:643-650 pubmed publisher
  • mass cytometry; human; 500 ug/ml; loading ...; fig s11a
Helmink B, Reddy S, Gao J, Zhang S, Basar R, Thakur R, et al. B cells and tertiary lymphoid structures promote immunotherapy response. Nature. 2020;577:549-555 pubmed publisher
  • flow cytometry; human; loading ...; fig s6d
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed publisher
  • flow cytometry; human; loading ...; fig s2a
Dam E, Maier A, Hocking A, Carlin J, Buckner J. Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis. Front Immunol. 2018;9:1978 pubmed publisher
  • flow cytometry; human; loading ...; fig 5
O CONNOR D, Clutterbuck E, Thompson A, Snape M, Ramasamy M, Kelly D, et al. High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. Genome Med. 2017;9:11 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Wentink M, Dalm V, Lankester A, van Schouwenburg P, Schölvinck L, Kalina T, et al. Genetic defects in PI3K? affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections. Clin Immunol. 2017;176:77-86 pubmed publisher
  • blocking or activating experiments; human; fig 9b
Jackson S, Jacobs H, Arkatkar T, Dam E, Scharping N, Kolhatkar N, et al. B cell IFN-γ receptor signaling promotes autoimmune germinal centers via cell-intrinsic induction of BCL-6. J Exp Med. 2016;213:733-50 pubmed publisher
  • flow cytometry; African green monkey
Kwun J, Page E, Hong J, GIBBY A, Yoon J, Farris A, et al. Neutralizing BAFF/APRIL with atacicept prevents early DSA formation and AMR development in T cell depletion induced nonhuman primate AMR model. Am J Transplant. 2015;15:815-22 pubmed publisher
Strother C, Brewer Jensen P, BECKER DREPS S, Zepeda O, May S, Gonzalez F, et al. Infant antibody and B-cell responses following confirmed pediatric GII.17 norovirus infections functionally distinguish GII.17 genetic clusters. Front Immunol. 2023;14:1229724 pubmed publisher
Siris S, Gladstone C, Guo Y, Patel R, Pinder C, Shattock R, et al. Increasing human monoclonal antibody cloning efficiency with a whole-cell modified immunoglobulin-capture assay (mICA). Front Immunol. 2023;14:1184510 pubmed publisher
Diray Arce J, Fourati S, Doni Jayavelu N, Patel R, Maguire C, Chang A, et al. Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patients. Cell Rep Med. 2023;4:101079 pubmed publisher
He W, Musharrafieh R, Song G, Dueker K, Tse L, Martinez D, et al. Targeted isolation of diverse human protective broadly neutralizing antibodies against SARS-like viruses. Nat Immunol. 2022;23:960-970 pubmed publisher
Pearson J, Ding H, Hu C, Peng J, Galuppo B, Wong F, et al. IgM-associated gut bacteria in obesity and type 2 diabetes in C57BL/6 mice and humans. Diabetologia. 2022;65:1398-1411 pubmed publisher
McCarthy E, Odorizzi P, Lutz E, Smullin C, Tenvooren I, Stone M, et al. A cytotoxic-skewed immune set point predicts low neutralizing antibody levels after Zika virus infection. Cell Rep. 2022;39:110815 pubmed publisher
Byazrova M, Kulemzin S, Astakhova E, Belovezhets T, Efimov G, Chikaev A, et al. Memory B Cells Induced by Sputnik V Vaccination Produce SARS-CoV-2 Neutralizing Antibodies Upon Ex Vivo Restimulation. Front Immunol. 2022;13:840707 pubmed publisher
He W, Musharrafieh R, Song G, Dueker K, Tse L, Martinez D, et al. Targeted isolation of panels of diverse human protective broadly neutralizing antibodies against SARS-like viruses. bioRxiv. 2022;: pubmed publisher
Georg P, Astaburuaga García R, Bonaguro L, Brumhard S, Michalick L, Lippert L, et al. Complement activation induces excessive T cell cytotoxicity in severe COVID-19. Cell. 2022;185:493-512.e25 pubmed publisher
Jung Y, Wen L, Altman A, Ley K. CD45 pre-exclusion from the tips of T cell microvilli prior to antigen recognition. Nat Commun. 2021;12:3872 pubmed publisher
Brouwer P, Brinkkemper M, Maisonnasse P, Dereuddre Bosquet N, Grobben M, Claireaux M, et al. Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infection. Cell. 2021;184:1188-1200.e19 pubmed publisher
Kennedy Darling J, Bhate S, Hickey J, Black S, Barlow G, Vazquez G, et al. Highly multiplexed tissue imaging using repeated oligonucleotide exchange reaction. Eur J Immunol. 2021;51:1262-1277 pubmed publisher
Dogan M, Kozhaya L, Placek L, Gunter C, Yigit M, Hardy R, et al. SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus. Commun Biol. 2021;4:129 pubmed publisher
