Alexa Fluor® 594 anti-human Perforin | BioLegend 308124 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Alexa Fluor® 594 anti-human Perforin

catalog :

308124

quantity :

100 μg

price :

288 USD

clonality :

monoclonal

host :

mouse

conjugate :

Alexa Fluor 594

clone name :

dG9

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, flow cytometry, other

more info or order :

BioLegend product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 2a	Chitirala P, Chang H, Martzloff P, Harenberg C, Ravichandran K, Abdulreda M, et al. Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse. elife. 2020;9: pubmed publisher
flow cytometry; human; 1:50; loading ...; fig 2b immunocytochemistry; human; loading ...; fig 2a	Zurli V, Montecchi T, Heilig R, Poschke I, Volkmar M, Wimmer G, et al. Phosphoproteomics of CD2 signaling reveals AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells. Sci Signal. 2020;13: pubmed publisher
flow cytometry; human; loading ...; fig 2a	Ingegnere T, Mariotti F, Pelosi A, Quintarelli C, De Angelis B, Tumino N, et al. Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing. Front Immunol. 2019;10:957 pubmed publisher
flow cytometry; human; loading ...; fig 1j	Ye W, Chew M, Hou J, Lai F, Leopold S, Loo H, et al. Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway. PLoS Pathog. 2018;14:e1007298 pubmed publisher
other; human; loading ...; fig 1b	Srpan K, Ambrose A, Karampatzakis A, Saeed M, Cartwright A, Guldevall K, et al. Shedding of CD16 disassembles the NK cell immune synapse and boosts serial engagement of target cells. J Cell Biol. 2018;217:3267-3283 pubmed publisher
flow cytometry; human; loading ...; fig 3a	Cheuk S, Schlums H, Gallais Sérézal I, Martini E, Chiang S, Marquardt N, et al. CD49a Expression Defines Tissue-Resident CD8+ T Cells Poised for Cytotoxic Function in Human Skin. Immunity. 2017;46:287-300 pubmed publisher
flow cytometry; human; 5 ug/ml; fig 5	Khazen R, MÃ¼ller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S. Melanoma cell lysosome secretory burst neutralizes the CTL-mediated cytotoxicity at the lytic synapse. Nat Commun. 2016;7:10823 pubmed publisher
immunocytochemistry; human; fig 8	Zhang Z, Wu N, Lu Y, Davidson D, Colonna M, Veillette A. DNAM-1 controls NK cell activation via an ITT-like motif. J Exp Med. 2015;212:2165-82 pubmed publisher
flow cytometry; human; 2,500 ug/ml; fig 3	Zhang P, Lu X, Tao K, Shi L, Li W, Wang G, et al. Siglec-10 is associated with survival and natural killer cell dysfunction in hepatocellular carcinoma. J Surg Res. 2015;194:107-13 pubmed publisher
flow cytometry; human; fig 4	Ziblat A, Domaica C, Spallanzani R, Iraolagoitia X, Rossi L, Avila D, et al. IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. Eur J Immunol. 2015;45:192-202 pubmed publisher
	Zander R, SCHAUDER D, Xin G, Nguyen C, Wu X, ZAJAC A, et al. CD4+ T Cell Help Is Required for the Formation of a Cytolytic CD8+ T Cell Subset that Protects against Chronic Infection and Cancer. Immunity. 2019;51:1028-1042.e4 pubmed publisher
	Wood S, Meeths M, Chiang S, Bechensteen A, Boelens J, Heilmann C, et al. Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity. Blood. 2009;114:4117-27 pubmed publisher

product information

Antigen :

Perforin

Apps. Abbrev. :

ICC

Cat # :

308124

Clone :

dG9

Item :

Alexa Fluor® 594 anti-human Perforin

Isotype :

Mouse IgG2b, κ

Other Names :

PRF1, P1, PFP, HPLH2

Size :

100 μg

Price (USD) :

288 USD

Reactivity :

Human

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

A594

Immunogen :

Purified granules from the human lymphoma cell line

Application Notes :

Clone dG9 primarily recognizes perforin associated with cytotoxic granules^9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy^5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^1,4.;Does not cross-react with mouse^1.;

more info or order :

BioLegend product webpage