product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-human HLA-A,B,C
catalog :
311415
quantity :
25 tests
price :
103 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
W6/32
reactivity :
human, rhesus macaque
application :
flow cytometry, blocking or activating experiments
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig s1
Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml; loading ...; fig 2e
  • flow cytometry; human; loading ...; fig 2b
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
  • flow cytometry; human; 1:75; loading ...; fig 2b
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
  • flow cytometry; human; loading ...
Merola J, Reschke M, Pierce R, Qin L, Spindler S, Baltazar T, et al. Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; human; loading ...; fig s8
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed publisher
  • flow cytometry; human; 1:50; fig 2a
Barkal A, Weiskopf K, Kao K, Gordon S, Rosental B, Yiu Y, et al. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018;19:76-84 pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed publisher
  • flow cytometry; human; 1:100; fig 2
Johnson D, Estrada M, Salgado R, Sanchez V, Doxie D, Opalenik S, et al. Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy. Nat Commun. 2016;7:10582 pubmed publisher
Sparbier C, Gillespie A, Gomez J, Kumari N, Motazedian A, Chan K, et al. Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes. Nat Cell Biol. 2023;25:258-272 pubmed publisher
Simmons S, Lithwick Yanai G, Adiconis X, Oberstrass F, Iremadze N, Geiger Schuller K, et al. Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing. Nat Biotechnol. 2023;41:204-211 pubmed publisher
Karvas R, Khan S, Verma S, Yin Y, Kulkarni D, Dong C, et al. Stem-cell-derived trophoblast organoids model human placental development and susceptibility to emerging pathogens. Cell Stem Cell. 2022;29:810-825.e8 pubmed publisher
Sheridan M, Zhao X, Fernando R, Gardner L, Perez García V, Li Q, et al. Characterization of primary models of human trophoblast. Development. 2021;148: pubmed publisher
Larsen B, Kannan M, Langer L, Leibowitz B, BenTaieb A, Cancino A, et al. A pan-cancer organoid platform for precision medicine. Cell Rep. 2021;36:109429 pubmed publisher
Sheridan M, Fernando R, Gardner L, Hollinshead M, Burton G, Moffett A, et al. Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta. Nat Protoc. 2020;15:3441-3463 pubmed publisher
Stopfer L, Mesfin J, Joughin B, Lauffenburger D, White F. Multiplexed relative and absolute quantitative immunopeptidomics reveals MHC I repertoire alterations induced by CDK4/6 inhibition. Nat Commun. 2020;11:2760 pubmed publisher
Queckborner S, Syk Lundberg E, Gemzell Danielsson K, Davies L. Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile. Stem Cell Res Ther. 2020;11:15 pubmed publisher
Burr M, Sparbier C, Chan K, Chan Y, Kersbergen A, Lam E, et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell. 2019;36:385-401.e8 pubmed publisher
Yeong J, Lim J, Lee B, Li H, Ong C, Thike A, et al. Prognostic value of CD8 + PD-1+ immune infiltrates and PDCD1 gene expression in triple negative breast cancer. J Immunother Cancer. 2019;7:34 pubmed publisher
Eisemann T, Costa B, Harter P, Wick W, Mittelbronn M, Angel P, et al. Podoplanin expression is a prognostic biomarker but may be dispensable for the malignancy of glioblastoma. Neuro Oncol. 2019;21:326-336 pubmed publisher
Luo N, Nixon M, Gonzalez Ericsson P, Sanchez V, Opalenik S, Li H, et al. DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer. Nat Commun. 2018;9:248 pubmed publisher
Turco M, Gardner L, Hughes J, Cindrova Davies T, Gomez M, Farrell L, et al. Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium. Nat Cell Biol. 2017;19:568-577 pubmed publisher
Nobutani K, Shimono Y, Mizutani K, Ueda Y, Suzuki T, Kitayama M, et al. Downregulation of CXCR4 in Metastasized Breast Cancer Cells and Implication in Their Dormancy. PLoS ONE. 2015;10:e0130032 pubmed publisher
Lowes L, Hedley B, Keeney M, Allan A. Adaptation of semiautomated circulating tumor cell (CTC) assays for clinical and preclinical research applications. J Vis Exp. 2014;:e51248 pubmed publisher
Lee J, Lee J, Farquhar K, Yun J, Frankenberger C, Bevilacqua E, et al. Network of mutually repressive metastasis regulators can promote cell heterogeneity and metastatic transitions. Proc Natl Acad Sci U S A. 2014;111:E364-73 pubmed publisher
Goodridge J, Lee N, Burian A, Pyo C, Tykodi S, Warren E, et al. HLA-F and MHC-I open conformers cooperate in a MHC-I antigen cross-presentation pathway. J Immunol. 2013;191:1567-77 pubmed publisher
Hokanson D, Bretscher A. EPI64 interacts with Slp1/JFC1 to coordinate Rab8a and Arf6 membrane trafficking. Mol Biol Cell. 2012;23:701-15 pubmed publisher
Laing B, Ross D, Meyer S, Campbell P, Halpin A, West L, et al. Glutaraldehyde treatment of allograft tissue decreases allosensitization after the Norwood procedure. J Thorac Cardiovasc Surg. 2010;139:1402-8 pubmed publisher
Lünemann A, Lunemann J, Roberts S, Messmer B, Barreira da Silva R, Raine C, et al. Human NK cells kill resting but not activated microglia via NKG2D- and NKp46-mediated recognition. J Immunol. 2008;181:6170-7 pubmed
McLoughlin R, Lee J, Kasper D, Tzianabos A. IFN-gamma regulated chemokine production determines the outcome of Staphylococcus aureus infection. J Immunol. 2008;181:1323-32 pubmed
Takahara M, Miyai M, Tomiyama M, Mutou M, Nicol A, Nieda M. Copulsing tumor antigen-pulsed dendritic cells with zoledronate efficiently enhance the expansion of tumor antigen-specific CD8+ T cells via Vgamma9gammadelta T cell activation. J Leukoc Biol. 2008;83:742-54 pubmed
product information
Antigen :
HLA-A
Apps. Abbrev. :
FC
Cat # :
311415
Clone :
W6/32
Item :
Alexa Fluor® 488 anti-human HLA-A,B,C
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class I, MHC class I
Size :
25 tests
Price (USD) :
103 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A488
Application Notes :
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule^21.Additional reported applications (for the relevant formats) include: immunoprecipitaton^2, Western blotting (non-reducing)^3, immunohistochemical staining of acetone-fixed frozen tissue sections^4,5, blocking^6,7, inhibition of NK cell-mediated lysis^10, and activation^8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections^17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA