Alexa Fluor® 647 anti-human CD137 (4-1BB) | BioLegend 309823 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Alexa Fluor® 647 anti-human CD137 (4-1BB)

catalog :

309823

quantity :

25 tests

price :

147 USD

clonality :

monoclonal

host :

mouse

conjugate :

AF647

clone name :

4B4-1

reactivity :

human

application :

flow cytometry, blocking or activating experiments

more info or order :

BioLegend product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3a	Pei Y, Xiang Z, Wen K, Tu C, Wang X, Zhang Y, et al. CD137 Costimulation Enhances the Antitumor Activity of Vγ9Vδ2-T Cells in IL-10-Mediated Immunosuppressive Tumor Microenvironment. Front Immunol. 2022;13:872122 pubmed publisher
flow cytometry; human	Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
flow cytometry; human; 3:50; loading ...; fig 3j	Park J, Botting R, Domínguez Conde C, Popescu D, Lavaert M, Kunz D, et al. A cell atlas of human thymic development defines T cell repertoire formation. Science. 2020;367: pubmed publisher
flow cytometry; human; fig 3g	Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
flow cytometry; human; loading ...; fig s2b	Scheper W, Kelderman S, Fanchi L, Linnemann C, Bendle G, de Rooij M, et al. Low and variable tumor reactivity of the intratumoral TCR repertoire in human cancers. Nat Med. 2019;25:89-94 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Gabanti E, Bruno F, Scaramuzzi L, Mangione F, Zelini P, Gerna G, et al. Predictive value of human cytomegalovirus (HCMV) T-cell response in the control of HCMV infection by seropositive solid-organ transplant recipients according to different assays and stimuli. New Microbiol. 2016;39:247-258 pubmed
flow cytometry; human; fig 6	Jutz S, Leitner J, Schmetterer K, Doel Perez I, Majdic O, Grabmeier Pfistershammer K, et al. Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-ÎºB, NFAT and AP-1. J Immunol Methods. 2016;430:10-20 pubmed publisher
blocking or activating experiments; human; 10 ug/ml; fig 4	Flinsenberg T, Spel L, Jansen M, Koning D, de Haar C, Plantinga M, et al. Cognate CD4 T-cell licensing of dendritic cells heralds anti-cytomegalovirus CD8 T-cell immunity after human allogeneic umbilical cord blood transplantation. J Virol. 2015;89:1058-69 pubmed publisher
	Charmetant X, Espi M, Benotmane I, Barateau V, Heibel F, Buron F, et al. Infection or a third dose of mRNA vaccine elicits neutralizing antibody responses against SARS-CoV-2 in kidney transplant recipients. Sci Transl Med. 2022;14:eabl6141 pubmed publisher
	Nguyen J, Pettmann J, Kruger P, Dushek O. Quantitative contributions of TNF receptor superfamily members to CD8+ T-cell responses. Mol Syst Biol. 2021;17:e10560 pubmed publisher
	Geuijen C, Tacken P, Wang L, Klooster R, Van Loo P, Zhou J, et al. A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade. Nat Commun. 2021;12:4445 pubmed publisher
	Meckiff B, Ramírez Suástegui C, Fajardo V, Chee S, Kusnadi A, Simon H, et al. Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4+ T Cells in COVID-19. Cell. 2020;183:1340-1353.e16 pubmed publisher
	Bourges C, Groff A, Burren O, Gerhardinger C, Mattioli K, Hutchinson A, et al. Resolving mechanisms of immune-mediated disease in primary CD4 T cells. EMBO Mol Med. 2020;12:e12112 pubmed publisher
	Franchini D, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, et al. Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells. Cell Rep. 2019;26:94-107.e7 pubmed publisher
	Goletz C, Lischke T, Harnack U, Schiele P, Danielczyk A, Rühmann J, et al. Glyco-Engineered Anti-Human Programmed Death-Ligand 1 Antibody Mediates Stronger CD8 T Cell Activation Than Its Normal Glycosylated and Non-Glycosylated Counterparts. Front Immunol. 2018;9:1614 pubmed publisher

product information

Antigen :

CD137

Apps. Abbrev. :

Cat # :

309823

Clone :

4B4-1

Item :

Alexa Fluor® 647 anti-human CD137 (4-1BB)

Isotype :

Mouse IgG1, κ

Other Names :

4-1BB, ILA, CD137, TNFRSF9

Size :

25 tests

Price (USD) :

147 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

A647

Immunogen :

Ectodomain of recombinant human 4-1BB fusion protein

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,4, inhibition of cytokine production^2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).;

more info or order :

BioLegend product webpage