This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
LEAF™ Purified anti-human/mouse/rat CD278 (ICOS)
catalog :
313512
quantity :
500 μg
price :
280 USD
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
C398.4A
reactivity :
African green monkey, human, mouse, rat, rhesus macaque
application :
immunoprecipitation, flow cytometry, blocking or activating experiments, other
citations: 49
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s12a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Mantani P, Dunér P, Ljungcrantz I, Nilsson J, Bjorkbacka H, Fredrikson G. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol. 2019;20:47 pubmed publisher
  • other; human; loading ...; fig 7s1b
Saliba D, Céspedes Donoso P, Balint S, Compeer E, Korobchevskaya K, Valvo S, et al. Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Mizuno R, Sugiura D, Shimizu K, Maruhashi T, Watada M, Okazaki I, et al. PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation. Front Immunol. 2019;10:630 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
  • flow cytometry; rat; loading ...; fig 15a
Bath N, Ding X, Wilson N, Verhoven B, Boldt B, Sukhwal A, et al. Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model. PLoS ONE. 2019;14:e0211865 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 4d
Zhang C, Wang C, Jiang M, Gu C, Xiao J, Chen X, et al. Act1 is a negative regulator in T and B cells via direct inhibition of STAT3. Nat Commun. 2018;9:2745 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2c
Yi W, Gupta S, Ricker E, Manni M, Jessberger R, Chinenov Y, et al. The mTORC1-4E-BP-eIF4E axis controls de novo Bcl6 protein synthesis in T cells and systemic autoimmunity. Nat Commun. 2017;8:254 pubmed publisher
  • flow cytometry; human; loading ...; fig s2a
Martin Gayo E, Cronin J, Hickman T, Ouyang Z, Lindqvist M, Kolb K, et al. Circulating CXCR5+CXCR3+PD-1lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth. JCI Insight. 2017;2:e89574 pubmed publisher
  • flow cytometry; human
  • flow cytometry; mouse; loading ...; fig 2b
Kinosada H, Yasunaga J, Shimura K, Miyazato P, Onishi C, Iyoda T, et al. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS Pathog. 2017;13:e1006120 pubmed publisher
  • flow cytometry; mouse; loading ...
Ramirez Carrozzi V, Sambandam A, Zhou M, Yan D, Kang J, Wu X, et al. Combined blockade of the IL-13 and IL-33 pathways leads to a greater inhibition of type 2 inflammation over inhibition of either pathway alone. J Allergy Clin Immunol. 2017;139:705-708.e6 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Bal S, Bernink J, Nagasawa M, Groot J, Shikhagaie M, Golebski K, et al. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs. Nat Immunol. 2016;17:636-45 pubmed publisher
  • flow cytometry; human; loading ...; fig 3
Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
  • flow cytometry; human
Llibre A, López Macías C, Marafioti T, Mehta H, Partridge A, Kanzig C, et al. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation. J Immunol. 2016;196:2085-94 pubmed publisher
  • flow cytometry; African green monkey; fig 1
Vargas Inchaustegui D, Demers A, Shaw J, Kang G, Ball D, Tuero I, et al. Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques. J Immunol. 2016;196:1700-10 pubmed publisher
  • flow cytometry; mouse; fig s4
Kim J, Phan T, Nguyen V, Dinh Vu H, Zheng J, Yun M, et al. Salmonella typhimurium Suppresses Tumor Growth via the Pro-Inflammatory Cytokine Interleukin-1β. Theranostics. 2015;5:1328-42 pubmed publisher
  • blocking or activating experiments; mouse; 20 ug/ml; fig 7
Nelson M, Kundimi S, Bowers J, Rogers C, Huff L, Schwartz K, et al. The inducible costimulator augments Tc17 cell responses to self and tumor tissue. J Immunol. 2015;194:1737-47 pubmed publisher
  • flow cytometry; rat; fig 3
Xie L, Choudhury G, Winters A, Yang S, Jin K. Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10. Eur J Immunol. 2015;45:180-91 pubmed publisher
  • flow cytometry; human; 1:100
Gros A, Robbins P, Yao X, Li Y, Turcotte S, Tran E, et al. PD-1 identifies the patient-specific CD8? tumor-reactive repertoire infiltrating human tumors. J Clin Invest. 2014;124:2246-59 pubmed publisher
  • flow cytometry; mouse
Kawamoto S, Tran T, Maruya M, Suzuki K, Doi Y, Tsutsui Y, et al. The inhibitory receptor PD-1 regulates IgA selection and bacterial composition in the gut. Science. 2012;336:485-9 pubmed publisher
Chng M, Lim M, Rouers A, Becht E, Lee B, Macary P, et al. Large-Scale HLA Tetramer Tracking of T Cells during Dengue Infection Reveals Broad Acute Activation and Differentiation into Two Memory Cell Fates. Immunity. 2019;51:1119-1135.e5 pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Hu J, Wu J, Li Y, Wang Z, Tang J, Li Z, et al. Sclerostin domain-containing protein 1 is dispensable for the differentiation of follicular helper and follicular regulatory T cells during acute viral infection. Am J Transl Res. 2019;11:3722-3736 pubmed
Tripathi T, Yin W, Xue Y, Zurawski S, Fujita H, Hanabuchi S, et al. Central Roles of OX40L-OX40 Interaction in the Induction and Progression of Human T Cell-Driven Acute Graft-versus-Host Disease. Immunohorizons. 2019;3:110-120 pubmed publisher
Thauland T, Pellerin L, Ohgami R, Bacchetta R, Butte M. Case Study: Mechanism for Increased Follicular Helper T Cell Development in Activated PI3K Delta Syndrome. Front Immunol. 2019;10:753 pubmed publisher
Wagner J, Rapsomaniki M, Chevrier S, Anzeneder T, Langwieder C, Dykgers A, et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer. Cell. 2019;177:1330-1345.e18 pubmed publisher
Wong Y, Joshi K, Khetrapal P, Ismail M, Reading J, Sunderland M, et al. Urine-derived lymphocytes as a non-invasive measure of the bladder tumor immune microenvironment. J Exp Med. 2018;215:2748-2759 pubmed publisher
Preite S, Cannons J, Radtke A, Vujkovic Cvijin I, Gomez Rodriguez J, Volpi S, et al. Hyperactivated PI3Kδ promotes self and commensal reactivity at the expense of optimal humoral immunity. Nat Immunol. 2018;19:986-1000 pubmed publisher
Shi J, Hou S, Fang Q, Liu X, Liu X, Qi H. PD-1 Controls Follicular T Helper Cell Positioning and Function. Immunity. 2018;49:264-274.e4 pubmed publisher
Noto A, Procopio F, Banga R, Suffiotti M, Corpataux J, Cavassini M, et al. CD32+ and PD-1+ Lymph Node CD4 T Cells Support Persistent HIV-1 Transcription in Treated Aviremic Individuals. J Virol. 2018;92: pubmed publisher
Jogdand G, Sengupta S, Bhattacharya G, Singh S, Barik P, Devadas S. Inducible Costimulator Expressing T Cells Promote Parasitic Growth During Blood Stage Plasmodium berghei ANKA Infection. Front Immunol. 2018;9:1041 pubmed publisher
Valentine K, Davini D, Lawrence T, Mullins G, Manansala M, Al Kuhlani M, et al. CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease. J Immunol. 2018;201:31-40 pubmed publisher
Cortes J, Ambesi Impiombato A, Couronné L, Quinn S, Kim C, da Silva Almeida A, et al. RHOA G17V Induces T Follicular Helper Cell Specification and Promotes Lymphomagenesis. Cancer Cell. 2018;33:259-273.e7 pubmed publisher
Dutton E, Camelo A, Sleeman M, Herbst R, Carlesso G, Belz G, et al. Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity. Wellcome Open Res. 2017;2:117 pubmed publisher
Panzer S, Wilson N, Verhoven B, Xiang D, Rubinstein C, Redfield R, et al. Complete B Cell Deficiency Reduces Allograft Inflammation and Intragraft Macrophages a Rat Kidney Transplant Model. Transplantation. 2017;: pubmed publisher
Roussey J, Viglianti S, Teitz Tennenbaum S, Olszewski M, Osterholzer J. Anti-PD-1 Antibody Treatment Promotes Clearance of Persistent Cryptococcal Lung Infection in Mice. J Immunol. 2017;199:3535-3546 pubmed publisher
Nobs S, Natali S, Pohlmeier L, Okreglicka K, Schneider C, Kurrer M, et al. PPARγ in dendritic cells and T cells drives pathogenic type-2 effector responses in lung inflammation. J Exp Med. 2017;214:3015-3035 pubmed publisher
Mou W, Han W, Ma X, Wang X, Qin H, Zhao W, et al. γδTFH cells promote B cell maturation and antibody production in neuroblastoma. BMC Immunol. 2017;18:36 pubmed publisher
McDonnell A, Cook A, Robinson B, Lake R, Nowak A. Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters. BMC Cancer. 2017;17:417 pubmed publisher
Overacre Delgoffe A, Chikina M, Dadey R, Yano H, Brunazzi E, Shayan G, et al. Interferon-? Drives Treg Fragility to Promote Anti-tumor Immunity. Cell. 2017;169:1130-1141.e11 pubmed publisher
Klocke K, Holmdahl R, Wing K. CTLA-4 expressed by FOXP3+ regulatory T cells prevents inflammatory tissue attack and not T-cell priming in arthritis. Immunology. 2017;152:125-137 pubmed publisher
Chevrier S, Levine J, Zanotelli V, Silina K, Schulz D, Bacac M, et al. An Immune Atlas of Clear Cell Renal Cell Carcinoma. Cell. 2017;169:736-749.e18 pubmed publisher
Lu X, Horner J, Paul E, Shang X, Troncoso P, Deng P, et al. Effective combinatorial immunotherapy for castration-resistant prostate cancer. Nature. 2017;543:728-732 pubmed publisher
Ayala V, Deléage C, Trivett M, Jain S, Coren L, Breed M, et al. CXCR5-Dependent Entry of CD8 T Cells into Rhesus Macaque B-Cell Follicles Achieved through T-Cell Engineering. J Virol. 2017;91: pubmed publisher
Weinacht K, Charbonnier L, Alroqi F, Plant A, Qiao Q, Wu H, et al. Ruxolitinib reverses dysregulated T helper cell responses and controls autoimmunity caused by a novel signal transducer and activator of transcription 1 (STAT1) gain-of-function mutation. J Allergy Clin Immunol. 2017;139:1629-1640.e2 pubmed publisher
Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
Leeth C, Racine J, Chapman H, Arpa B, Carrillo J, Carrascal J, et al. B-lymphocytes expressing an Ig specificity recognizing the pancreatic ß-cell autoantigen peripherin are potent contributors to type 1 diabetes development in NOD mice. Diabetes. 2016;65:1977-1987 pubmed publisher
Xiao N, Eto D, Elly C, Peng G, Crotty S, Liu Y. The E3 ubiquitin ligase Itch is required for the differentiation of follicular helper T cells. Nat Immunol. 2014;15:657-66 pubmed publisher
Chen G, Hardy K, Bunting K, Daley S, Ma L, Shannon M. Regulation of the IL-21 gene by the NF-?B transcription factor c-Rel. J Immunol. 2010;185:2350-9 pubmed publisher
product information
Antigen :
CD278
Apps. Abbrev. :
Flow Cytometry, IP, Costim
Cat # :
313512
Clone :
C398.4A
Item :
LEAF™ Purified anti-human/mouse/rat CD278 (ICOS)
Isotype :
Armenian Hamster IgG
Other Names :
Inducible COStimulatory molecule, H4
Size :
500 μg
Price (USD) :
280 USD
Reactivity :
Human, African Green, Baboon, Cynomolgus, Mouse, Rat, Rhesus, Swine (Pig, Porcine)
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
LEAF
Immunogen :
Mouse T cell clone D10.G4.1
Application Notes :
The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 and in vitro costimulation of T cell activation 1,3,4 . The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA