product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-human HLA-DR
catalog :
307618
quantity :
100 tests
price :
292 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC/Cy7
clone name :
L243
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 53
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:300; loading ...; fig 6b
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; human; loading ...; fig s1c
Morrissey M, Byrne R, Nulty C, McCabe N, Lynam Lennon N, Butler C, et al. The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation. BMC Cancer. 2020;20:566 pubmed publisher
  • flow cytometry; human; 1:200; loading ...; fig 1b
Jones G, Bain C, Fenton T, Kelly A, Brown S, Ivens A, et al. Dynamics of Colon Monocyte and Macrophage Activation During Colitis. Front Immunol. 2018;9:2764 pubmed publisher
  • flow cytometry; human; loading ...; fig s3k
Ding L, Kim H, Wang Q, Kearns M, Jiang T, Ohlson C, et al. PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018;25:2972-2980.e5 pubmed publisher
  • flow cytometry; human; fig 4a
Kelly A, Günaltay S, McEntee C, Shuttleworth E, Smedley C, Houston S, et al. Human monocytes and macrophages regulate immune tolerance via integrin αvβ8-mediated TGFβ activation. J Exp Med. 2018;215:2725-2736 pubmed publisher
  • flow cytometry; human
Hautefort A, Girerd B, Montani D, Cohen Kaminsky S, Price L, Lambrecht B, et al. T-helper 17 cell polarization in pulmonary arterial hypertension. Chest. 2015;147:1610-1620 pubmed publisher
  • flow cytometry; human
Jitschin R, Braun M, Büttner M, Dettmer Wilde K, Bricks J, Berger J, et al. CLL-cells induce IDOhi CD14+HLA-DRlo myeloid-derived suppressor cells that inhibit T-cell responses and promote TRegs. Blood. 2014;124:750-60 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell. 2022;185:916-938.e58 pubmed publisher
Schimek V, Strasser K, Beer A, Göber S, Walterskirchen N, Brostjan C, et al. Tumour cell apoptosis modulates the colorectal cancer immune microenvironment via interleukin-8-dependent neutrophil recruitment. Cell Death Dis. 2022;13:113 pubmed publisher
Pesce E, Manfrini N, Cordiglieri C, Santi S, Bandera A, Gobbini A, et al. Exosomes Recovered From the Plasma of COVID-19 Patients Expose SARS-CoV-2 Spike-Derived Fragments and Contribute to the Adaptive Immune Response. Front Immunol. 2021;12:785941 pubmed publisher
Lewis S, Sureshchandra S, Doratt B, Jimenez V, Stull C, Grant K, et al. Transcriptional, Epigenetic, and Functional Reprogramming of Monocytes From Non-Human Primates Following Chronic Alcohol Drinking. Front Immunol. 2021;12:724015 pubmed publisher
Savage A, Gutschow M, Chiang T, Henderson K, Green R, Chaudhari M, et al. Multimodal analysis for human ex vivo studies shows extensive molecular changes from delays in blood processing. iScience. 2021;24:102404 pubmed publisher
Vetsika E, Sharma P, Samaras I, Markou A, Georgoulias V, Whiteside T, et al. Small Extracellular Vesicles in Pre-Therapy Plasma Predict Clinical Outcome in Non-Small-Cell Lung Cancer Patients. Cancers (Basel). 2021;13: pubmed publisher
Takiguchi H, Yang C, Yang C, Sahin B, Whalen B, Milne S, et al. Macrophages with reduced expressions of classical M1 and M2 surface markers in human bronchoalveolar lavage fluid exhibit pro-inflammatory gene signatures. Sci Rep. 2021;11:8282 pubmed publisher
Swanson E, Lord C, Reading J, Heubeck A, Genge P, Thomson Z, et al. Simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq. elife. 2021;10: pubmed publisher
Rodrigues E, Jung J, Park H, Loo C, Soukhtehzari S, Kitova E, et al. A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands. Nat Commun. 2020;11:5091 pubmed publisher
Tripathi H, Al Darraji A, Abo Aly M, Peng H, Shokri E, Chelvarajan L, et al. Autotaxin inhibition reduces cardiac inflammation and mitigates adverse cardiac remodeling after myocardial infarction. J Mol Cell Cardiol. 2020;149:95-114 pubmed publisher
Pribyl M, Hubackova S, Moudra A, Vancurova M, Polackova H, Stopka T, et al. Aberrantly elevated suprabasin in the bone marrow as a candidate biomarker of advanced disease state in myelodysplastic syndromes. Mol Oncol. 2020;14:2403-2419 pubmed publisher
Gorini G, Fourati S, Vaccari M, Rahman M, Gordon S, Brown D, et al. Engagement of monocytes, NK cells, and CD4+ Th1 cells by ALVAC-SIV vaccination results in a decreased risk of SIVmac251 vaginal acquisition. PLoS Pathog. 2020;16:e1008377 pubmed publisher
Woolsey C, Jankeel A, Matassov D, Geisbert J, Agans K, Borisevich V, et al. Immune correlates of postexposure vaccine protection against Marburg virus. Sci Rep. 2020;10:3071 pubmed publisher
Mansouri S, Katikaneni D, Gogoi H, Pipkin M, Machuca T, Emtiazjoo A, et al. Lung IFNAR1hi TNFR2+ cDC2 promotes lung regulatory T cells induction and maintains lung mucosal tolerance at steady state. Mucosal Immunol. 2020;13:595-608 pubmed publisher
D Antoni M, Kallianpur K, Premeaux T, Corley M, Fujita T, Laws E, et al. Lower Interferon Regulatory Factor-8 Expression in Peripheral Myeloid Cells Tracks With Adverse Central Nervous System Outcomes in Treated HIV Infection. Front Immunol. 2019;10:2789 pubmed publisher
Brown C, Gudjonson H, Pritykin Y, Deep D, Lavallée V, Mendoza A, et al. Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity. Cell. 2019;179:846-863.e24 pubmed publisher
Woolsey C, Menicucci A, Cross R, Luthra P, Agans K, Borisevich V, et al. A VP35 Mutant Ebola Virus Lacks Virulence but Can Elicit Protective Immunity to Wild-Type Virus Challenge. Cell Rep. 2019;28:3032-3046.e6 pubmed publisher
Wosen J, Ilstad Minnihan A, Co J, Jiang W, Mukhopadhyay D, Fernandez Becker N, et al. Human Intestinal Enteroids Model MHC-II in the Gut Epithelium. Front Immunol. 2019;10:1970 pubmed publisher
Vaccari M, Fourati S, Brown D, Silva de Castro I, Bissa M, Schifanella L, et al. Myeloid Cell Crosstalk Regulates the Efficacy of the DNA/ALVAC/gp120 HIV Vaccine Candidate. Front Immunol. 2019;10:1072 pubmed publisher
Kristoff J, Palma M, Garcia Bates T, Shen C, Sluis Cremer N, Gupta P, et al. Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1. EBioMedicine. 2019;43:295-306 pubmed publisher
Walk J, de Bree L, Graumans W, Stoter R, van Gemert G, van de Vegte Bolmer M, et al. Outcomes of controlled human malaria infection after BCG vaccination. Nat Commun. 2019;10:874 pubmed publisher
Hakimi A, Voss M, Kuo F, Sanchez A, Liu M, Nixon B, et al. Transcriptomic Profiling of the Tumor Microenvironment Reveals Distinct Subgroups of Clear Cell Renal Cell Cancer: Data from a Randomized Phase III Trial. Cancer Discov. 2019;9:510-525 pubmed publisher
Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed publisher
Kuo H, Ahmad R, Lee G, Gao C, Chen H, Ouyang Z, et al. Anti-apoptotic Protein BIRC5 Maintains Survival of HIV-1-Infected CD4+ T Cells. Immunity. 2018;48:1183-1194.e5 pubmed publisher
Vaccari M, Fourati S, Gordon S, Brown D, Bissa M, Schifanella L, et al. HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition. Nat Med. 2018;24:847-856 pubmed publisher
Kubo M, Satoh T, Ishiyama H, Tabata K, Tsumura H, Komori S, et al. Enhanced activated T cell subsets in prostate cancer patients receiving iodine-125 low-dose-rate prostate brachytherapy. Oncol Rep. 2018;39:417-424 pubmed publisher
Ishizaka A, Sato H, Nakamura H, Koga M, Kikuchi T, Hosoya N, et al. Short Intracellular HIV-1 Transcripts as Biomarkers of Residual Immune Activation in Patients on Antiretroviral Therapy. J Virol. 2016;90:5665-5676 pubmed publisher
Ball M, Shipman E, Kim H, Liby K, Pioli P. CDDO-Me Redirects Activation of Breast Tumor Associated Macrophages. PLoS ONE. 2016;11:e0149600 pubmed publisher
Lundberg K, Rydnert F, Broos S, Andersson M, Greiff L, Lindstedt M. Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets. PLoS ONE. 2016;11:e0148838 pubmed publisher
Vogel S, Grabski E, Buschjäger D, Klawonn F, Döring M, Wang J, et al. Antibody induced CD4 down-modulation of T cells is site-specifically mediated by CD64(+) cells. Sci Rep. 2015;5:18308 pubmed publisher
de Castro Amarante M, Pise Masison C, McKinnon K, Washington Parks R, Galli V, Omsland M, et al. Human T Cell Leukemia Virus Type 1 Infection of the Three Monocyte Subsets Contributes to Viral Burden in Humans. J Virol. 2015;90:2195-207 pubmed publisher
Nicholas K, Greenplate A, Flaherty D, Matlock B, Juan J, Smith R, et al. