Purified anti-human CD284 (TLR4) | BioLegend 312802 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-human CD284 (TLR4)

catalog :

312802

quantity :

100 μg

price :

197 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HTA125

reactivity :

human

application :

western blot, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2d	Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
western blot; human; loading ...; fig 1a	Hally K, La Flamme A, Larsen P, Harding S. Platelet Toll-like receptor (TLR) expression and TLR-mediated platelet activation in acute myocardial infarction. Thromb Res. 2017;158:8-15 pubmed publisher
flow cytometry; human; loading ...; fig 4a	Hadadi E, Zhang B, Baidžajevas K, Yusof N, Puan K, Ong S, et al. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability. Sci Rep. 2016;6:39035 pubmed publisher
blocking or activating experiments; human; loading ...; fig 2a	An L, Gorman J, Stephens G, Swerdlow B, Warrener P, Bonnell J, et al. Complement C5a induces PD-L1 expression and acts in synergy with LPS through Erk1/2 and JNK signaling pathways. Sci Rep. 2016;6:33346 pubmed publisher
blocking or activating experiments; human; fig 3	Pagano S, Carbone F, Burger F, Roth A, Bertolotto M, Pane B, et al. Anti-apolipoprotein A-1 auto-antibodies as active modulators of atherothrombosis. Thromb Haemost. 2016;116:554-64 pubmed publisher
blocking or activating experiments; human; loading ...; fig 3a	Cheng W, van Asten S, Burns L, Evans H, Walter G, Hashim A, et al. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses. Eur J Immunol. 2016;46:2211-21 pubmed publisher
	Feng J, Liu Y, Zhuang N, Chai Z, Liu L, Qian C, et al. EDA-E7 Activated DCs Induces Cytotoxic T Lymphocyte Immune Responses against HPV Expressing Cervical Cancer in Human Setting. Vaccines (Basel). 2023;11: pubmed publisher
	Waller K, Saihi H, Li W, Brindley J, de Jong A, Syn W, et al. Single-cell phenotypes of peripheral blood immune cells in early and late stages of non-alcoholic fatty liver disease. Clin Mol Hepatol. 2023;29:417-432 pubmed publisher
	Bai X, Xu P, Chen T, Zhang H, Wu S, Yang X, et al. The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis. BMC Immunol. 2022;23:42 pubmed publisher
	Heesters B, van Megesen K, Tomris I, de Vries R, Magri G, Spits H. Characterization of human FDCs reveals regulation of T cells and antigen presentation to B cells. J Exp Med. 2021;218: pubmed publisher
	Stephens M, Liao S, von der Weid P. Ultra-purification of Lipopolysaccharides reveals species-specific signalling bias of TLR4: importance in macrophage function. Sci Rep. 2021;11:1335 pubmed publisher
	Bennstein S, Weinhold S, Manser A, Scherenschlich N, Noll A, Raba K, et al. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells. elife. 2020;9: pubmed publisher
	Yoon J, Um H, Jang J, Bae Y, Park W, Kim H, et al. Eosinophil Activation by Toll-Like Receptor 4 Ligands Regulates Macrophage Polarization. Front Cell Dev Biol. 2019;7:329 pubmed publisher
	Poznanski S, Nham T, Chew M, Lee A, Hammill J, Fan I, et al. Expanded CD56superbrightCD16+ NK Cells from Ovarian Cancer Patients Are Cytotoxic against Autologous Tumor in a Patient-Derived Xenograft Murine Model. Cancer Immunol Res. 2018;6:1174-1185 pubmed publisher
	Alcántara Hernández M, Leylek R, Wagar L, Engleman E, Keler T, Marinkovich M, et al. High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization. Immunity. 2017;47:1037-1050.e6 pubmed publisher
	Manukyan G, Papajik T, Gajdos P, Mikulkova Z, Urbanová R, Gabcova G, et al. Neutrophils in chronic lymphocytic leukemia are permanently activated and have functional defects. Oncotarget. 2017;8:84889-84901 pubmed publisher
	Bhattacharyya S, Gill R, Chen M, Zhang F, Linhardt R, Dudeja P, et al. Toll-like receptor 4 mediates induction of the Bcl10-NFkappaB-interleukin-8 inflammatory pathway by carrageenan in human intestinal epithelial cells. J Biol Chem. 2008;283:10550-8 pubmed publisher
	Chavakis E, Hain A, Vinci M, Carmona G, Bianchi M, Vajkoczy P, et al. High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells. Circ Res. 2007;100:204-12 pubmed

product information

Antigen :

CD284

Apps. Abbrev. :

FC, IHC-F, ICC, WB, Block

Cat # :

312802

Clone :

HTA125

Item :

Purified anti-human CD284 (TLR4)

Isotype :

Mouse IgG2a, κ

Other Names :

Toll-like Receptor 4, TLR4, TLR-4, CD284

Size :

100 μg

Price (USD) :

197 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

PURE

Immunogen :

Ba/F3 cell line expressing human TLR4

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^4, immunofluorescence microscopy^6, Western blotting^10, and in vitro blocking of LPS-induced cytokine production^2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814).

more info or order :

BioLegend product webpage