product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD69 (Maxpar® Ready)
catalog :
310939
quantity :
100 μg
price :
129 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FN50
reactivity :
human, mouse, chimpanzee, rhesus macaque
application :
flow cytometry, other, mass cytometry
more info or order :
citations: 42
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; loading ...; fig 4c
Wang H, Chen L, Qi L, Jiang N, Zhang Z, Guo H, et al. A Single-Cell Atlas of Tumor-Infiltrating Immune Cells in Pancreatic Ductal Adenocarcinoma. Mol Cell Proteomics. 2022;21:100258 pubmed publisher
  • mass cytometry; human; loading ...; fig s4a
Jiang Z, Qin L, Tang Y, Liao R, Shi J, He B, et al. Human induced-T-to-natural killer cells have potent anti-tumour activities. Biomark Res. 2022;10:13 pubmed publisher
  • flow cytometry; human; fig 5
Hawerkamp H, Domdey A, Radau L, Sewerin P, Oláh P, Homey B, et al. Tofacitinib downregulates antiviral immune defence in keratinocytes and reduces T cell activation. Arthritis Res Ther. 2021;23:144 pubmed publisher
  • mass cytometry; human; loading ...; fig 4e
  • flow cytometry; human; loading ...; fig 3b
Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • other; mouse
Tseng H, Xiong W, Badeti S, Yang Y, Ma M, Liu T, et al. Efficacy of anti-CD147 chimeric antigen receptors targeting hepatocellular carcinoma. Nat Commun. 2020;11:4810 pubmed publisher
  • flow cytometry; human; loading ...; fig s3b
Fischer M, Ruhnau J, Schulze J, Obst D, Floel A, Vogelgesang A. Spermine and spermidine modulate T-cell function in older adults with and without cognitive decline ex vivo. Aging (Albany NY). 2020;12:13716-13739 pubmed publisher
  • flow cytometry; human; loading ...; fig 3c
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Humeniuk P, Geiselhart S, Battin C, Webb T, Steinberger P, Paster W, et al. Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor. Sci Rep. 2019;9:7426 pubmed publisher
  • flow cytometry; human; loading ...; fig s2
Swaims Kohlmeier A, Haddad L, Li Z, Brookmeyer K, Baker J, Widom C, et al. Chronic immune barrier dysregulation among women with a history of violence victimization. JCI Insight. 2019;4: pubmed publisher
  • flow cytometry; human; loading ...; fig 7a
Lichnog C, Klabunde S, Becker E, Fuh F, Tripal P, Atreya R, et al. Cellular Mechanisms of Etrolizumab Treatment in Inflammatory Bowel Disease. Front Pharmacol. 2019;10:39 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5e
Hayashi T, Momota M, Kuroda E, Kusakabe T, Kobari S, Makisaka K, et al. DAMP-Inducing Adjuvant and PAMP Adjuvants Parallelly Enhance Protective Type-2 and Type-1 Immune Responses to Influenza Split Vaccination. Front Immunol. 2018;9:2619 pubmed publisher
  • flow cytometry; human; loading ...; fig 4c
Ye W, Chew M, Hou J, Lai F, Leopold S, Loo H, et al. Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway. PLoS Pathog. 2018;14:e1007298 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a, 5b
Cooper G, Ostridge K, Khakoo S, Wilkinson T, Staples K. Human CD49a+ Lung Natural Killer Cell Cytotoxicity in Response to Influenza A Virus. Front Immunol. 2018;9:1671 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Pizzolla A, Nguyen T, Sant S, Jaffar J, Loudovaris T, Mannering S, et al. Influenza-specific lung-resident memory T cells are proliferative and polyfunctional and maintain diverse TCR profiles. J Clin Invest. 2018;128:721-733 pubmed publisher
  • flow cytometry; human; loading ...; fig 2f
Herndler Brandstetter D, Shan L, Yao Y, Stecher C, Plajer V, Lietzenmayer M, et al. Humanized mouse model supports development, function, and tissue residency of human natural killer cells. Proc Natl Acad Sci U S A. 2017;114:E9626-E9634 pubmed publisher
  • flow cytometry; human; loading ...; fig 4b
Matos T, O Malley J, Lowry E, Hamm D, Kirsch I, Robins H, et al. Clinically resolved psoriatic lesions contain psoriasis-specific IL-17-producing ?? T cell clones. J Clin Invest. 2017;127:4031-4041 pubmed publisher
  • mass cytometry; human; loading ...; fig 2a
Chew V, Lai L, Pan L, Lim C, Li J, Ong R, et al. Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses. Proc Natl Acad Sci U S A. 2017;114:E5900-E5909 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Dias J, Leeansyah E, Sandberg J. Multiple layers of heterogeneity and subset diversity in human MAIT cell responses to distinct microorganisms and to innate cytokines. Proc Natl Acad Sci U S A. 2017;114:E5434-E5443 pubmed publisher
  • flow cytometry; human; loading ...; fig s2
Su S, Zou Z, Chen F, Ding N, Du J, Shao J, et al. CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer. Oncoimmunology. 2017;6:e1249558 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...
Roberts E, Carnathan D, Li H, Shaw G, Silvestri G, Betts M. Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathog. 2016;12:e1006135 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Kadivar M, Petersson J, Svensson L, Marsal J. CD8??+ ?? T Cells: A Novel T Cell Subset with a Potential Role in Inflammatory Bowel Disease. J Immunol. 2016;197:4584-4592 pubmed
  • flow cytometry; human; fig s5
Pachnio A, Ciáurriz M, Begum J, Lal N, Zuo J, Beggs A, et al. Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS Pathog. 2016;12:e1005832 pubmed publisher
  • flow cytometry; rhesus macaque; tbl 1
Williams D, Engle E, Shirk E, Queen S, Gama L, Mankowski J, et al. Splenic Damage during SIV Infection: Role of T-Cell Depletion and Macrophage Polarization and Infection. Am J Pathol. 2016;186:2068-2087 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig s3a
Rueda C, Presicce P, Jackson C, Miller L, Kallapur S, Jobe A, et al. Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque. J Immunol. 2016;196:3706-15 pubmed publisher
  • flow cytometry; human; loading ...; fig s9
Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
  • flow cytometry; human; fig 2
Offersen R, Nissen S, Rasmussen T, Østergaard L, Denton P, Søgaard O, et al. A Novel Toll-Like Receptor 9 Agonist, MGN1703, Enhances HIV-1 Transcription and NK Cell-Mediated Inhibition of HIV-1-Infected Autologous CD4+ T Cells. J Virol. 2016;90:4441-4453 pubmed publisher
  • flow cytometry; human; fig 4
Setoguchi R. IL-15 boosts the function and migration of human terminally differentiated CD8+ T cells by inducing a unique gene signature. Int Immunol. 2016;28:293-305 pubmed publisher
  • flow cytometry; human; fig 5
Su S, Hu B, Shao J, Shen B, Du J, Du Y, et al. CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients. Sci Rep. 2016;6:20070 pubmed publisher
  • flow cytometry; human; loading ...; fig 3d
Tham M, Schlör G, Yerly D, Mueller C, Surbek D, Villiger P, et al. Reduced pro-inflammatory profile of γδT cells in pregnant patients with rheumatoid arthritis. Arthritis Res Ther. 2016;18:26 pubmed publisher
  • flow cytometry; human; fig 1
Whisenant T, Peralta E, Aarreberg L, Gao N, Head S, Ordoukhanian P, et al. The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells. PLoS ONE. 2015;10:e0144409 pubmed publisher
  • flow cytometry; human; fig s3
Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, et al. Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus. J Immunol. 2015;194:3890-900 pubmed publisher
  • flow cytometry; human; fig 7
Phadnis Moghe A, Crawford R, Kaminski N. Suppression of human B cell activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin involves altered regulation of B cell lymphoma-6. Toxicol Sci. 2015;144:39-50 pubmed publisher
  • flow cytometry; human
Setoguchi R, Matsui Y, Mouri K. mTOR signaling promotes a robust and continuous production of IFN-γ by human memory CD8+ T cells and their proliferation. Eur J Immunol. 2015;45:893-902 pubmed publisher
  • flow cytometry; human
Jitschin R, Braun M, Büttner M, Dettmer Wilde K, Bricks J, Berger J, et al. CLL-cells induce IDOhi CD14+HLA-DRlo myeloid-derived suppressor cells that inhibit T-cell responses and promote TRegs. Blood. 2014;124:750-60 pubmed publisher
  • flow cytometry; chimpanzee
Hodara V, Parodi L, Chavez D, Smith L, Lanford R, Giavedoni L. Characterization of ??T cells in naïve and HIV-infected chimpanzees and their responses to T-cell activators in vitro. J Med Primatol. 2014;43:258-71 pubmed publisher
Michelozzi I, Sufi J, Adejumo T, Amrolia P, Tape C, Giustacchini A. High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry. STAR Protoc. 2022;3:101174 pubmed publisher
Loo Yau H, Bell E, Ettayebi I, de Almeida F, Boukhaled G, Shen S, et al. DNA hypomethylating agents increase activation and cytolytic activity of CD8+ T cells. Mol Cell. 2021;81:1469-1483.e8 pubmed publisher
Stras S, Werner L, Toothaker J, Olaloye O, Oldham A, McCourt C, et al. Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development. Dev Cell. 2019;51:357-373.e5 pubmed publisher
Gide T, Quek C, Menzies A, Tasker A, Shang P, Holst J, et al. Distinct Immune Cell Populations Define Response to Anti-PD-1 Monotherapy and Anti-PD-1/Anti-CTLA-4 Combined Therapy. Cancer Cell. 2019;35:238-255.e6 pubmed publisher
Lu H, Crawford R, North C, Kaplan B, Kaminski N. Establishment of an immunoglobulin m antibody-forming cell response model for characterizing immunotoxicity in primary human B cells. Toxicol Sci. 2009;112:363-73 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
product information
Antigen :
CD69
Apps. Abbrev. :
FC, CyTOF®
Cat # :
310939
Clone :
FN50
Item :
Purified anti-human CD69 (Maxpar® Ready)
Isotype :
Mouse IgG1, κ
Other Names :
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Size :
100 μg
Price (USD) :
129 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PUREMAX
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections^2, immunofluorescence microscopy^3, and spatial biology (IBEX)^8,9.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA