product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human HLA-A,B,C
catalog :
311402
quantity :
100 μg
price :
89 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
W6/32
reactivity :
human, mouse, rhesus macaque
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments, other, mass cytometry
more info or order :
citations: 90
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig s1
Poletto E, Colella P, Pimentel Vera L, Khan S, Tomatsu S, Baldo G, et al. Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. Mol Ther Methods Clin Dev. 2022;25:392-409 pubmed publisher
  • mass cytometry; human; loading ...; fig 5b
van der Heide V, Jangra S, Cohen P, Rathnasinghe R, Aslam S, Aydillo T, et al. Limited extent and consequences of pancreatic SARS-CoV-2 infection. Cell Rep. 2022;38:110508 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 6
Han E, Qian H, Jiang B, Figetakis M, Kosyakova N, Tellides G, et al. A therapeutic vascular conduit to support in vivo cell-secreted therapy. NPJ Regen Med. 2021;6:40 pubmed publisher
  • blocking or activating experiments; human; 5 ug/ml; loading ...; fig 2e
  • flow cytometry; human; loading ...; fig 2b
Zhu Y, Xie J, Shi J. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis. BMC Biol. 2021;19:140 pubmed publisher
  • ELISA; human
Hsiue E, Wright K, Douglass J, Hwang M, Mog B, Pearlman A, et al. Targeting a neoantigen derived from a common TP53 mutation. Science. 2021;371: pubmed publisher
  • flow cytometry; human; 1:75; loading ...; fig 2b
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
  • flow cytometry; human; loading ...
Merola J, Reschke M, Pierce R, Qin L, Spindler S, Baltazar T, et al. Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; human; loading ...; fig s8
Pavel Dinu M, Wiebking V, Dejene B, Srifa W, Mantri S, Nicolas C, et al. Gene correction for SCID-X1 in long-term hematopoietic stem cells. Nat Commun. 2019;10:1634 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed publisher
  • flow cytometry; human; 1:50; fig 2a
Barkal A, Weiskopf K, Kao K, Gordon S, Rosental B, Yiu Y, et al. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018;19:76-84 pubmed publisher
  • flow cytometry; human; loading ...; fig 5b
Mwimanzi F, Toyoda M, Mahiti M, Mann J, Martin J, Bangsberg D, et al. Resistance of Major Histocompatibility Complex Class B (MHC-B) to Nef-Mediated Downregulation Relative to that of MHC-A Is Conserved among Primate Lentiviruses and Influences Antiviral T Cell Responses in HIV-1-Infected Individuals. J Virol. 2018;92: pubmed publisher
  • flow cytometry; human; loading ...; fig s1b
Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
  • blocking or activating experiments; human; loading ...
Zhang X, Lian X, Dai Z, Zheng H, Chen X, Zheng Y. ?3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2017;199:2030-2042 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1a
Johnson D, Wayt J, Wilson J, Donaldson J. Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking. J Cell Sci. 2017;130:2405-2415 pubmed publisher
  • flow cytometry; human; fig e6a
Dever D, Bak R, Reinisch A, Camarena J, Washington G, Nicolas C, et al. CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells. Nature. 2016;539:384-389 pubmed publisher
  • flow cytometry; human; loading ...; fig 3e, 3f
Albanese M, Tagawa T, Bouvet M, Maliqi L, Lutter D, Hoser J, et al. Epstein-Barr virus microRNAs reduce immune surveillance by virus-specific CD8+ T cells. Proc Natl Acad Sci U S A. 2016;113:E6467-E6475 pubmed
  • flow cytometry; human; loading ...
Tagawa T, Albanese M, Bouvet M, Moosmann A, Mautner J, Heissmeyer V, et al. Epstein-Barr viral miRNAs inhibit antiviral CD4+ T cell responses targeting IL-12 and peptide processing. J Exp Med. 2016;213:2065-80 pubmed publisher
  • flow cytometry; human; fig 1a
Sadallah S, Schmied L, Eken C, Charoudeh H, Amicarella F, Schifferli J. Platelet-Derived Ectosomes Reduce NK Cell Function. J Immunol. 2016;197:1663-71 pubmed publisher
  • flow cytometry; human; fig s2
Liu C, LeClair P, Monajemi M, Sly L, Reid G, Lim C. α-Integrin expression and function modulates presentation of cell surface calreticulin. Cell Death Dis. 2016;7:e2268 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Reches A, Nachmani D, Berhani O, Duev Cohen A, Shreibman D, Ophir Y, et al. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins. J Immunol. 2016;196:4967-76 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016;196:4064-74 pubmed publisher
  • flow cytometry; human; fig s6b
Srivastava P, Paluch B, Matsuzaki J, James S, Collamat Lai G, Blagitko Dorfs N, et al. Induction of cancer testis antigen expression in circulating acute myeloid leukemia blasts following hypomethylating agent monotherapy. Oncotarget. 2016;7:12840-56 pubmed publisher
  • flow cytometry; human; 1:100; fig 2
Johnson D, Estrada M, Salgado R, Sanchez V, Doxie D, Opalenik S, et al. Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy. Nat Commun. 2016;7:10582 pubmed publisher
  • immunohistochemistry; human; 1:50
Yawata N, Selva K, Liu Y, Tan K, Lee A, Siak J, et al. Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection. Mucosal Immunol. 2016;9:159-70 pubmed publisher
  • other; human; fig 2
Jørgensen M, Bæk R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048 pubmed publisher
  • flow cytometry; human; tbl 1
Boerman G, van Ostaijen Ten Dam M, Kraal K, Santos S, Ball L, Lankester A, et al. Role of NKG2D, DNAM-1 and natural cytotoxicity receptors in cytotoxicity toward rhabdomyosarcoma cell lines mediated by resting and IL-15-activated human natural killer cells. Cancer Immunol Immunother. 2015;64:573-83 pubmed publisher
  • flow cytometry; human; fig 1
  • immunocytochemistry; human; fig 3
Bradley S, Chen Z, Melendez B, Talukder A, Khalili J, Rodríguez Cruz T, et al. BRAFV600E Co-opts a Conserved MHC Class I Internalization Pathway to Diminish Antigen Presentation and CD8+ T-cell Recognition of Melanoma. Cancer Immunol Res. 2015;3:602-9 pubmed publisher
  • ELISA; human; fig s1
Nambiar J, Clarke A, Shim D, Mabon D, Tian C, Windloch K, et al. Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model. MAbs. 2015;7:638-50 pubmed publisher
  • blocking or activating experiments; human; 15 ug/ml; fig 4
Tomimaru Y, Mishra S, Safran H, Charpentier K, Martin W, De Groot A, et al. Aspartate-β-hydroxylase induces epitope-specific T cell responses in hepatocellular carcinoma. Vaccine. 2015;33:1256-66 pubmed publisher
  • flow cytometry; human; fig 1
Boltjes A, van Montfoort N, Biesta P, Op den Brouw M, Kwekkeboom J, van der Laan L, et al. Kupffer cells interact with hepatitis B surface antigen in vivo and in vitro, leading to proinflammatory cytokine production and natural killer cell function. J Infect Dis. 2015;211:1268-78 pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 3
Jung Y, Wang J, Lee E, McGee S, Berry J, Yumoto K, et al. Annexin 2-CXCL12 interactions regulate metastatic cell targeting and growth in the bone marrow. Mol Cancer Res. 2015;13:197-207 pubmed publisher
  • western blot; human; fig 2
Kang W, Sung P, Park S, Yoon S, Chang D, Kim S, et al. Hepatitis C virus attenuates interferon-induced major histocompatibility complex class I expression and decreases CD8+ T cell effector functions. Gastroenterology. 2014;146:1351-60.e1-4 pubmed publisher
  • flow cytometry; human; loading ...; fig 2d
Svajger U, Obermajer N, Jeras M. IFN-?-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol. 2014;95:33-46 pubmed publisher
Menevse A, Ammer L, Vollmann Zwerenz A, Kupczyk M, Lorenz J, Weidner L, et al. TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastoma. Acta Neuropathol Commun. 2023;11:75 pubmed publisher
Khatri A, Todd J, Kelly F, Nagler A, Ji Z, Jain V, et al. JAK-STAT activation contributes to cytotoxic T cell-mediated basal cell death in human chronic lung allograft dysfunction. JCI Insight. 2023;8: pubmed publisher
Moon J, Younis S, Ramadoss N, Iyer R, Sheth K, Sharpe O, et al. Cytotoxic CD8+ T cells target citrullinated antigens in rheumatoid arthritis. Nat Commun. 2023;14:319 pubmed publisher
Gallen C, Dukes C, Aldrich A, Macaisa L, Mo Q, Cubitt C, et al. Long-Term CD4+ T-Cell and Immunoglobulin G Immune Responses in Oncology Workers following COVID-19 Vaccination: An Interim Analysis of a Prospective Cohort Study. Vaccines (Basel). 2022;10: pubmed publisher
Jimenez Duran G, Kozole J, Peltier Heap R, Dickinson E, Kwiatkowski C, Zappacosta F, et al. Complement membrane attack complex is an immunometabolic regulator of NLRP3 activation and IL-18 secretion in human macrophages. Front Immunol. 2022;13:918551 pubmed publisher
Chou J, Tsai J, Hung J, Chen I, Chen S, Tsai M. The ORF8 Protein of SARS-CoV-2 Modulates the Spike Protein and Its Implications in Viral Transmission. Front Microbiol. 2022;13:883597 pubmed publisher
Sim M, Stotz Z, Lu J, Brennan P, Long E, Sun P. T cells discriminate between groups C1 and C2 HLA-C. elife. 2022;11: pubmed publisher
Moquin Beaudry G, Benabdallah B, Maggiorani D, Le O, Li Y, Colas C, et al. Autologous humanized mouse models of iPSC-derived tumors enable characterization and modulation of cancer-immune cell interactions. Cell Rep Methods. 2022;2:100153 pubmed publisher
Zhu Z, Teng K, Zhou J, Xu Y, Zhang L, Zhao H, et al. B7H6 Serves as a Negative Prognostic Marker and an Immune Modulator in Human Pancreatic Cancer. Front Oncol. 2022;12:814312 pubmed publisher
Ganan Gomez I, Yang H, Ma F, Montalban Bravo G, Thongon N, Marchica V, et al. Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy. Nat Med. 2022;28:557-567 pubmed publisher
Higashi S, Makiyama T, Sakane H, Nogami S, Shirataki H. Regulation of Hook1-mediated endosomal sorting of clathrin-independent cargo by γ-taxilin. J Cell Sci. 2022;135: pubmed publisher
Eiva M, Omran D, Chacon J, Powell D. Systematic analysis of CD39, CD103, CD137, and PD-1 as biomarkers for naturally occurring tumor antigen-specific TILs. Eur J Immunol. 2022;52:96-108 pubmed publisher
Neuperger P, Balog J, Tiszlavicz L, Fur xe1 k J, G xe9 mes N, Kotog xe1 ny E, et al. Analysis of the Single-Cell Heterogeneity of Adenocarcinoma Cell Lines and the Investigation of Intratumor Heterogeneity Reveals the Expression of Transmembrane Protein 45A (TMEM45A) in Lung Adenocarcinoma Cancer Patients. Cancers (Basel). 2021;14: pubmed publisher
Nosaka K, Suzuki S, Yoshikawa T, Shimomura M, Kitami K, Yoshida K, et al. Heat Shock Protein 105 as an Immunotherapeutic Target for Patients With Cervical Cancer. Anticancer Res. 2021;41:4741-4751 pubmed publisher
Song S, Manook M, Kwun J, Jackson A, Knechtle S, Kelsoe G. Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells. Front Immunol. 2021;12:705140 pubmed publisher
Pettmann J, Huhn A, Abu Shah E, Kutuzov M, Wilson D, Dustin M, et al. The discriminatory power of the T cell receptor. elife. 2021;10: pubmed publisher
Willimsky G, Beier C, Immisch L, Papafotiou G, Scheuplein V, Goede A, et al. In vitro proteasome processing of neo-splicetopes does not predict their presentation in vivo. elife. 2021;10: pubmed publisher
Ruiz Cuevas M, Hardy M, Hollý J, Bonneil E, Durette C, Courcelles M, et al. Most non-canonical proteins uniquely populate the proteome or immunopeptidome. Cell Rep. 2021;34:108815 pubmed publisher
Mirlashari M, Vetlesen A, Nissen Meyer L, Naper C, Tj xf8 nnfjord G, Njerve I, et al. HLA class I depletion by citric acid, and irradiation of apheresis platelets for transfusion of refractory patients. Transfusion. 2021;61:1222-1234 pubmed publisher
Tanaka Y, Wada H, Goto R, Osada T, Yamamura K, Fukaya S, et al. TAS0314, a novel multi-epitope long peptide vaccine, showed synergistic antitumor immunity with PD-1/PD-L1 blockade in HLA-A*2402 mice. Sci Rep. 2020;10:17284 pubmed publisher
Nguyen R, Patel A, Griffiths L, Dapper J, Stewart E, Houston J, et al. Next-generation humanized patient-derived xenograft mouse model for pre-clinical antibody studies in neuroblastoma. Cancer Immunol Immunother. 2021;70:721-732 pubmed publisher
Parker R, Partridge T, Wormald C, Kawahara R, Stalls V, Aggelakopoulou M, et al. Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells. bioRxiv. 2020;: pubmed publisher
Pierini S, Tanyi J, Simpkins F, George E, Uribe Herranz M, Drapkin R, et al. Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target. JCI Insight. 2020;5: pubmed publisher
Dinh H, Eggert T, Meyer M, Zhu Y, Olingy C, Llewellyn R, et al. Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow. Immunity. 2020;53:319-334.e6 pubmed publisher
Rennier K, Shin W, Krug E, Virdi G, Pachynski R. Chemerin Reactivates PTEN and Suppresses PD-L1 in Tumor Cells via Modulation of a Novel CMKLR1-mediated Signaling Cascade. Clin Cancer Res. 2020;26:5019-5035 pubmed publisher
Sharma M, Zhang S, Niu L, Lewinsohn D, Zhang X, Huang S. Mucosal-Associated Invariant T Cells Develop an Innate-Like Transcriptomic Program in Anti-mycobacterial Responses. Front Immunol. 2020;11:1136 pubmed publisher
Cohen M, Zhang S, Sengupta S, Ma H, Bell G, Horton B, et al. Formation of Human Neuroblastoma in Mouse-Human Neural Crest Chimeras. Cell Stem Cell. 2020;26:579-592.e6 pubmed publisher
Eccles J, Turner R, Kirk N, Muehling L, Borish L, Steinke J, et al. T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection. Cell Rep. 2020;30:351-366.e7 pubmed publisher
Chen Y, Gomes T, Hardman C, Vieira Braga F, Gutowska Owsiak D, Salimi M, et al. Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation. J Exp Med. 2020;217: pubmed publisher
Martin J, Chang C, Boschetti G, Ungaro R, Giri M, Grout J, et al. Single-Cell Analysis of Crohn's Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy. Cell. 2019;178:1493-1508.e20 pubmed publisher
Makarkov A, Golizeh M, Ruiz Lancheros E, Gopal A, Costas Cancelas I, Chierzi S, et al. Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages. NPJ Vaccines. 2019;4:17 pubmed publisher
Carlsten M, Namazi A, Reger R, Levy E, Berg M, St Hilaire C, et al. Bortezomib sensitizes multiple myeloma to NK cells via ER-stress-induced suppression of HLA-E and upregulation of DR5. Oncoimmunology. 2019;8:e1534664 pubmed publisher
Abdul Salam V, Russomanno G, Chien Nien C, Mahomed A, Yates L, Wilkins M, et al. CLIC4/Arf6 Pathway. Circ Res. 2019;124:52-65 pubmed publisher
Fu J, Zuber J, Martinez M, Shonts B, Obradovic A, Wang H, et al. Human Intestinal Allografts Contain Functional Hematopoietic Stem and Progenitor Cells that Are Maintained by a Circulating Pool. Cell Stem Cell. 2019;24:227-239.e8 pubmed publisher
Wroblewska A, Dhainaut M, Ben Zvi B, Rose S, Park E, Amir E, et al. Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens. Cell. 2018;175:1141-1155.e16 pubmed publisher
Garrido C, Clohosey M, Whitworth C, Hudgens M, Margolis D, Soriano Sarabia N. γδ T cells: an immunotherapeutic approach for HIV cure strategies. JCI Insight. 2018;3: pubmed publisher
Fitzgerald W, Gomez Lopez N, Erez O, Romero R, Margolis L. Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors. Am J Reprod Immunol. 2018;80:e12860 pubmed publisher
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product information
Antigen :
HLA-A
Apps. Abbrev. :
FC, Activ, Block, IHC-F, IP, WB
Cat # :
311402
Clone :
W6/32
Item :
Purified anti-human HLA-A,B,C
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class I, MHC class I
Size :
100 μg
Price (USD) :
89 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Application Notes :
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule^21.Additional reported applications (for the relevant formats) include: immunoprecipitaton^2, Western blotting (non-reducing)^3, immunohistochemical staining of acetone-fixed frozen tissue sections^4,5, blocking^6,7, inhibition of NK cell-mediated lysis^10, and activation^8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections^17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA