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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-human HLA-DR
catalog :
307632
quantity :
100 μg
price :
147 USD
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
L243
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 32
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:200; loading ...; fig s1d
Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, et al. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement. Nat Commun. 2017;8:14253 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
  • flow cytometry; human; loading ...; fig 7a
Zanetti S, Ziblat A, Torres N, Zwirner N, Bouzat C. Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells. J Biol Chem. 2016;291:16541-52 pubmed publisher
  • flow cytometry; human; fig 3
Rogacev K, Zawada A, Hundsdorfer J, Achenbach M, Held G, Fliser D, et al. Immunosuppression and monocyte subsets. Nephrol Dial Transplant. 2015;30:143-53 pubmed publisher
  • flow cytometry; human
Chao Y, Kaliaperumal N, Chretien A, Tang S, Lee B, Poidinger M, et al. Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα. J Leukoc Biol. 2014;96:1037-46 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Walter F, Winter E, Rahn S, Heidland J, Meier S, Struzek A, et al. Chitosan nanoparticles as antigen vehicles to induce effective tumor specific T cell responses. PLoS ONE. 2020;15:e0239369 pubmed publisher
He Z, Li W, Zheng T, Liu D, Zhao S. Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression. J Exp Clin Cancer Res. 2020;39:140 pubmed publisher
Tu S, Wu J, Chen L, Tian Y, Qin W, Huang S, et al. LncRNA CALB2 sponges miR-30b-3p to promote odontoblast differentiation of human dental pulp stem cells via up-regulating RUNX2. Cell Signal. 2020;73:109695 pubmed publisher
Tang X, Zhang S, Peng Q, Ling L, Shi H, Liu Y, et al. Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection. Sci Adv. 2020;6:eaaz0374 pubmed publisher
Manukyan G, Martirosyan A, Slavik L, Margaryan S, Ulehlova J, Mikulkova Z, et al. Anti-domain 1 β2 glycoprotein antibodies increase expression of tissue factor on monocytes and activate NK Cells and CD8+ cells in vitro. Auto Immun Highlights. 2020;11:5 pubmed publisher
Wang C, Park J, Ouyang C, Longmate J, Tajon M, Chao J, et al. A Pilot Feasibility Study of Yttrium-90 Liver Radioembolization Followed by Durvalumab and Tremelimumab in Patients with Microsatellite Stable Colorectal Cancer Liver Metastases. Oncologist. 2020;25:382-e776 pubmed publisher
Oshchepkova A, Neumestova A, Matveeva V, Artemyeva L, Morozova K, Kiseleva E, et al. Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer. Micromachines (Basel). 2019;10: pubmed publisher
Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
Xia Y, Gao Y, Wang B, Zhang H, Zhang Q. Optimizing the Method of Cell Separation from Bile of Patients with Cholangiocarcinoma for Flow Cytometry. Gastroenterol Res Pract. 2019;2019:5436961 pubmed publisher
Rochat M, Schlaepfer E, Kuster S, Li D, Audigé A, Ivic S, et al. Monitoring HIV DNA and cellular activation markers in HIV-infected humanized mice under cART. Virol J. 2018;15:191 pubmed publisher
Wang B, Lin Y, Hu Y, Shan W, Liu S, Xu Y, et al. mTOR inhibition improves the immunomodulatory properties of human bone marrow mesenchymal stem cells by inducing COX-2 and PGE2. Stem Cell Res Ther. 2017;8:292 pubmed publisher
Zhou Y, Du J, Hou H, Lu Y, Yu J, Mao L, et al. Application of ImmunoScore Model for the Differentiation between Active Tuberculosis and Latent Tuberculosis Infection as Well as Monitoring Anti-tuberculosis Therapy. Front Cell Infect Microbiol. 2017;7:457 pubmed publisher
Fang W, Douglas I, Chen Y, Lin C, Kao H, Fang Y, et al. Development and validation of immune dysfunction score to predict 28-day mortality of sepsis patients. PLoS ONE. 2017;12:e0187088 pubmed publisher
Shan L, Deng K, Gao H, Xing S, Capoferri A, Durand C, et al. Transcriptional Reprogramming during Effector-to-Memory Transition Renders CD4+ T Cells Permissive for Latent HIV-1 Infection. Immunity. 2017;47:766-775.e3 pubmed publisher
Neuser J, Galuppo P, Fraccarollo D, Willig J, Kempf T, Berliner D, et al. Intermediate CD14++CD16+ monocytes decline after transcatheter aortic valve replacement and correlate with functional capacity and left ventricular systolic function. PLoS ONE. 2017;12:e0183670 pubmed publisher
Muenchhoff M, Adland E, Karimanzira O, Crowther C, Pace M, Csala A, et al. Nonprogressing HIV-infected children share fundamental immunological features of nonpathogenic SIV infection. Sci Transl Med. 2016;8:358ra125 pubmed publisher
Santiago K, Conti B, Cardoso E, Golim M, Sforcin J. Immunomodulatory/anti-inflammatory effects of a propolis-containing mouthwash on human monocytes. Pathog Dis. 2016;74: pubmed
Sade Feldman M, Kanterman J, Klieger Y, Ish Shalom E, Olga M, Saragovi A, et al. Clinical Significance of Circulating CD33+CD11b+HLA-DR- Myeloid Cells in Patients with Stage IV Melanoma Treated with Ipilimumab. Clin Cancer Res. 2016;22:5661-5672 pubmed
Serrano Villar S, Sainz T, Ma Z, Utay N, Chun T, Wook Chun T, et al. Effects of Combined CCR5/Integrase Inhibitors-Based Regimen on Mucosal Immunity in HIV-Infected Patients Naïve to Antiretroviral Therapy: A Pilot Randomized Trial. PLoS Pathog. 2016;12:e1005381 pubmed publisher
Collison J, Carlin L, Eichmann M, Geissmann F, Peakman M. Heterogeneity in the Locomotory Behavior of Human Monocyte Subsets over Human Vascular Endothelium In Vitro. J Immunol. 2015;195:1162-70 pubmed publisher
Lathrop S, Binder K, Starr T, Cooper K, Chong A, Carmody A, et al. Replication of Salmonella enterica Serovar Typhimurium in Human Monocyte-Derived Macrophages. Infect Immun. 2015;83:2661-71 pubmed publisher
Planès R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E. HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms. J Virol. 2014;88:6672-89 pubmed publisher
Lauterbach N, Crivello P, Wieten L, Zito L, Groeneweg M, Voorter C, et al. Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain. Mol Immunol. 2014;59:19-29 pubmed publisher
Hsieh J, Huang T, Cheng S, Lin W, Tsai T, Lee O, et al. miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells. Nucleic Acids Res. 2013;41:9753-63 pubmed publisher
Wang X, Barnes P, Huang F, Alvarez I, Neuenschwander P, Sherman D, et al. Early secreted antigenic target of 6-kDa protein of Mycobacterium tuberculosis primes dendritic cells to stimulate Th17 and inhibit Th1 immune responses. J Immunol. 2012;189:3092-103 pubmed publisher
Iida H, Asada H, Yokoi S, Niizeki H, Yasuda Y, Miyagawa S, et al. Regulatory effects of antihistamines on the responses to staphylococcal enterotoxin B of human monocyte-derived dendritic cells and CD4+ T cells. J Dermatol Sci. 2008;52:31-8 pubmed publisher
product information
Antigen :
HLA-DR
Apps. Abbrev. :
Flow Cytometry
Cat # :
307632
Clone :
L243
Item :
FITC anti-human HLA-DR
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class II, MHC class II
Size :
100 μg
Price (USD) :
147 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
FITC
Application Notes :
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a heterodimer. 19 Additional reported applications (for the relevant formats) include: immunoprecipitation 8 , Western blotting 8 , in vitro blocking of mixed lymphocyte reactions 9,10 , depeletion of MHC class II cells 7 , immunohistochemical staining of acetone-fixed frozen sections 4,5 , and spatial biology (IBEX) 21,22 . For sensitive functional assays, we recommend using the Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 307648, 307665 - 307669).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA