product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human/mouse CD49f
catalog :
313602
quantity :
100 μg
price :
119 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
GoH3
reactivity :
human, mouse, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, other, mass cytometry
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; loading ...; fig 4a
van der Heide V, Jangra S, Cohen P, Rathnasinghe R, Aslam S, Aydillo T, et al. Limited extent and consequences of pancreatic SARS-CoV-2 infection. Cell Rep. 2022;38:110508 pubmed publisher
  • flow cytometry; mouse
Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 7d
Tsutsui K, Machida H, Nakagawa A, Ahn K, Morita R, Sekiguchi K, et al. Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions. Nat Commun. 2021;12:2577 pubmed publisher
  • flow cytometry; mouse; 1:200
Mevel R, Steiner I, Mason S, Galbraith L, Patel R, Fadlullah M, et al. RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:2000; loading ...; fig s1-1a
Tremblay M, Viala S, Shafer M, Graham Paquin A, Liu C, Bouchard M. Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation. elife. 2020;9: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...
Xi L, Carroll T, Matos I, Luo J, Polak L, Pasolli H, et al. m6A RNA methylation impacts fate choices during skin morphogenesis. elife. 2020;9: pubmed publisher
  • other; human; 1:100; loading ...
Leelatian N, Sinnaeve J, Mistry A, Barone S, Brockman A, Diggins K, et al. Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5c, 7s4b
Engelbrecht E, Lévesque M, He L, Vanlandewijck M, Nitzsche A, Niazi H, et al. Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta. elife. 2020;9: pubmed publisher
  • flow cytometry; human; 1:20; loading ...; fig 2d
  • flow cytometry; mouse; 1:100; loading ...; fig 2s2c
Katsuda T, Matsuzaki J, Yamaguchi T, Yamada Y, Prieto Vila M, Hosaka K, et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. elife. 2019;8: pubmed publisher
  • flow cytometry; human; loading ...; fig 9b
  • flow cytometry; mouse; loading ...; fig 2a
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3c
Bagchi S, He Y, Zhang H, Cao L, Van Rhijn I, Moody D, et al. CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice. J Clin Invest. 2017;127:2339-2352 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Zhao C, Cai S, Shin K, Lim A, Kalisky T, Lu W, et al. Stromal Gli2 activity coordinates a niche signaling program for mammary epithelial stem cells. Science. 2017;356: pubmed publisher
  • flow cytometry; human; 1:25; loading ...; fig 3a
Britschgi A, Duss S, Kim S, Couto J, Brinkhaus H, Koren S, et al. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα. Nature. 2017;541:541-545 pubmed publisher
  • flow cytometry; mouse; fig 2a
Yokota Nakatsuma A, Ohoka Y, Takeuchi H, Song S, Iwata M. Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties. Sci Rep. 2016;6:37914 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
  • other; human; loading ...; fig st1
Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  • immunohistochemistry; mouse; fig s1
Adachi T, Kobayashi T, Sugihara E, Yamada T, Ikuta K, Pittaluga S, et al. Hair follicle-derived IL-7 and IL-15 mediate skin-resident memory T cell homeostasis and lymphoma. Nat Med. 2015;21:1272-9 pubmed publisher
  • western blot; human; 1:1000
Virts E, Jankowska A, MacKay C, Glaas M, Wiek C, Kelich S, et al. AluY-mediated germline deletion, duplication and somatic stem cell reversion in UBE2T defines a new subtype of Fanconi anemia. Hum Mol Genet. 2015;24:5093-108 pubmed publisher
  • flow cytometry; human; fig 1
Hines W, Yaswen P, Bissell M. Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias. Nat Commun. 2015;6:6927 pubmed publisher
  • flow cytometry; human; fig 5
Termini C, Cotter M, Marjon K, Buranda T, Lidke K, Gillette J. The membrane scaffold CD82 regulates cell adhesion by altering α4 integrin stability and molecular density. Mol Biol Cell. 2014;25:1560-73 pubmed publisher
  • flow cytometry; mouse
Goldstein A, Huang J, Guo C, Garraway I, Witte O. Identification of a cell of origin for human prostate cancer. Science. 2010;329:568-71 pubmed publisher
Zahavi D, Erbe R, Zhang Y, Guo T, Malchiodi Z, Maynard R, et al. Antibody dependent cell-mediated cytotoxicity selection pressure induces diverse mechanisms of resistance. Cancer Biol Ther. 2023;24:2269637 pubmed publisher
Sekihara K, Himuro H, Saito N, Ota Y, Kouro T, Kusano Y, et al. Evaluation of X-ray and carbon-ion beam irradiation with chemotherapy for the treatment of cervical adenocarcinoma cells in 2D and 3D cultures. Cancer Cell Int. 2022;22:391 pubmed publisher
Ishibashi R, Maki R, Kitano S, Miyachi H, Toyoshima F. Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci Rep. 2022;12:17775 pubmed publisher
Shannon J, Kirchner S, Zhang J. Human Skin Explant Preparation and Culture. Bio Protoc. 2022;12: pubmed publisher
Doffe F, Bonini F, Lakis E, Terry S, Chouaib S, Savagner P. Designing Organoid Models to Monitor Cancer Progression, Plasticity and Resistance: The Right Set Up for the Right Question. Cancers (Basel). 2022;14: pubmed publisher
Heinrich A, Bhandary B, Potter S, Ratner N, DeFalco T. Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis. Cell Rep. 2021;37:109885 pubmed publisher
Larsen S, Cowley C, Sajjath S, Barrows D, Yang Y, Carroll T, et al. Establishment, maintenance, and recall of inflammatory memory. Cell Stem Cell. 2021;: pubmed publisher
Kawasaki K, Toshimitsu K, Matano M, Fujita M, Fujii M, Togasaki K, et al. An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping. Cell. 2020;183:1420-1435.e21 pubmed publisher
Barbar L, Jain T, Zimmer M, Kruglikov I, Sadick J, Wang M, et al. CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes. Neuron. 2020;107:436-453.e12 pubmed publisher
Romagnoli M, Bresson L, Di Cicco A, Pérez Lanzón M, Legoix P, Baulande S, et al. Laminin-binding integrins are essential for the maintenance of functional mammary secretory epithelium in lactation. Development. 2020;147: pubmed publisher
Ding L, Su Y, Fassl A, Hinohara K, Qiu X, Harper N, et al. Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. Nat Commun. 2019;10:4182 pubmed publisher
Liu L, Li Y, Guo N, Li S, Ma X, Zhang Y, et al. Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation. Cell Rep. 2019;27:455-466.e5 pubmed publisher
Mok K, Saxena N, Heitman N, Grisanti L, Srivastava D, Muraro M, et al. Dermal Condensate Niche Fate Specification Occurs Prior to Formation and Is Placode Progenitor Dependent. Dev Cell. 2019;48:32-48.e5 pubmed publisher
Hinohara K, Wu H, Vigneau S, McDonald T, Igarashi K, Yamamoto K, et al. KDM5 Histone Demethylase Activity Links Cellular Transcriptomic Heterogeneity to Therapeutic Resistance. Cancer Cell. 2018;34:939-953.e9 pubmed publisher
Nanki K, Toshimitsu K, Takano A, Fujii M, Shimokawa M, Ohta Y, et al. Divergent Routes toward Wnt and R-spondin Niche Independency during Human Gastric Carcinogenesis. Cell. 2018;174:856-869.e17 pubmed publisher
Evrard M, Kwok I, Chong S, Teng K, Becht E, Chen J, et al. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity. 2018;48:364-379.e8 pubmed publisher
Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, et al. Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression. Cell Stem Cell. 2018;22:454-467.e6 pubmed publisher
García Mariscal A, Li H, Pedersen E, Peyrollier K, Ryan K, Stanley A, et al. Loss of RhoA promotes skin tumor formation and invasion by upregulation of RhoB. Oncogene. 2017;: pubmed publisher
Shafer M, Nguyen A, Tremblay M, Viala S, Béland M, Bertos N, et al. Lineage Specification from Prostate Progenitor Cells Requires Gata3-Dependent Mitotic Spindle Orientation. Stem Cell Reports. 2017;8:1018-1031 pubmed publisher
Lowndes M, Rotherham M, Price J, El Haj A, Habib S. Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering. Stem Cell Reports. 2016;7:126-37 pubmed publisher
Sivasubramaniyan K, Harichandan A, Schilbach K, Mack A, Bedke J, Stenzl A, et al. Expression of stage-specific embryonic antigen-4 (SSEA-4) defines spontaneous loss of epithelial phenotype in human solid tumor cells. Glycobiology. 2015;25:902-17 pubmed publisher
Oeztuerk Winder F, Guinot A, Ochalek A, Ventura J. Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis. EMBO J. 2012;31:3431-41 pubmed publisher
Lai K, Yamashita S, Vitkus S, Shyr C, Yeh S, Chang C. Suppressed prostate epithelial development with impaired branching morphogenesis in mice lacking stromal fibromuscular androgen receptor. Mol Endocrinol. 2012;26:52-66 pubmed publisher
Grange C, Tapparo M, Collino F, Vitillo L, Damasco C, Deregibus M, et al. Microvesicles released from human renal cancer stem cells stimulate angiogenesis and formation of lung premetastatic niche. Cancer Res. 2011;71:5346-56 pubmed publisher
Jo M, Eastman B, Webb D, Stoletov K, Klemke R, Gonias S. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010;70:8948-58 pubmed publisher
Xu T, Xu Y, Liao C, Lau R, Goldkorn A. Reprogramming murine telomerase rapidly inhibits the growth of mouse cancer cells in vitro and in vivo. Mol Cancer Ther. 2010;9:438-49 pubmed publisher
Nardella C, Carracedo A, Alimonti A, Hobbs R, Clohessy J, Chen Z, et al. Differential requirement of mTOR in postmitotic tissues and tumorigenesis. Sci Signal. 2009;2:ra2 pubmed publisher
Horwitz K, Dye W, Harrell J, Kabos P, Sartorius C. Rare steroid receptor-negative basal-like tumorigenic cells in luminal subtype human breast cancer xenografts. Proc Natl Acad Sci U S A. 2008;105:5774-9 pubmed publisher
Stepp M, Liu Y, Pal Ghosh S, Jurjus R, Tadvalkar G, Sekaran A, et al. Reduced migration, altered matrix and enhanced TGFbeta1 signaling are signatures of mouse keratinocytes lacking Sdc1. J Cell Sci. 2007;120:2851-63 pubmed
product information
Antigen :
CD49f
Apps. Abbrev. :
FC, ICC, IHC-F, IP, Block
Cat # :
313602
Clone :
GoH3
Item :
Purified anti-human/mouse CD49f
Isotype :
Rat IgG2a, κ
Other Names :
VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Size :
100 μg
Price (USD) :
119 USD
Reactivity :
Human, Mouse, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
Mouse mammary tumor cells
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation^1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a6^1,4, and immunohistochemistry of acetone-fixed frozen sections^2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA