Ultra-LEAF™ Purified anti-human HLA-A,B,C | BioLegend 311428 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-human HLA-A,B,C

catalog :

311428

quantity :

1 mg

price :

149 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

W6/32

reactivity :

human, rhesus macaque

application :

western blot, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig s1	Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed publisher
blocking or activating experiments; human; 5 ug/ml; loading ...; fig 2e flow cytometry; human; loading ...; fig 2b	Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
flow cytometry; human; 1:75; loading ...; fig 2b	Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
flow cytometry; human; loading ...	Merola J, Reschke M, Pierce R, Qin L, Spindler S, Baltazar T, et al. Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. JCI Insight. 2019;4: pubmed publisher
flow cytometry; human; loading ...; fig s8	Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
flow cytometry; human; loading ...; fig s1	Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed publisher
flow cytometry; human; 1:50; fig 2a	Barkal A, Weiskopf K, Kao K, Gordon S, Rosental B, Yiu Y, et al. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018;19:76-84 pubmed publisher
flow cytometry; human; loading ...; fig s1b	Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
flow cytometry; human; loading ...; fig 3c	Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed publisher
	Lin W, Singh V, Springer R, Choonoo G, Gupta N, Patel A, et al. Human CD4 cytotoxic T lymphocytes mediate potent tumor control in humanized immune system mice. Commun Biol. 2023;6:447 pubmed publisher
	Baleeiro R, Bouwens C, Liu P, DI Gioia C, Dunmall L, Nagano A, et al. MHC class II molecules on pancreatic cancer cells indicate a potential for neo-antigen-based immunotherapy. Oncoimmunology. 2022;11:2080329 pubmed publisher
	Muik A, Garralda E, Altintas I, Gieseke F, Geva R, Ben Ami E, et al. Preclinical Characterization and Phase I Trial Results of a Bispecific Antibody Targeting PD-L1 and 4-1BB (GEN1046) in Patients with Advanced Refractory Solid Tumors. Cancer Discov. 2022;12:1248-1265 pubmed publisher
	Meng Q, Xie S, Gray G, Dezfulian M, Li W, Huang L, et al. Empirical identification and validation of tumor-targeting T cell receptors from circulation using autologous pancreatic tumor organoids. J Immunother Cancer. 2021;9: pubmed publisher
	Arakawa A, Reeves E, Vollmer S, Arakawa Y, He M, Galinski A, et al. ERAP1 Controls the Autoimmune Response against Melanocytes in Psoriasis by Generating the Melanocyte Autoantigen and Regulating Its Amount for HLA-C*06:02 Presentation. J Immunol. 2021;207:2235-2244 pubmed publisher
	Ito T, Kawai Y, Yasui Y, Iriguchi S, Minagawa A, Ishii T, et al. The therapeutic potential of multiclonal tumoricidal T cells derived from tumor infiltrating lymphocyte-1derived iPS cells. Commun Biol. 2021;4:694 pubmed publisher
	Eleftheriadis T, Pissas G, Crespo M, Filippidis G, Antoniadis N, Liakopoulos V, et al. The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation. Mol Med Rep. 2021;23: pubmed publisher
	Thrash E, Kleinsteuber K, Hathaway E, Nazzaro M, Haas E, Hodi F, et al. High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples. STAR Protoc. 2020;1:100055 pubmed publisher
	Kumar R, Bunn P, Singh S, Ng S, Montes De Oca M, de Labastida Rivera F, et al. Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell Rep. 2020;30:2512-2525.e9 pubmed publisher
	Dangaj D, Bruand M, Grimm A, Ronet C, Barras D, Duttagupta P, et al. Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors. Cancer Cell. 2019;35:885-900.e10 pubmed publisher
	Monel B, McKeon A, Lamothe Molina P, Jani P, Boucau J, Pacheco Y, et al. HIV Controllers Exhibit Effective CD8+ T Cell Recognition of HIV-1-Infected Non-activated CD4+ T Cells. Cell Rep. 2019;27:142-153.e4 pubmed publisher
	Jensen S, Potts G, Ready D, Patterson M. Specific MHC-I Peptides Are Induced Using PROTACs. Front Immunol. 2018;9:2697 pubmed publisher
	Laing B, Ross D, Meyer S, Campbell P, Halpin A, West L, et al. Glutaraldehyde treatment of allograft tissue decreases allosensitization after the Norwood procedure. J Thorac Cardiovasc Surg. 2010;139:1402-8 pubmed publisher
	Lünemann A, Lunemann J, Roberts S, Messmer B, Barreira da Silva R, Raine C, et al. Human NK cells kill resting but not activated microglia via NKG2D- and NKp46-mediated recognition. J Immunol. 2008;181:6170-7 pubmed
	McLoughlin R, Lee J, Kasper D, Tzianabos A. IFN-gamma regulated chemokine production determines the outcome of Staphylococcus aureus infection. J Immunol. 2008;181:1323-32 pubmed
	Takahara M, Miyai M, Tomiyama M, Mutou M, Nicol A, Nieda M. Copulsing tumor antigen-pulsed dendritic cells with zoledronate efficiently enhance the expansion of tumor antigen-specific CD8+ T cells via Vgamma9gammadelta T cell activation. J Leukoc Biol. 2008;83:742-54 pubmed

product information

Antigen :

HLA-A

Apps. Abbrev. :

FC, Activ, Block, IHC-F, IP, WB

Cat # :

311428

Clone :

W6/32

Item :

Ultra-LEAF™ Purified anti-human HLA-A,B,C

Isotype :

Mouse IgG2a, κ

Other Names :

Major Histocompatibility Class I, MHC class I

Size :

1 mg

Price (USD) :

149 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

ULEAF

Application Notes :

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule^21.Additional reported applications (for the relevant formats) include: immunoprecipitaton^2, Western blotting (non-reducing)^3, immunohistochemical staining of acetone-fixed frozen tissue sections^4,5, blocking^6,7, inhibition of NK cell-mediated lysis^10, and activation^8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections^17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;

more info or order :

BioLegend product webpage