This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
LEAF™ Purified anti-human HLA-A,B,C
catalog :
311423
quantity :
1 mg
price :
590 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
W6/32
reactivity :
human, chimpanzee, Rhesus monkey
application :
western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, blocking or activating experiments
citations: 20
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1
Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed publisher
  • flow cytometry; human; 1:50; fig 2a
Barkal A, Weiskopf K, Kao K, Gordon S, Rosental B, Yiu Y, et al. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018;19:76-84 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
  • blocking or activating experiments; human; loading ...
Zhang X, Lian X, Dai Z, Zheng H, Chen X, Zheng Y. ?3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2017;199:2030-2042 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1a
Johnson D, Wayt J, Wilson J, Donaldson J. Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking. J Cell Sci. 2017;130:2405-2415 pubmed publisher
  • flow cytometry; human; fig s2
Liu C, LeClair P, Monajemi M, Sly L, Reid G, Lim C. α-Integrin expression and function modulates presentation of cell surface calreticulin. Cell Death Dis. 2016;7:e2268 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed publisher
  • flow cytometry; human; fig s6b
Srivastava P, Paluch B, Matsuzaki J, James S, Collamat Lai G, Blagitko Dorfs N, et al. Induction of cancer testis antigen expression in circulating acute myeloid leukemia blasts following hypomethylating agent monotherapy. Oncotarget. 2016;7:12840-56 pubmed publisher
  • flow cytometry; human; 1:100; fig 2
Johnson D, Estrada M, Salgado R, Sanchez V, Doxie D, Opalenik S, et al. Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy. Nat Commun. 2016;7:10582 pubmed publisher
  • immunohistochemistry; human; 1:50
Yawata N, Selva K, Liu Y, Tan K, Lee A, Siak J, et al. Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection. Mucosal Immunol. 2016;9:159-70 pubmed publisher
  • flow cytometry; human; tbl 1
Boerman G, van Ostaijen Ten Dam M, Kraal K, Santos S, Ball L, Lankester A, et al. Role of NKG2D, DNAM-1 and natural cytotoxicity receptors in cytotoxicity toward rhabdomyosarcoma cell lines mediated by resting and IL-15-activated human natural killer cells. Cancer Immunol Immunother. 2015;64:573-83 pubmed publisher
  • flow cytometry; human; fig 1
  • immunocytochemistry; human; fig 3
Bradley S, Chen Z, Melendez B, Talukder A, Khalili J, Rodríguez Cruz T, et al. BRAFV600E Co-opts a Conserved MHC Class I Internalization Pathway to Diminish Antigen Presentation and CD8+ T-cell Recognition of Melanoma. Cancer Immunol Res. 2015;3:602-9 pubmed publisher
  • blocking or activating experiments; human; 15 ug/ml; fig 4
Tomimaru Y, Mishra S, Safran H, Charpentier K, Martin W, De Groot A, et al. Aspartate-β-hydroxylase induces epitope-specific T cell responses in hepatocellular carcinoma. Vaccine. 2015;33:1256-66 pubmed publisher
  • flow cytometry; human; fig 1
Boltjes A, van Montfoort N, Biesta P, Op den Brouw M, Kwekkeboom J, van der Laan L, et al. Kupffer cells interact with hepatitis B surface antigen in vivo and in vitro, leading to proinflammatory cytokine production and natural killer cell function. J Infect Dis. 2015;211:1268-78 pubmed publisher
  • flow cytometry; human
Matheson N, Peden A, Lehner P. Antibody-free magnetic cell sorting of genetically modified primary human CD4+ T cells by one-step streptavidin affinity purification. PLoS ONE. 2014;9:e111437 pubmed publisher
  • flow cytometry; human
Davey M, Morgan M, Liuzzi A, Tyler C, Khan M, Szakmany T, et al. Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells. J Immunol. 2014;193:3704-3716 pubmed publisher
  • flow cytometry; human
Vogel K, Thomann S, Vogel B, Schuster P, Schmidt B. Both plasmacytoid dendritic cells and monocytes stimulate natural killer cells early during human herpes simplex virus type 1 infections. Immunology. 2014;143:588-600 pubmed publisher
  • immunocytochemistry; human; 1:50
Noviello M, Tedesco F, Bondanza A, Tonlorenzi R, Rosaria Carbone M, Gerli M, et al. Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD. Mol Ther. 2014;22:1342-1352 pubmed publisher
  • western blot; human; fig 2
Kang W, Sung P, Park S, Yoon S, Chang D, Kim S, et al. Hepatitis C virus attenuates interferon-induced major histocompatibility complex class I expression and decreases CD8+ T cell effector functions. Gastroenterology. 2014;146:1351-60.e1-4 pubmed publisher
  • flow cytometry; human; loading ...; fig 2d
Svajger U, Obermajer N, Jeras M. IFN-?-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol. 2014;95:33-46 pubmed publisher
product information
Antigen :
HLA-A,B,C
Apps. Abbrev. :
Flow Cytometry, Activ, Block, IHC, IP, Western Blotting
Cat # :
311423
Clone :
W6/32
Item :
LEAF™ Purified anti-human HLA-A,B,C
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class I, MHC class I
Size :
1 mg
Price (USD) :
590 USD
Reactivity :
Human, African Green, Baboon, Cat (Feline), Cattle (Bovine, Cow), Chimpanzee, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
LEAF
Application Notes :
Clone W6/32 recognizes a monomorphic epitope on the 45 kD polypeptide products of HLA-A, B, C 18 . Additional reported applications (for the relevant formats) include: immunoprecipitaton 2 , Western blotting (non-reducing) 3 , immunohistochemical staining of acetone-fixed frozen tissue sections 4,5 , blocking 6,7 , inhibition of NK cell-mediated lysis 10 , and activation 8,9 . Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections 17 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 311412). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).
company information
BioLegend
9727 Pacific Heights Blvd.
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA