product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human HLA-A,B,C
catalog :
311410
quantity :
100 tests
price :
291 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
W6/32
reactivity :
human, mouse, rhesus macaque
application :
immunocytochemistry, flow cytometry, other
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:200; loading ...; fig 3d
Wu S, Xiao Y, Wei J, Xu X, Jin X, Hu X, et al. MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer. J Immunother Cancer. 2021;9: pubmed publisher
  • other; mouse; 1:100; fig 3c
Nathan A, Rossin E, Kaseke C, Park R, Khatri A, Koundakjian D, et al. Structure-guided T cell vaccine design for SARS-CoV-2 variants and sarbecoviruses. Cell. 2021;: pubmed publisher
  • flow cytometry; human; loading ...; fig 2e, 2f, 2g
Zhang Y, Chen Y, Li Y, Huang F, Luo B, Yuan Y, et al. The ORF8 protein of SARS-CoV-2 mediates immune evasion through down-regulating MHC-Ι. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; human; loading ...; fig 3
Heusinger E, Deppe K, Sette P, Krapp C, Kmiec D, Kluge S, et al. Preadaptation of Simian Immunodeficiency Virus SIVsmm Facilitated Env-Mediated Counteraction of Human Tetherin by Human Immunodeficiency Virus Type 2. J Virol. 2018;92: pubmed publisher
  • flow cytometry; human; fig 1
  • immunocytochemistry; human; fig 3
Bradley S, Chen Z, Melendez B, Talukder A, Khalili J, Rodríguez Cruz T, et al. BRAFV600E Co-opts a Conserved MHC Class I Internalization Pathway to Diminish Antigen Presentation and CD8+ T-cell Recognition of Melanoma. Cancer Immunol Res. 2015;3:602-9 pubmed publisher
Murayama T, Nakayama J, Jiang X, Miyata K, Morris A, Cai K, et al. Targeting DHX9 triggers tumor-intrinsic interferon response and replication stress in Small Cell Lung Cancer. Cancer Discov. 2024;: pubmed publisher
Tunal x131 G, R xfa bies Bed xf3 s M, Nagarajan D, Fridh P, Papakyriacou I, Mao Y. IL-1 receptor-associated kinase-3 acts as an immune checkpoint in myeloid cells to limit cancer immunotherapy. J Clin Invest. 2023;133: pubmed publisher
Sparbier C, Gillespie A, Gomez J, Kumari N, Motazedian A, Chan K, et al. Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes. Nat Cell Biol. 2023;25:258-272 pubmed publisher
Gerace D, Zhou Q, Kenty J, Veres A, Sintov E, Wang X, et al. Engineering human stem cell-derived islets to evade immune rejection and promote localized immune tolerance. Cell Rep Med. 2023;4:100879 pubmed publisher
Yoon H, Kim G, Oh S, Kim H, Kim Y, Lee Y, et al. WNK3 inhibition elicits antitumor immunity by suppressing PD-L1 expression on tumor cells and activating T-cell function. Exp Mol Med. 2022;54:1913-1926 pubmed publisher
Lian W, Wang Z, Ma Y, Tong Y, Zhang X, Jin H, et al. FABP6 Expression Correlates with Immune Infiltration and Immunogenicity in Colorectal Cancer Cells. J Immunol Res. 2022;2022:3129765 pubmed publisher
Lee P, Klaeger S, Le P, Korthauer K, Cheng J, Ananthapadmanabhan V, et al. Reversal of viral and epigenetic HLA class I repression in Merkel cell carcinoma. J Clin Invest. 2022;132: pubmed publisher
Amo L, D xed ez Garc xed a J, Tamayo Orbegozo E, Maruri N, Larrucea S. Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site. Front Immunol. 2022;13:835527 pubmed publisher
Ren J, Li N, Pei S, Lian Y, Li L, Peng Y, et al. Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated anti-tumor immunity. J Clin Invest. 2022;: pubmed publisher
Kemble S, Dalby A, Lowe G, Nicolson P, Watson S, Senis Y, et al. Analysis of preplatelets and their barbell platelet derivatives by imaging flow cytometry. Blood Adv. 2022;6:2932-2946 pubmed publisher
Hoke A, Padget M, Fabian K, Nandal A, Gallia G, Bilusic M, et al. Combinatorial Natural Killer Cell-based Immunotherapy Approaches Selectively Target Chordoma Cancer Stem Cells. Cancer Res Commun. 2021;1:127-139 pubmed publisher
Choi J, Goulding S, Conn B, McGann C, Dietze J, Kohler J, et al. Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations. Cell Rep Methods. 2021;1:100084 pubmed publisher
Bear A, Blanchard T, Cesare J, Ford M, Richman L, Xu C, et al. Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targeting. Nat Commun. 2021;12:4365 pubmed publisher
