LEAF™ Purified anti-human CD284 (TLR4) | BioLegend 312807 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human CD284 (TLR4)

catalog :

312807

quantity :

100 μg

price :

200 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HTA125

reactivity :

human

application :

western blot, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

citations: 6

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2d	Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
flow cytometry; human; loading ...; fig 4a	Hadadi E, Zhang B, Baidžajevas K, Yusof N, Puan K, Ong S, et al. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability. Sci Rep. 2016;6:39035 pubmed publisher
blocking or activating experiments; human; loading ...; fig 3a	Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
	Moser J, Heeringa P, Jongman R, Zwiers P, Niemarkt A, Yan R, et al. Intracellular RIG-I Signaling Regulates TLR4-Independent Endothelial Inflammatory Responses to Endotoxin. J Immunol. 2016;196:4681-91 pubmed publisher
	Bhattacharyya S, Gill R, Chen M, Zhang F, Linhardt R, Dudeja P, et al. Toll-like receptor 4 mediates induction of the Bcl10-NFkappaB-interleukin-8 inflammatory pathway by carrageenan in human intestinal epithelial cells. J Biol Chem. 2008;283:10550-8 pubmed publisher
	Chavakis E, Hain A, Vinci M, Carmona G, Bianchi M, Vajkoczy P, et al. High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells. Circ Res. 2007;100:204-12 pubmed

product information

Antigen :

CD284

Apps. Abbrev. :

Flow Cytometry, IHC-F, ICC, Western Blotting, Block

Cat # :

312807

Clone :

HTA125

Item :

LEAF™ Purified anti-human CD284 (TLR4)

Isotype :

Mouse IgG2a, κ

Other Names :

Toll-like Receptor 4, TLR4, TLR-4, CD284

Size :

100 μg

Price (USD) :

200 USD

Reactivity :

Human, Cross-Reactivity: Guinea Pig

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Immunogen :

Ba/F3 cell line expressing human TLR4

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections 4 , immunofluorescence microscopy 6 , Western blotting 10 , and in vitro blocking of LPS-induced cytokine production 2,3,7,9 . This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 312807). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 312814) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).