Ultra-LEAF™ Purified anti-human CD284 (TLR4) | BioLegend 312813 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-human CD284 (TLR4)

catalog :

312813

quantity :

100 μg

price :

237 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HTA125

reactivity :

human

application :

western blot, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2d	Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
	Lee Y, Reilly B, Tan C, Wang P, Aziz M. Extracellular CIRP Induces Macrophage Extracellular Trap Formation Via Gasdermin D Activation. Front Immunol. 2021;12:780210 pubmed publisher
	Thrash E, Kleinsteuber K, Hathaway E, Nazzaro M, Haas E, Hodi F, et al. High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples. STAR Protoc. 2020;1:100055 pubmed publisher
	Kumar R, Bunn P, Singh S, Ng S, Montes De Oca M, de Labastida Rivera F, et al. Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell Rep. 2020;30:2512-2525.e9 pubmed publisher

product information

Antigen :

CD284

Apps. Abbrev. :

FC, IHC-F, ICC, WB, Block

Cat # :

312813

Clone :

HTA125

Item :

Ultra-LEAF™ Purified anti-human CD284 (TLR4)

Isotype :

Mouse IgG2a, κ

Other Names :

Toll-like Receptor 4, TLR4, TLR-4, CD284

Size :

100 μg

Price (USD) :

237 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

ULEAF

Immunogen :

Ba/F3 cell line expressing human TLR4

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4, immunofluorescence microscopy^6, Western blotting^10, and in vitro blocking of LPS-induced cytokine production^2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).

more info or order :

BioLegend product webpage