TotalSeq™-C0159 anti-human HLA-DR | BioLegend 307663 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

TotalSeq™-C0159 anti-human HLA-DR

catalog :

307663

quantity :

10 μg

price :

369 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

L243

reactivity :

human, mouse, rhesus macaque

application :

flow cytometry, mass cytometry

more info or order :

BioLegend product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...	Amaral E, Foreman T, Namasivayam S, Hilligan K, Kauffman K, Barbosa Bomfim C, et al. GPX4 regulates cellular necrosis and host resistance in Mycobacterium tuberculosis infection. J Exp Med. 2022;219: pubmed publisher
flow cytometry; human; loading ...; fig e9a	Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
flow cytometry; human; 1:300; loading ...; fig 6b	Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
flow cytometry; human; loading ...; fig 3b	Ingelfinger F, Krishnarajah S, Kramer M, Utz S, Galli E, Lutz M, et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature. Acta Neuropathol. 2021;141:901-915 pubmed publisher
flow cytometry; human	Chiou S, Tseng D, Reuben A, Mallajosyula V, Molina I, Conley S, et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity. 2021;54:586-602.e8 pubmed publisher
flow cytometry; human; loading ...; fig 3a	Gregorova M, Morse D, Brignoli T, Steventon J, Hamilton F, Albur M, et al. Post-acute COVID-19 associated with evidence of bystander T-cell activation and a recurring antibiotic-resistant bacterial pneumonia. elife. 2020;9: pubmed publisher
flow cytometry; human; loading ...; fig 1b	Stary V, Wolf B, Unterleuthner D, List J, Talic M, Laengle J, et al. Short-course radiotherapy promotes pro-inflammatory macrophages via extracellular vesicles in human rectal cancer. J Immunother Cancer. 2020;8: pubmed publisher
flow cytometry; human; loading ...; fig s1c	Morrissey M, Byrne R, Nulty C, McCabe N, Lynam Lennon N, Butler C, et al. The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation. BMC Cancer. 2020;20:566 pubmed publisher
flow cytometry; human; 3:50; loading ...; fig 1c	Park J, Botting R, Domínguez Conde C, Popescu D, Lavaert M, Kunz D, et al. A cell atlas of human thymic development defines T cell repertoire formation. Science. 2020;367: pubmed publisher
mass cytometry; human; 500 ug/ml; loading ...; fig s11a	Helmink B, Reddy S, Gao J, Zhang S, Basar R, Thakur R, et al. B cells and tertiary lymphoid structures promote immunotherapy response. Nature. 2020;577:549-555 pubmed publisher
mass cytometry; human; loading ...; fig 1b, 1d, s2	Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
flow cytometry; human; loading ...; fig s6c	Stewart B, Ferdinand J, Young M, Mitchell T, Loudon K, Riding A, et al. Spatiotemporal immune zonation of the human kidney. Science. 2019;365:1461-1466 pubmed publisher
flow cytometry; human; loading ...	Merola J, Reschke M, Pierce R, Qin L, Spindler S, Baltazar T, et al. Progenitor-derived human endothelial cells evade alloimmunity by CRISPR/Cas9-mediated complete ablation of MHC expression. JCI Insight. 2019;4: pubmed publisher
flow cytometry; human; loading ...; fig 8a	Saliba D, Céspedes Donoso P, Balint S, Compeer E, Korobchevskaya K, Valvo S, et al. Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells. elife. 2019;8: pubmed publisher
flow cytometry; human; loading ...; fig s1	Swaims Kohlmeier A, Haddad L, Li Z, Brookmeyer K, Baker J, Widom C, et al. Chronic immune barrier dysregulation among women with a history of violence victimization. JCI Insight. 2019;4: pubmed publisher
mass cytometry; human; loading ...; fig s1	Fernandez I, Baxter R, Garcia Perez J, Vendrame E, Ranganath T, Kong D, et al. A novel human IL2RB mutation results in T and NK cell-driven immune dysregulation. J Exp Med. 2019;216:1255-1267 pubmed publisher
flow cytometry; human; loading ...; fig s1b	Zhang J, Supakorndej T, Krambs J, Rao M, Abou Ezzi G, Ye R, et al. Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking. J Clin Invest. 2019;129:2920-2931 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Terahara K, Iwabuchi R, Hosokawa M, Nishikawa Y, Takeyama H, Takahashi Y, et al. A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro. BMC Res Notes. 2019;12:242 pubmed publisher
flow cytometry; human; 1:200; loading ...; fig 1b	Jones G, Bain C, Fenton T, Kelly A, Brown S, Ivens A, et al. Dynamics of Colon Monocyte and Macrophage Activation During Colitis. Front Immunol. 2018;9:2764 pubmed publisher
flow cytometry; mouse; loading ...; fig 3b	Poncette L, Chen X, Lorenz F, Blankenstein T. Effective NY-ESO-1-specific MHC II-restricted T cell receptors from antigen-negative hosts enhance tumor regression. J Clin Invest. 2019;129:324-335 pubmed publisher
flow cytometry; human; loading ...; fig s1	Melo Gonzalez F, Fenton T, Forss C, Smedley C, Goenka A, MacDonald A, et al. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner. J Biol Chem. 2018;293:8543-8553 pubmed publisher
	Xu Q, Milanez Almeida P, Martins A, Radtke A, Hoehn K, Oguz C, et al. Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children. Nat Immunol. 2023;24:186-199 pubmed publisher
	Xu C, Yang J, Kosters A, Babcock B, Qiu P, Ghosn E. Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system. iScience. 2022;25:105123 pubmed publisher
	Li S, Hickey A, Shangguan S, Ehrenberg P, Geretz A, Butler L, et al. HLA-B∗46 associates with rapid HIV disease progression in Asian cohorts and prominent differences in NK cell phenotype. Cell Host Microbe. 2022;30:1173-1185.e8 pubmed publisher
	Collora J, Liu R, Pinto Santini D, Ravindra N, Ganoza C, Lama J, et al. Single-cell multiomics reveals persistence of HIV-1 in expanded cytotoxic T cell clones. Immunity. 2022;55:1013-1031.e7 pubmed publisher
	Bachireddy P, Azizi E, Burdziak C, Nguyen V, Ennis C, Maurer K, et al. Mapping the evolution of T cell states during response and resistance to adoptive cellular therapy. Cell Rep. 2021;37:109992 pubmed publisher
	Shangguan S, Ehrenberg P, Geretz A, Yum L, Kundu G, May K, et al. Monocyte-derived transcriptome signature indicates antibody-dependent cellular phagocytosis as a potential mechanism of vaccine-induced protection against HIV-1. elife. 2021;10: pubmed publisher
	Ferreira Gomes M, Kruglov A, Durek P, Heinrich F, Tizian C, Heinz G, et al. SARS-CoV-2 in severe COVID-19 induces a TGF-β-dominated chronic immune response that does not target itself. Nat Commun. 2021;12:1961 pubmed publisher
	Sokal A, Chappert P, Barba Spaeth G, Roeser A, Fourati S, Azzaoui I, et al. Maturation and persistence of the anti-SARS-CoV-2 memory B cell response. Cell. 2021;184:1201-1213.e14 pubmed publisher
	Remmerie A, Martens L, Thoné T, Castoldi A, Seurinck R, Pavie B, et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver. Immunity. 2020;53:641-657.e14 pubmed publisher

product information

Antigen :

HLA-DR

Apps. Abbrev. :

Cat # :

307663

Clone :

L243

Item :

TotalSeq™-C0159 anti-human HLA-DR

Isotype :

Mouse IgG2a, κ

Other Names :

Major Histocompatibility Class II, MHC class II

Size :

10 μg

Price (USD) :

369 USD

Reactivity :

Human, Cynomolgus, Rhesus

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

TOTALSEQ_C

Application Notes :

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.^19Additional reported applications (for the relevant formats) include: immunoprecipitation^8, Western blotting^8, in vitro blocking of mixed lymphocyte reactions^9,10, depeletion of MHC class II cells^7, immunohistochemical staining of acetone-fixed frozen sections^4,5, and spatial biology (IBEX)^21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).;

more info or order :

BioLegend product webpage