product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-human Perforin
catalog :
308102
quantity :
100 μg
price :
206 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
dG9
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, other
more info or order :
citations: 24
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; loading ...; fig 2a
Chitirala P, Chang H, Martzloff P, Harenberg C, Ravichandran K, Abdulreda M, et al. Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse. elife. 2020;9: pubmed publisher
  • flow cytometry; human; 1:50; loading ...; fig 2b
  • immunocytochemistry; human; loading ...; fig 2a
Zurli V, Montecchi T, Heilig R, Poschke I, Volkmar M, Wimmer G, et al. Phosphoproteomics of CD2 signaling reveals AMPK-dependent regulation of lytic granule polarization in cytotoxic T cells. Sci Signal. 2020;13: pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Ingegnere T, Mariotti F, Pelosi A, Quintarelli C, De Angelis B, Tumino N, et al. Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing. Front Immunol. 2019;10:957 pubmed publisher
  • flow cytometry; human; loading ...; fig 1j
Ye W, Chew M, Hou J, Lai F, Leopold S, Loo H, et al. Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway. PLoS Pathog. 2018;14:e1007298 pubmed publisher
  • other; human; loading ...; fig 1b
Srpan K, Ambrose A, Karampatzakis A, Saeed M, Cartwright A, Guldevall K, et al. Shedding of CD16 disassembles the NK cell immune synapse and boosts serial engagement of target cells. J Cell Biol. 2018;217:3267-3283 pubmed publisher
  • flow cytometry; human; loading ...; fig 3a
Cheuk S, Schlums H, Gallais Sérézal I, Martini E, Chiang S, Marquardt N, et al. CD49a Expression Defines Tissue-Resident CD8+ T Cells Poised for Cytotoxic Function in Human Skin. Immunity. 2017;46:287-300 pubmed publisher
  • flow cytometry; human; fig 1e
Cuff A, Robertson F, Stegmann K, Pallett L, Maini M, Davidson B, et al. Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation. J Immunol. 2016;197:4283-4291 pubmed
  • flow cytometry; human; 5 ug/ml; fig 5
Khazen R, Müller S, Gaudenzio N, Espinosa E, Puissegur M, Valitutti S. Melanoma cell lysosome secretory burst neutralizes the CTL-mediated cytotoxicity at the lytic synapse. Nat Commun. 2016;7:10823 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Roan F, Stoklasek T, Whalen E, Molitor J, Bluestone J, Buckner J, et al. CD4+ Group 1 Innate Lymphoid Cells (ILC) Form a Functionally Distinct ILC Subset That Is Increased in Systemic Sclerosis. J Immunol. 2016;196:2051-2062 pubmed publisher
  • immunocytochemistry; human; fig 8
Zhang Z, Wu N, Lu Y, Davidson D, Colonna M, Veillette A. DNAM-1 controls NK cell activation via an ITT-like motif. J Exp Med. 2015;212:2165-82 pubmed publisher
  • flow cytometry; human
Rosario M, Liu B, Kong L, Collins L, Schneider S, Chen X, et al. The IL-15-Based ALT-803 Complex Enhances FcγRIIIa-Triggered NK Cell Responses and In Vivo Clearance of B Cell Lymphomas. Clin Cancer Res. 2016;22:596-608 pubmed publisher
  • flow cytometry; human; 2,500 ug/ml; fig 3
Zhang P, Lu X, Tao K, Shi L, Li W, Wang G, et al. Siglec-10 is associated with survival and natural killer cell dysfunction in hepatocellular carcinoma. J Surg Res. 2015;194:107-13 pubmed publisher
  • flow cytometry; human; fig 4
Ziblat A, Domaica C, Spallanzani R, Iraolagoitia X, Rossi L, Avila D, et al. IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. Eur J Immunol. 2015;45:192-202 pubmed publisher
  • immunocytochemistry; human
Peguillet I, Milder M, Louis D, Vincent Salomon A, Dorval T, Piperno Neumann S, et al. High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer. Cancer Res. 2014;74:2204-16 pubmed publisher
  • flow cytometry; human
Poonia B, Pauza C. Levels of CD56+TIM-3- effector CD8 T cells distinguish HIV natural virus suppressors from patients receiving antiretroviral therapy. PLoS ONE. 2014;9:e88884 pubmed publisher
  • flow cytometry; human
Radvanyi L, Bernatchez C, Zhang M, Fox P, Miller P, Chacon J, et al. Specific lymphocyte subsets predict response to adoptive cell therapy using expanded autologous tumor-infiltrating lymphocytes in metastatic melanoma patients. Clin Cancer Res. 2012;18:6758-70 pubmed publisher
Poon M, Caron D, Wang Z, Wells S, Chen D, Meng W, et al. Tissue adaptation and clonal segregation of human memory T cells in barrier sites. Nat Immunol. 2023;24:309-319 pubmed publisher
Trinks N, Reinhard S, Drobny M, Heilig L, L xf6 ffler J, Sauer M, et al. Subdiffraction-resolution fluorescence imaging of immunological synapse formation between NK cells and A. fumigatus by expansion microscopy. Commun Biol. 2021;4:1151 pubmed publisher
Mann E, Menon M, Knight S, Konkel J, Jagger C, Shaw T, et al. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19. Sci Immunol. 2020;5: pubmed publisher
Crespo A, Mulik S, Dotiwala F, Ansara J, Sen Santara S, Ingersoll K, et al. Decidual NK Cells Transfer Granulysin to Selectively Kill Bacteria in Trophoblasts. Cell. 2020;182:1125-1139.e18 pubmed publisher
Dean J, Peters L, Fuhrman C, Seay H, Posgai A, Stimpson S, et al. Innate inflammation drives NK cell activation to impair Treg activity. J Autoimmun. 2020;108:102417 pubmed publisher
Del Alcazar D, Wang Y, He C, Wendel B, Del Rio Estrada P, Lin J, et al. Mapping the Lineage Relationship between CXCR5+ and CXCR5- CD4+ T Cells in HIV-Infected Human Lymph Nodes. Cell Rep. 2019;28:3047-3060.e7 pubmed publisher
Mace E, Orange J. Visualization of the immunological synapse by dual color time-gated stimulated emission depletion (STED) nanoscopy. J Vis Exp. 2014;: pubmed publisher
Wood S, Meeths M, Chiang S, Bechensteen A, Boelens J, Heilmann C, et al. Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity. Blood. 2009;114:4117-27 pubmed publisher
product information
Antigen :
Perforin
Apps. Abbrev. :
ICFC, IP, ICC, IHC
Cat # :
308102
Clone :
dG9
Item :
Purified anti-human Perforin
Isotype :
Mouse IgG2b, κ
Other Names :
PRF1, P1, PFP, HPLH2
Size :
100 μg
Price (USD) :
206 USD
Reactivity :
Human
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PURE
Immunogen :
Purified granules from the human lymphoma cell line
Application Notes :
Clone dG9 primarily recognizes perforin associated with cytotoxic granules^9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy^5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^1,4.;Does not cross-react with mouse^1.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA