product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-human Perforin
catalog :
308108
quantity :
100 tests
price :
265 USD
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
dG9
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, other
more info or order :
citations: 18
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 2a
Chitirala P, Chang H, Martzloff P, Harenberg C, Ravichandran K, Abdulreda M, et al. Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse. elife. 2020;9: pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; fig 4a, 7a
Gunesch J, Dixon A, Ebrahim T, Berrien Elliott M, Tatineni S, Kumar T, et al. CD56 regulates human NK cell cytotoxicity through Pyk2. elife. 2020;9: pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig 2b
  • immunocytochemistry; human; loading ...; fig 2a
Zurli V, Montecchi T, Heilig R, Poschke I, Volkmar M, Wimmer G, et al. Phosphoproteomics of CD2 signaling reveals AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells. Sci Signal. 2020;13: pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Ingegnere T, Mariotti F, Pelosi A, Quintarelli C, De Angelis B, Tumino N, et al. Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing. Front Immunol. 2019;10:957 pubmed publisher
  • flow cytometry; human; loading ...; fig 1j
Ye W, Chew M, Hou J, Lai F, Leopold S, Loo H, et al. Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway. PLoS Pathog. 2018;14:e1007298 pubmed publisher
  • other; human; loading ...; fig 1b
Srpan K, Ambrose A, Karampatzakis A, Saeed M, Cartwright A, Guldevall K, et al. Shedding of CD16 disassembles the NK cell immune synapse and boosts serial engagement of target cells. J Cell Biol. 2018;217:3267-3283 pubmed publisher
  • flow cytometry; human; 5 ug/ml; fig 5
Khazen R, Müller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S. Melanoma cell lysosome secretory burst neutralizes the CTL-mediated cytotoxicity at the lytic synapse. Nat Commun. 2016;7:10823 pubmed publisher
  • flow cytometry; human; fig 4
Ziblat A, Domaica C, Spallanzani R, Iraolagoitia X, Rossi L, Avila D, et al. IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. Eur J Immunol. 2015;45:192-202 pubmed publisher
Kennedy P, Vallera D, Ettestad B, Hallstrom C, Kodal B, Todhunter D, et al. A tri-specific killer engager against mesothelin targets NK cells towards lung cancer. Front Immunol. 2023;14:1060905 pubmed publisher
Oyer J, Croom Perez T, Dieffenthaller T, Robles Carillo L, Gitto S, Altomare D, et al. Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo. Front Immunol. 2022;13:861681 pubmed publisher
Thieme C, Anft M, Paniskaki K, Blazquez Navarro A, Doevelaar A, Seibert F, et al. Robust T Cell Response Toward Spike, Membrane, and Nucleocapsid SARS-CoV-2 Proteins Is Not Associated with Recovery in Critical COVID-19 Patients. Cell Rep Med. 2020;1:100092 pubmed publisher
Ambrose A, Hazime K, Worboys J, Niembro Vivanco O, Davis D. Synaptic secretion from human natural killer cells is diverse and includes supramolecular attack particles. Proc Natl Acad Sci U S A. 2020;117:23717-23720 pubmed publisher
Carisey A, Mace E, Saeed M, Davis D, Orange J. Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells. Curr Biol. 2018;28:489-502.e9 pubmed publisher
Davey M, Willcox C, Joyce S, Ladell K, Kasatskaya S, McLaren J, et al. Clonal selection in the human Vδ1 T cell repertoire indicates γδ TCR-dependent adaptive immune surveillance. Nat Commun. 2017;8:14760 pubmed publisher
Salzer E, Cağdaş D, Hons M, Mace E, Garncarz W, Petronczki Ö, et al. RASGRP1 deficiency causes immunodeficiency with impaired cytoskeletal dynamics. Nat Immunol. 2016;17:1352-1360 pubmed publisher
Kabanova A, Sanseviero F, Candi V, Gamberucci A, Gozzetti A, Campoccia G, et al. Human Cytotoxic T Lymphocytes Form Dysfunctional Immune Synapses with B Cells Characterized by Non-Polarized Lytic Granule Release. Cell Rep. 2016;15:9-18 pubmed publisher
Mace E, Orange J. Lytic immune synapse function requires filamentous actin deconstruction by Coronin 1A. Proc Natl Acad Sci U S A. 2014;111:6708-13 pubmed publisher
Wood S, Meeths M, Chiang S, Bechensteen A, Boelens J, Heilmann C, et al. Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity. Blood. 2009;114:4117-27 pubmed publisher
product information
Antigen :
Perforin
Apps. Abbrev. :
ICFC
Cat # :
308108
Clone :
dG9
Item :
Alexa Fluor® 488 anti-human Perforin
Isotype :
Mouse IgG2b, κ
Other Names :
PRF1, P1, PFP, HPLH2
Size :
100 tests
Price (USD) :
265 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
A488
Immunogen :
Purified granules from the human lymphoma cell line
Application Notes :
Clone dG9 primarily recognizes perforin associated with cytotoxic granules^9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy^5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^1,4.;Does not cross-react with mouse^1.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA