APC anti-human CD284 (TLR4) | BioLegend 312815 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

APC anti-human CD284 (TLR4)

catalog :

312815

quantity :

25 tests

price :

149 USD

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

HTA125

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 5

Reference
Jost M, Jacobson A, Hussmann J, Cirolia G, Fischbach M, Weissman J. CRISPR-based functional genomics in human dendritic cells. elife. 2021;10: pubmed publisher
Gruenbacher G, Gander H, Dobler G, Rahm A, Klaver D, Thurnher M. The human G protein-coupled ATP receptor P2Y11 is a target for anti-inflammatory strategies. Br J Pharmacol. 2021;178:1541-1555 pubmed publisher
Torres Ruiz J, Carrillo Vazquez D, Padilla Ortiz D, Vázquez Rodríguez R, Nuñez Alvarez C, Juárez Vega G, et al. TLR expression in peripheral monocyte subsets of patients with idiopathic inflammatory myopathies: association with clinical and immunological features. J Transl Med. 2020;18:125 pubmed publisher
Wang Y, Chen C, Qi J, Wu F, Guan J, Chen Z, et al. Altered PGE2-EP2 is associated with an excessive immune response in HBV-related acute-on-chronic liver failure. J Transl Med. 2019;17:93 pubmed publisher
Wu X, Zhang G, Wang X, Zhang P, Tan Y. Endotoxin tolerance induction in human periodontal ligament fibroblasts stimulated with different bacterial lipopolysaccharides. Arch Oral Biol. 2015;60:463-70 pubmed publisher

product information

Antigen :

CD284

Apps. Abbrev. :

Cat # :

312815

Clone :

HTA125

Item :

APC anti-human CD284 (TLR4)

Isotype :

Mouse IgG2a, κ

Other Names :

Toll-like Receptor 4, TLR4, TLR-4, CD284

Size :

25 tests

Price (USD) :

149 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

APC

Immunogen :

Ba/F3 cell line expressing human TLR4

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4, immunofluorescence microscopy^6, Western blotting^10, and in vitro blocking of LPS-induced cytokine production^2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).

more info or order :

BioLegend product webpage