Kaseke C, Park R, Singh N, Koundakjian D, Bashirova A, Garcia Beltran W, et al. HLA class-I-peptide stability mediates CD8+ T cell immunodominance hierarchies and facilitates HLA-associated immune control of HIV. Cell Rep. 2021;36:109378 pubmed publisher
Massafra V, Tundo S, Dietzig A, Ducret A, Jost C, Klein C, et al. Proteolysis-Targeting Chimeras Enhance T Cell Bispecific Antibody-Driven T Cell Activation and Effector Function through Increased MHC Class I Antigen Presentation in Cancer Cells. J Immunol. 2021;207:493-504 pubmed publisher
Yamaguchi S, Hamana H, Shitaoka K, Sukegawa K, Nagata T, Hayee A, et al. TCR function analysis using a novel system reveals the multiple unconventional tumor-reactive T cells in human breast cancer-infiltrating lymphocytes. Eur J Immunol. 2021;51:2306-2316 pubmed publisher
Charles A, Bourne C, Korontsvit T, Aretz Z, Mun S, Dao T, et al. Low-dose CDK4/6 inhibitors induce presentation of pathway specific MHC ligands as potential targets for cancer immunotherapy. Oncoimmunology. 2021;10:1916243 pubmed publisher
Bondarenko M, Le Grand M, Shaked Y, Raviv Z, Chapuisat G, Carr xe8 re C, et al. Metronomic Chemotherapy Modulates Clonal Interactions to Prevent Drug Resistance in Non-Small Cell Lung Cancer. Cancers (Basel). 2021;13: pubmed publisher
Io S, Kabata M, Iemura Y, Semi K, Morone N, Minagawa A, et al. Capturing human trophoblast development with naive pluripotent stem cells in vitro. Cell Stem Cell. 2021;28:1023-1039.e13 pubmed publisher
Slaine P, Kleer M, Duguay B, Pringle E, Kadijk E, Ying S, et al. Thiopurines activate an antiviral unfolded protein response that blocks influenza A virus glycoprotein accumulation. J Virol. 2021;: pubmed publisher
Mandrup O, Ong S, Lykkemark S, Dinesen A, Rudnik Jansen I, Dagnæs Hansen N, et al. Programmable half-life and anti-tumour effects of bispecific T-cell engager-albumin fusions with tuned FcRn affinity. Commun Biol. 2021;4:310 pubmed publisher
Okita R, Shimizu K, Nojima Y, Saisho S, Nakata M. Tofacitinib overcomes an IFNγ-induced decrease in NK cell-mediated cytotoxicity via the regulation of immune-related molecules in LC-2/ad. Thorac Cancer. 2021;12:775-782 pubmed publisher
Ng W, Gong C, Yan X, Si G, Fang C, Wang L, et al. Targeting CD155 by rediocide-A overcomes tumour immuno-resistance to natural killer cells. Pharm Biol. 2021;59:47-53 pubmed publisher
Bagati A, Kumar S, Jiang P, Pyrdol J, Zou A, Godicelj A, et al. Integrin αvβ6-TGFβ-SOX4 Pathway Drives Immune Evasion in Triple-Negative Breast Cancer. Cancer Cell. 2021;39:54-67.e9 pubmed publisher
Bachireddy P, Ennis C, Nguyen V, Gohil S, Clement K, Shukla S, et al. Distinct evolutionary paths in chronic lymphocytic leukemia during resistance to the graft-versus-leukemia effect. Sci Transl Med. 2020;12: pubmed publisher
Sim M, Lu J, Spencer M, Hopkins F, Tran E, Rosenberg S, et al. High-affinity oligoclonal TCRs define effective adoptive T cell therapy targeting mutant KRAS-G12D. Proc Natl Acad Sci U S A. 2020;117:12826-12835 pubmed publisher
Dheilly E, Battistello E, Katanayeva N, Sungalee S, Michaux J, Duns G, et al. Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma. Cancer Cell. 2020;37:674-689.e12 pubmed publisher
Lee J, Shklovskaya E, Lim S, Carlino M, Menzies A, Stewart A, et al. Transcriptional downregulation of MHC class I and melanoma de- differentiation in resistance to PD-1 inhibition. Nat Commun. 2020;11:1897 pubmed publisher
Ferrari de Andrade L, Kumar S, Luoma A, Ito Y, Alves da Silva P, Pan D, et al. Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells. Cancer Immunol Res. 2020;8:769-780 pubmed publisher
Preece R, Georgiadis C, Gkazi S, Etuk A, Christi A, Qasim W. 'Mini' U6 Pol III promoter exhibits nucleosome redundancy and supports multiplexed coupling of CRISPR/Cas9 effects. Gene Ther. 2020;27:451-458 pubmed publisher
Han P, Hanlon D, Arshad N, Lee J, Tatsuno K, Robinson E, et al. Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Sci Adv. 2020;6:eaaz1580 pubmed publisher
Joas S, Sauermann U, Roshani B, Klippert A, Daskalaki M, Mätz Rensing K, et al. Nef-Mediated CD3-TCR Downmodulation Dampens Acute Inflammation and Promotes SIV Immune Evasion. Cell Rep. 2020;30:2261-2274.e7 pubmed publisher
Rapiteanu R, Karagyozova T, Zimmermann N, Singh K, Wayne G, Martufi M, et al. Highly efficient genome editing in primary human bronchial epithelial cells differentiated at air-liquid interface. Eur Respir J. 2020;55: pubmed publisher
Zhuang X, Veltri D, Long E. Genome-Wide CRISPR Screen Reveals Cancer Cell Resistance to NK Cells Induced by NK-Derived IFN-γ. Front Immunol. 2019;10:2879 pubmed publisher
Burr M, Sparbier C, Chan K, Chan Y, Kersbergen A, Lam E, et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell. 2019;36:385-401.e8 pubmed publisher
Giles A, Hao S, Padget M, Song H, Zhang W, Lynes J, et al. Efficient ADCC killing of meningioma by avelumab and a high-affinity natural killer cell line, haNK. JCI Insight. 2019;4: pubmed publisher
Kula T, Dezfulian M, Wang C, Abdelfattah N, Hartman Z, Wucherpfennig K, et al. T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes. Cell. 2019;178:1016-1028.e13 pubmed publisher
Li Y, Zhao C, Liu J, Lu Z, Lu M, Gu J, et al. CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity. Oncol Rep. 2019;41:2679-2688 pubmed publisher
Usmani S, Murooka T, Deruaz M, Koh W, Sharaf R, Di Pilato M, et al. HIV-1 Balances the Fitness Costs and Benefits of Disrupting the Host Cell Actin Cytoskeleton Early after Mucosal Transmission. Cell Host Microbe. 2019;25:73-86.e5 pubmed publisher
Yao C, Ni Z, Gong C, Zhu X, Wang L, Xu Z, et al. Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy. Autophagy. 2018;14:1831-1844 pubmed publisher
Mitchell K, Barreyro L, Todorova T, Taylor S, Antony Debré I, Narayanagari S, et al. IL1RAP potentiates multiple oncogenic signaling pathways in AML. J Exp Med. 2018;215:1709-1727 pubmed publisher
Joas S, Parrish E, Gnanadurai C, Lump E, Stürzel C, Parrish N, et al. Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity. Nat Commun. 2018;9:1371 pubmed publisher
Alvino V, Fernández Jiménez R, Rodriguez Arabaolaza I, Slater S, Mangialardi G, Avolio E, et al. Transplantation of Allogeneic Pericytes Improves Myocardial Vascularization and Reduces Interstitial Fibrosis in a Swine Model of Reperfused Acute Myocardial Infarction. J Am Heart Assoc. 2018;7: pubmed publisher
Shan X, Fung J, Kosaka A, Danet Desnoyers G. Replication Study: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. elife. 2017;6: pubmed publisher
Ho J, Pelzel C, Begitt A, Mee M, Elsheikha H, Scott D, et al. STAT2 Is a Pervasive Cytokine Regulator due to Its Inhibition of STAT1 in Multiple Signaling Pathways. PLoS Biol. 2016;14:e2000117 pubmed publisher
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Rosskopf S, Jutz S, Neunkirchner A, Candia M, Jahn Schmid B, Bohle B, et al. Creation of an engineered APC system to explore and optimize the presentation of immunodominant peptides of major allergens. Sci Rep. 2016;6:31580 pubmed publisher
Hashimoto M, Bhuyan F, Hiyoshi M, Noyori O, Nasser H, Miyazaki M, et al. Potential Role of the Formation of Tunneling Nanotubes in HIV-1 Spread in Macrophages. J Immunol. 2016;196:1832-41 pubmed publisher
Hashimoto M, Nasser H, Bhuyan F, Kuse N, Satou Y, Harada S, et al. Fibrocytes Differ from Macrophages but Can Be Infected with HIV-1. J Immunol. 2015;195:4341-50 pubmed publisher
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McLoughlin R, Lee J, Kasper D, Tzianabos A. IFN-gamma regulated chemokine production determines the outcome of Staphylococcus aureus infection. J Immunol. 2008;181:1323-32 pubmed
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product information
Antigen :
HLA-A
Apps. Abbrev. :
FC
Cat # :
311410
Clone :
W6/32
Item :
APC anti-human HLA-A,B,C
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class I, MHC class I
Size :
100 tests
Price (USD) :
291 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Application Notes :
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule^21.Additional reported applications (for the relevant formats) include: immunoprecipitaton^2, Western blotting (non-reducing)^3, immunohistochemical staining of acetone-fixed frozen tissue sections^4,5, blocking^6,7, inhibition of NK cell-mediated lysis^10, and activation^8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections^17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA