product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-human HLA-A,B,C
catalog :
311416
quantity :
25 tests
price :
103 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF647
clone name :
W6/32
reactivity :
human, rhesus macaque
application :
flow cytometry, blocking or activating experiments
more info or order :
citations: 29
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig s1
Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml; loading ...; fig 2e
  • flow cytometry; human; loading ...; fig 2b
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
  • flow cytometry; human; 1:75; loading ...; fig 2b
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
  • flow cytometry; human; loading ...
Merola J, Reschke M, Pierce R, Qin L, Spindler S, Baltazar T, et al. Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; human; loading ...; fig s8
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed publisher
  • flow cytometry; human; 1:50; fig 2a
Barkal A, Weiskopf K, Kao K, Gordon S, Rosental B, Yiu Y, et al. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018;19:76-84 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed publisher
Sparbier C, Gillespie A, Gomez J, Kumari N, Motazedian A, Chan K, et al. Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes. Nat Cell Biol. 2023;25:258-272 pubmed publisher
Stewart C, Gay C, Xi Y, Sivajothi S, Sivakamasundari V, Fujimoto J, et al. Single-cell analyses reveal increased intratumoral heterogeneity after the onset of therapy resistance in small-cell lung cancer. Nat Cancer. 2020;1:423-436 pubmed publisher
Gay C, Stewart C, Park E, Diao L, Groves S, Heeke S, et al. Patterns of transcription factor programs and immune pathway activation define four major subtypes of SCLC with distinct therapeutic vulnerabilities. Cancer Cell. 2021;39:346-360.e7 pubmed publisher
Bonf xe1 G, Cella F, Siciliano V. Engineering Protein-Based Parts for Genetic Devices in Mammalian Cells. Methods Mol Biol. 2021;2229:331-346 pubmed publisher
Souri Z, Jochemsen A, Versluis M, Wierenga A, Nemati F, van der Velden P, et al. HDAC Inhibition Increases HLA Class I Expression in Uveal Melanoma. Cancers (Basel). 2020;12: pubmed publisher
Cornel A, Szanto C, van Til N, van Velzen J, Boelens J, Nierkens S. A "No-Touch" Antibody-Staining Method of Adherent Cells for High-Throughput Flow Cytometry in 384-Well Microplate Format for Cell-Based Drug Library Screening. Cytometry A. 2020;97:845-851 pubmed publisher
Ma A, Motyka B, Gutfreund K, Shi Y, George R. A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Hum Vaccin Immunother. 2020;16:756-778 pubmed publisher
Bernson E, Christenson K, Pesce S, Pasanen M, Marcenaro E, Sivori S, et al. Downregulation of HLA Class I Renders Inflammatory Neutrophils More Susceptible to NK Cell-Induced Apoptosis. Front Immunol. 2019;10:2444 pubmed publisher
Gamage T, Schierding W, Tsai P, Ludgate J, Chamley L, Weeks R, et al. Human trophoblasts are primarily distinguished from somatic cells by differences in the pattern rather than the degree of global CpG methylation. Biol Open. 2018;7: pubmed publisher
Siciliano V, DiAndreth B, Monel B, Beal J, Huh J, Clayton K, et al. Engineering modular intracellular protein sensor-actuator devices. Nat Commun. 2018;9:1881 pubmed publisher
Cao J, Brouwer N, Jordanova E, Marinkovic M, van Duinen S, de Waard N, et al. HLA Class I Antigen Expression in Conjunctival Melanoma Is Not Associated With PD-L1/PD-1 Status. Invest Ophthalmol Vis Sci. 2018;59:1005-1015 pubmed publisher
Turco M, Gardner L, Hughes J, Cindrova Davies T, Gomez M, Farrell L, et al. Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. Nat Cell Biol. 2017;19:568-577 pubmed publisher
Thomas L, Vitale L, O Neill T, Dolnick R, Wallace P, Minderman H, et al. Development of a Novel Antibody-Drug Conjugate for the Potential Treatment of Ovarian, Lung, and Renal Cell Carcinoma Expressing TIM-1. Mol Cancer Ther. 2016;15:2946-2954 pubmed
James J, Hurley D, Gamage T, Zhang T, Vather R, Pantham P, et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction. 2015;150:449-62 pubmed publisher
Reichel J, Chadburn A, Rubinstein P, Giulino Roth L, Tam W, Liu Y, et al. Flow sorting and exome sequencing reveal the oncogenome of primary Hodgkin and Reed-Sternberg cells. Blood. 2015;125:1061-72 pubmed publisher
Porat Shliom N, Weigert R, Donaldson J. Endosomes derived from clathrin-independent endocytosis serve as precursors for endothelial lumen formation. PLoS ONE. 2013;8:e81987 pubmed publisher
Laing B, Ross D, Meyer S, Campbell P, Halpin A, West L, et al. Glutaraldehyde treatment of allograft tissue decreases allosensitization after the Norwood procedure. J Thorac Cardiovasc Surg. 2010;139:1402-8 pubmed publisher
Lünemann A, Lunemann J, Roberts S, Messmer B, Barreira da Silva R, Raine C, et al. Human NK cells kill resting but not activated microglia via NKG2D- and NKp46-mediated recognition. J Immunol. 2008;181:6170-7 pubmed
McLoughlin R, Lee J, Kasper D, Tzianabos A. IFN-gamma regulated chemokine production determines the outcome of Staphylococcus aureus infection. J Immunol. 2008;181:1323-32 pubmed
Takahara M, Miyai M, Tomiyama M, Mutou M, Nicol A, Nieda M. Copulsing tumor antigen-pulsed dendritic cells with zoledronate efficiently enhance the expansion of tumor antigen-specific CD8+ T cells via Vgamma9gammadelta T cell activation. J Leukoc Biol. 2008;83:742-54 pubmed
product information
Antigen :
HLA-A
Apps. Abbrev. :
FC
Cat # :
311416
Clone :
W6/32
Item :
Alexa Fluor® 647 anti-human HLA-A,B,C
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class I, MHC class I
Size :
25 tests
Price (USD) :
103 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A647
Application Notes :
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule^21.Additional reported applications (for the relevant formats) include: immunoprecipitaton^2, Western blotting (non-reducing)^3, immunohistochemical staining of acetone-fixed frozen tissue sections^4,5, blocking^6,7, inhibition of NK cell-mediated lysis^10, and activation^8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections^17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA