LEAF™ Purified anti-human HLA-A,B,C | BioLegend 311412 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human HLA-A,B,C

catalog :

311412

quantity :

500 μg

price :

320 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

W6/32

reactivity :

human, chimpanzee, Rhesus monkey

application :

western blot, immunohistochemistry, neutralization, immunoprecipitation, flow cytometry

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig s1	Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed publisher
flow cytometry; human; 1:50; fig 2a	Barkal A, Weiskopf K, Kao K, Gordon S, Rosental B, Yiu Y, et al. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018;19:76-84 pubmed publisher
flow cytometry; human; loading ...; fig s1b	Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
flow cytometry; human; loading ...; fig 3c	Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed publisher
	Everds N, Li N, Bailey K, Fort M, Stevenson R, Jawando R, et al. Unexpected thrombocytopenia and anemia in cynomolgus monkeys induced by a therapeutic human monoclonal antibody. Toxicol Pathol. 2013;41:951-69 pubmed publisher
	Wei C, Chung W, Huang H, Chen Y, Hung S. Direct interaction between HLA-B and carbamazepine activates T cells in patients with Stevens-Johnson syndrome. J Allergy Clin Immunol. 2012;129:1562-9.e5 pubmed publisher
	Laing B, Ross D, Meyer S, Campbell P, Halpin A, West L, et al. Glutaraldehyde treatment of allograft tissue decreases allosensitization after the Norwood procedure. J Thorac Cardiovasc Surg. 2010;139:1402-8 pubmed publisher
	Lünemann A, Lunemann J, Roberts S, Messmer B, Barreira da Silva R, Raine C, et al. Human NK cells kill resting but not activated microglia via NKG2D- and NKp46-mediated recognition. J Immunol. 2008;181:6170-7 pubmed
	McLoughlin R, Lee J, Kasper D, Tzianabos A. IFN-gamma regulated chemokine production determines the outcome of Staphylococcus aureus infection. J Immunol. 2008;181:1323-32 pubmed
	Takahara M, Miyai M, Tomiyama M, Mutou M, Nicol A, Nieda M. Copulsing tumor antigen-pulsed dendritic cells with zoledronate efficiently enhance the expansion of tumor antigen-specific CD8+ T cells via Vgamma9gammadelta T cell activation. J Leukoc Biol. 2008;83:742-54 pubmed

product information

Antigen :

HLA-A,B,C

Apps. Abbrev. :

Flow Cytometry, Activ, Block, IHC, IP, Western Blotting

Cat # :

311412

Clone :

W6/32

Item :

LEAF™ Purified anti-human HLA-A,B,C

Isotype :

Mouse IgG2a, κ

Other Names :

Major Histocompatibility Class I, MHC class I

Size :

500 μg

Price (USD) :

320 USD

Reactivity :

Human, African Green, Baboon, Cat (Feline), Cattle (Bovine, Cow), Chimpanzee, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

Clone W6/32 recognizes a monomorphic epitope on the 45 kD polypeptide products of HLA-A, B, C 18 . Additional reported applications (for the relevant formats) include: immunoprecipitaton 2 , Western blotting (non-reducing) 3 , immunohistochemical staining of acetone-fixed frozen tissue sections 4,5 , blocking 6,7 , inhibition of NK cell-mediated lysis 10 , and activation 8,9 . Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections 17 . The LEAF purified antibody (Endotoxin 0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 311412). For highly sensitive assays, we recommend Ultra-LEAF purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF purified antibodies (Endotoxin 0.01 EU/ug).