product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human CD137 (4-1BB)
catalog :
309802
quantity :
100 μg
price :
131 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4B4-1
reactivity :
human
application :
ELISA, immunoprecipitation, flow cytometry, mass cytometry
more info or order :
citations: 22
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 3a
Pei Y, Xiang Z, Wen K, Tu C, Wang X, Zhang Y, et al. CD137 Costimulation Enhances the Antitumor Activity of Vγ9Vδ2-T Cells in IL-10-Mediated Immunosuppressive Tumor Microenvironment. Front Immunol. 2022;13:872122 pubmed publisher
  • flow cytometry; human
Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
  • flow cytometry; human; 3:50; loading ...; fig 3j
Park J, Botting R, Domínguez Conde C, Popescu D, Lavaert M, Kunz D, et al. A cell atlas of human thymic development defines T cell repertoire formation. Science. 2020;367: pubmed publisher
  • flow cytometry; human; fig 3g
Montel Hagen A, Seet C, Li S, Chick B, Zhu Y, Chang P, et al. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019;24:376-389.e8 pubmed publisher
  • flow cytometry; human; loading ...; fig s2b
Scheper W, Kelderman S, Fanchi L, Linnemann C, Bendle G, de Rooij M, et al. Low and variable tumor reactivity of the intratumoral TCR repertoire in human cancers. Nat Med. 2019;25:89-94 pubmed publisher
  • mass cytometry; human; loading ...; fig 2a
Chew V, Lai L, Pan L, Lim C, Li J, Ong R, et al. Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses. Proc Natl Acad Sci U S A. 2017;114:E5900-E5909 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Lepore M, Kalinichenko A, Calogero S, Kumar P, Paleja B, Schmaler M, et al. Functionally diverse human T cells recognize non-microbial antigens presented by MR1. elife. 2017;6: pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Gabanti E, Bruno F, Scaramuzzi L, Mangione F, Zelini P, Gerna G, et al. Predictive value of human cytomegalovirus (HCMV) T-cell response in the control of HCMV infection by seropositive solid-organ transplant recipients according to different assays and stimuli. New Microbiol. 2016;39:247-258 pubmed
  • flow cytometry; human; fig 6
Jutz S, Leitner J, Schmetterer K, Doel Perez I, Majdic O, Grabmeier Pfistershammer K, et al. Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1. J Immunol Methods. 2016;430:10-20 pubmed publisher
Gadalla R, Boukhaled G, Brooks D, Wang B. Mass cytometry immunostaining protocol for multiplexing clinical samples. STAR Protoc. 2022;3:101643 pubmed publisher
Sun R, Kim A, Murray A, Lim S. N-Glycosylation Facilitates 4-1BB Membrane Localization by Avoiding Its Multimerization. Cells. 2022;11: pubmed publisher
Lu X, Hosono Y, Nagae M, Ishizuka S, Ishikawa E, Motooka D, et al. Identification of conserved SARS-CoV-2 spike epitopes that expand public cTfh clonotypes in mild COVID-19 patients. J Exp Med. 2021;218: pubmed publisher
Heesters B, van Megesen K, Tomris I, de Vries R, Magri G, Spits H. Characterization of human FDCs reveals regulation of T cells and antigen presentation to B cells. J Exp Med. 2021;218: pubmed publisher
Loo Yau H, Bell E, Ettayebi I, de Almeida F, Boukhaled G, Shen S, et al. DNA hypomethylating agents increase activation and cytolytic activity of CD8+ T cells. Mol Cell. 2021;81:1469-1483.e8 pubmed publisher
Law J, Koh W, Budylowski P, Lin J, Yue F, Abe K, et al. Systematic Examination of Antigen-Specific Recall T Cell Responses to SARS-CoV-2 versus Influenza Virus Reveals a Distinct Inflammatory Profile. J Immunol. 2021;206:37-50 pubmed publisher
Rodriguez L, Pekkarinen P, Lakshmikanth T, Tan Z, Consiglio C, Pou C, et al. Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19. Cell Rep Med. 2020;1:100078 pubmed publisher
Tang J, Compton B, Marshall A, Anderson R, Li Y, van der Woude H, et al. Mānuka honey-derived methylglyoxal enhances microbial sensing by mucosal-associated invariant T cells. Food Funct. 2020;11:5782-5787 pubmed publisher
Liu F, Liu W, Zhou S, Yang C, Tian M, Jia G, et al. Identification of FABP5 as an immunometabolic marker in human hepatocellular carcinoma. J Immunother Cancer. 2020;8: pubmed publisher
Friebel E, Kapolou K, Unger S, Núñez N, Utz S, Rushing E, et al. Single-Cell Mapping of Human Brain Cancer Reveals Tumor-Specific Instruction of Tissue-Invading Leukocytes. Cell. 2020;181:1626-1642.e20 pubmed publisher
Ichikawa J, Yoshida T, Isser A, Laino A, Vassallo M, Woods D, et al. Rapid Expansion of Highly Functional Antigen-Specific T Cells from Patients with Melanoma by Nanoscale Artificial Antigen-Presenting Cells. Clin Cancer Res. 2020;26:3384-3396 pubmed publisher
Speir M, Authier Hall A, Brooks C, Farrand K, Compton B, Anderson R, et al. Glycolipid-peptide conjugate vaccines enhance CD8+ T cell responses against human viral proteins. Sci Rep. 2017;7:14273 pubmed publisher
Lavin Y, Kobayashi S, Leader A, Amir E, Elefant N, Bigenwald C, et al. Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses. Cell. 2017;169:750-765.e17 pubmed publisher
product information
Antigen :
CD137
Apps. Abbrev. :
FC, IP, ELISA
Cat # :
309802
Clone :
4B4-1
Item :
Purified anti-human CD137 (4-1BB)
Isotype :
Mouse IgG1, κ
Other Names :
4-1BB, ILA, CD137, TNFRSF9
Size :
100 μg
Price (USD) :
131 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Ectodomain of recombinant human 4-1BB fusion protein
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation^1,4, inhibition of cytokine production^2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA