Brilliant Violet 421™ anti-human CD284 (TLR4) | BioLegend 312811 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Brilliant Violet 421™ anti-human CD284 (TLR4)

catalog :

312811

quantity :

25 tests

price :

215 USD

clonality :

monoclonal

host :

mouse

conjugate :

BV421

clone name :

HTA125

reactivity :

human

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 4a	Hadadi E, Zhang B, Baidžajevas K, Yusof N, Puan K, Ong S, et al. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability. Sci Rep. 2016;6:39035 pubmed publisher
	Ramos C, Ballesteros Arias L, Silva J, Paiva R, Nogueira M, Carneiro J, et al. Cell Competition, the Kinetics of Thymopoiesis, and Thymus Cellularity Are Regulated by Double-Negative 2 to 3 Early Thymocytes. Cell Rep. 2020;32:107910 pubmed publisher
	He J, Yang Q, Xiao Q, Lei A, Li X, Zhou P, et al. IRF-7 Is a Critical Regulator of Type 2 Innate Lymphoid Cells in Allergic Airway Inflammation. Cell Rep. 2019;29:2718-2730.e6 pubmed publisher
	Viny A, Bowman R, Liu Y, Lavallée V, Eisman S, Xiao W, et al. Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation. Cell Stem Cell. 2019;25:682-696.e8 pubmed publisher
	Garaycoechea J, Crossan G, Langevin F, Mulderrig L, Louzada S, Yang F, et al. Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature. 2018;553:171-177 pubmed publisher
	Schaper F, de Leeuw K, Horst G, Bootsma H, Limburg P, Heeringa P, et al. High mobility group box 1 skews macrophage polarization and negatively influences phagocytosis of apoptotic cells. Rheumatology (Oxford). 2016;55:2260-2270 pubmed
	Glaser K, Fehrholz M, Curstedt T, Kunzmann S, Speer C. Effects of the New Generation Synthetic Reconstituted Surfactant CHF5633 on Pro- and Anti-Inflammatory Cytokine Expression in Native and LPS-Stimulated Adult CD14+ Monocytes. PLoS ONE. 2016;11:e0146898 pubmed publisher
	Bhattacharyya S, Gill R, Chen M, Zhang F, Linhardt R, Dudeja P, et al. Toll-like receptor 4 mediates induction of the Bcl10-NFkappaB-interleukin-8 inflammatory pathway by carrageenan in human intestinal epithelial cells. J Biol Chem. 2008;283:10550-8 pubmed publisher
	Chavakis E, Hain A, Vinci M, Carmona G, Bianchi M, Vajkoczy P, et al. High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells. Circ Res. 2007;100:204-12 pubmed

product information

Antigen :

CD284

Apps. Abbrev. :

Cat # :

312811

Clone :

HTA125

Item :

Brilliant Violet 421™ anti-human CD284 (TLR4)

Isotype :

Mouse IgG2a, κ

Other Names :

Toll-like Receptor 4, TLR4, TLR-4, CD284

Size :

25 tests

Price (USD) :

215 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

BV421

Immunogen :

Ba/F3 cell line expressing human TLR4

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4, immunofluorescence microscopy^6, Western blotting^10, and in vitro blocking of LPS-induced cytokine production^2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).

more info or order :

BioLegend product webpage