Spark NIR™ 685 anti-human HLA-A,B,C | BioLegend 311454 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Spark NIR™ 685 anti-human HLA-A,B,C

catalog :

311454

quantity :

100 tests

price :

265 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

W6/32

reactivity :

human, rhesus macaque

application :

flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig s1	Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed publisher
blocking or activating experiments; human; 5 ug/ml; loading ...; fig 2e flow cytometry; human; loading ...; fig 2b	Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
	Mulder K, Patel A, Kong W, Piot C, Halitzki E, Dunsmore G, et al. Cross-tissue single-cell landscape of human monocytes and macrophages in health and disease. Immunity. 2021;54:1883-1900.e5 pubmed publisher
	Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher

product information

Antigen :

HLA-A

Apps. Abbrev. :

Cat # :

311454

Clone :

W6/32

Item :

Spark NIR™ 685 anti-human HLA-A,B,C

Isotype :

Mouse IgG2a, κ

Other Names :

Major Histocompatibility Class I, MHC class I

Size :

100 tests

Price (USD) :

265 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

SPKNIR_685

Application Notes :

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule^21.Additional reported applications (for the relevant formats) include: immunoprecipitaton^2, Western blotting (non-reducing)^3, immunohistochemical staining of acetone-fixed frozen tissue sections^4,5, blocking^6,7, inhibition of NK cell-mediated lysis^10, and activation^8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections^17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;

more info or order :

BioLegend product webpage