Newell K, Clemmer D, Cox J, Kayode Y, Zoccoli Rodriguez V, Taylor H, et al. Switched and unswitched memory B cells detected during SARS-CoV-2 convalescence correlate with limited symptom duration. PLoS ONE. 2021;16:e0244855 pubmed publisher
Young Glazer J, Cisneros A, Wilfong E, Smith S, Crofford L, Bonami R. Jo-1 autoantigen-specific B cells are skewed towards distinct functional B cell subsets in anti-synthetase syndrome patients. Arthritis Res Ther. 2021;23:33 pubmed publisher
Mann E, Menon M, Knight S, Konkel J, Jagger C, Shaw T, et al. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19. Sci Immunol. 2020;5: pubmed publisher
Pyfrom S, Quinn C, Dorando H, Luo H, Payton J. BCALM (AC099524.1) Is a Human B Lymphocyte-Specific Long Noncoding RNA That Modulates B Cell Receptor-Mediated Calcium Signaling. J Immunol. 2020;205:595-607 pubmed publisher
Gang E, Kim H, Hsieh Y, Ruan Y, Ogana H, Lee S, et al. Integrin α6 mediates the drug resistance of acute lymphoblastic B-cell leukemia. Blood. 2020;136:210-223 pubmed publisher
Martin E, Minet N, Boschat A, Sanquer S, Sobrino S, Lenoir C, et al. Impaired lymphocyte function and differentiation in CTPS1-deficient patients result from a hypomorphic homozygous mutation. JCI Insight. 2020;5: pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
Waugh K, Araya P, Pandey A, Jordan K, Smith K, Granrath R, et al. Mass Cytometry Reveals Global Immune Remodeling with Multi-lineage Hypersensitivity to Type I Interferon in Down Syndrome. Cell Rep. 2019;29:1893-1908.e4 pubmed publisher
Shehata L, Maurer D, Wec A, Lilov A, Champney E, Sun T, et al. Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability. Cell Rep. 2019;28:3300-3308.e4 pubmed publisher
Mclaurin J, Weiner O. Multiple sources of signal amplification within the B-cell Ras/MAPK pathway. Mol Biol Cell. 2019;30:1610-1620 pubmed publisher
Franco L, Gadkari M, Howe K, Sun J, Kardava L, Kumar P, et al. Immune regulation by glucocorticoids can be linked to cell type-dependent transcriptional responses. J Exp Med. 2019;216:384-406 pubmed publisher
Baglaenko Y, Chang N, Johnson S, Hafiz W, Manion K, Ferri D, et al. The presence of anti-nuclear antibodies alone is associated with changes in B cell activation and T follicular helper cells similar to those in systemic autoimmune rheumatic disease. Arthritis Res Ther. 2018;20:264 pubmed publisher
Phelan J, Young R, Webster D, Roulland S, Wright G, Kasbekar M, et al. A multiprotein supercomplex controlling oncogenic signalling in lymphoma. Nature. 2018;560:387-391 pubmed publisher
Laksono B, Grosserichter Wagener C, de Vries R, Langeveld S, Brem M, van Dongen J, et al. In Vitro Measles Virus Infection of Human Lymphocyte Subsets Demonstrates High Susceptibility and Permissiveness of both Naive and Memory B Cells. J Virol. 2018;92: pubmed publisher
Blum L, Cao R, Sweatt A, Bill M, Lahey L, Hsi A, et al. Circulating plasmablasts are elevated and produce pathogenic anti-endothelial cell autoantibodies in idiopathic pulmonary arterial hypertension. Eur J Immunol. 2018;48:874-884 pubmed publisher
Pinder C, Kratochvil S, Cizmeci D, Muir L, Guo Y, Shattock R, et al. Isolation and Characterization of Antigen-Specific Plasmablasts Using a Novel Flow Cytometry-Based Ig Capture Assay. J Immunol. 2017;199:4180-4188 pubmed publisher
Arkatkar T, Du S, Jacobs H, Dam E, Hou B, Buckner J, et al. B cell-derived IL-6 initiates spontaneous germinal center formation during systemic autoimmunity. J Exp Med. 2017;214:3207-3217 pubmed publisher
Kolhatkar N, Brahmandam A, Thouvenel C, Becker Herman S, Jacobs H, Schwartz M, et al. Altered BCR and TLR signals promote enhanced positive selection of autoreactive transitional B cells in Wiskott-Aldrich syndrome. J Exp Med. 2015;212:1663-77 pubmed publisher
Perez Shibayama C, Gil Cruz C, Pastelin Palacios R, Cervantes Barragan L, Hisaki E, Chai Q, et al. IFN-?-producing CD4+ T cells promote generation of protective germinal center-derived IgM+ B cell memory against Salmonella Typhi. J Immunol. 2014;192:5192-200 pubmed publisher
product information
Antigen :
IgM
Apps. Abbrev. :
FC, ELISA
Cat # :
314502
Clone :
MHM-88
Item :
Purified anti-human IgM
Isotype :
Mouse IgG1, κ
Other Names :
Immunoglobulin M
Size :
100 μg
Price (USD) :
101 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Human Ig cocktail
Application Notes :
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA