product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 650™ anti-human HLA-DR
catalog :
307649
quantity :
25 tests
price :
215 USD
clonality :
monoclonal
host :
mouse
conjugate :
BV650
clone name :
L243
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 21
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1a
Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Izmirly A, Pelletier A, Connors J, Taramangalam B, Alturki S, Gordon E, et al. Pre-vaccination frequency of circulatory Tfh is associated with robust immune response to TV003 dengue vaccine. PLoS Pathog. 2022;18:e1009903 pubmed publisher
Chakraborty S, González J, Sievers B, Mallajosyula V, Chakraborty S, Dubey M, et al. Early non-neutralizing, afucosylated antibody responses are associated with COVID-19 severity. Sci Transl Med. 2022;14:eabm7853 pubmed publisher
Obradovic A, Chowdhury N, Haake S, Ager C, Wang V, Vlahos L, et al. Single-cell protein activity analysis identifies recurrence-associated renal tumor macrophages. Cell. 2021;184:2988-3005.e16 pubmed publisher
Hoang T, Pino M, Boddapati A, Viox E, Starke C, Upadhyay A, et al. Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques. Cell. 2021;184:460-475.e21 pubmed publisher
Dhariwala M, Karthikeyan D, Vasquez K, Farhat S, Weckel A, Taravati K, et al. Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature. Cell Rep Med. 2020;1:100132 pubmed publisher
Ott P, Hu Lieskovan S, Chmielowski B, Govindan R, Naing A, Bhardwaj N, et al. A Phase Ib Trial of Personalized Neoantigen Therapy Plus Anti-PD-1 in Patients with Advanced Melanoma, Non-small Cell Lung Cancer, or Bladder Cancer. Cell. 2020;183:347-362.e24 pubmed publisher
Liang J, Wang H, Ding W, Huang J, Zhou X, Wang H, et al. Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity. Sci Adv. 2020;6:eabc3646 pubmed publisher
Asokan M, Dias J, Liu C, Maximova A, Ernste K, Pegu A, et al. Fc-mediated effector function contributes to the in vivo antiviral effect of an HIV neutralizing antibody. Proc Natl Acad Sci U S A. 2020;117:18754-18763 pubmed publisher
Utz S, See P, Mildenberger W, Thion M, Silvin A, Lutz M, et al. Early Fate Defines Microglia and Non-parenchymal Brain Macrophage Development. Cell. 2020;181:557-573.e18 pubmed publisher
Cassetta L, Fragkogianni S, Sims A, Swierczak A, Forrester L, Zhang H, et al. Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell. 2019;35:588-602.e10 pubmed publisher
Kumar M, Du J, Lagoudas G, Jiao Y, Sawyer A, Drummond D, et al. Analysis of Single-Cell RNA-Seq Identifies Cell-Cell Communication Associated with Tumor Characteristics. Cell Rep. 2018;25:1458-1468.e4 pubmed publisher
Yarzabek B, Zaitouna A, Olson E, Silva G, Geng J, Geretz A, et al. Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity. elife. 2018;7: pubmed publisher
McCracken M, Gromowski G, Friberg H, Lin X, Abbink P, De La Barrera R, et al. Impact of prior flavivirus immunity on Zika virus infection in rhesus macaques. PLoS Pathog. 2017;13:e1006487 pubmed publisher
Ries M, Reynolds M, Bashkueva K, Crosno K, Capuano S, Prall T, et al. KIR3DL01 upregulation on gut natural killer cells in response to SIV infection of KIR- and MHC class I-defined rhesus macaques. PLoS Pathog. 2017;13:e1006506 pubmed publisher
Lauterbach N, Crivello P, Wieten L, Zito L, Groeneweg M, Voorter C, et al. Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain. Mol Immunol. 2014;59:19-29 pubmed publisher
Gonçalves R, Salmazi K, Santos B, Bastos M, Rocha S, Boscardin S, et al. CD4+ CD25+ Foxp3+ regulatory T cells, dendritic cells, and circulating cytokines in uncomplicated malaria: do different parasite species elicit similar host responses?. Infect Immun. 2010;78:4763-72 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Fujita H, Nograles K, Kikuchi T, Gonzalez J, Carucci J, Krueger J. Human Langerhans cells induce distinct IL-22-producing CD4+ T cells lacking IL-17 production. Proc Natl Acad Sci U S A. 2009;106:21795-800 pubmed publisher
Zaba L, Cardinale I, Gilleaudeau P, Sullivan Whalen M, Suárez Fariñas M, Fuentes Duculan J, et al. Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses. J Exp Med. 2007;204:3183-94 pubmed
product information
Antigen :
HLA-DR
Apps. Abbrev. :
Flow Cytometry
Cat # :
307649
Clone :
L243
Item :
Brilliant Violet 650™ anti-human HLA-DR
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class II, MHC class II
Size :
25 tests
Price (USD) :
215 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
BV650
Application Notes :
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a heterodimer. 19 Additional reported applications (for the relevant formats) include: immunoprecipitation 8 , Western blotting 8 , in vitro blocking of mixed lymphocyte reactions 9,10 , depeletion of MHC class II cells 7 , immunohistochemical staining of acetone-fixed frozen sections 4,5 , and spatial biology (IBEX) 21,22 . For sensitive functional assays, we recommend using the Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 307648, 307665 - 307669).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA