TotalSeq™-A0405 anti-human CD284 (TLR4) | BioLegend 312817 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

TotalSeq™-A0405 anti-human CD284 (TLR4)

catalog :

312817

quantity :

10 μg

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HTA125

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2d	Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
	Hao Y, Hao S, Andersen Nissen E, Mauck W, Zheng S, Butler A, et al. Integrated analysis of multimodal single-cell data. Cell. 2021;184:3573-3587.e29 pubmed publisher
	Braun D, Dimayuga E, Morganti J, Van Eldik L. Microglial-associated responses to comorbid amyloid pathology and hyperhomocysteinemia in an aged knock-in mouse model of Alzheimer's disease. J Neuroinflammation. 2020;17:274 pubmed publisher

product information

Antigen :

CD284

Apps. Abbrev. :

Cat # :

312817

Clone :

HTA125

Item :

TotalSeq™-A0405 anti-human CD284 (TLR4)

Isotype :

Mouse IgG2a, κ

Other Names :

Toll-like Receptor 4, TLR4, TLR-4, CD284

Size :

10 μg

Price (USD) :

369 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

TOTALSEQ_A

Immunogen :

Ba/F3 cell line expressing human TLR4

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4, immunofluorescence microscopy^6, Western blotting^10, and in vitro blocking of LPS-induced cytokine production^2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).

more info or order :

BioLegend product webpage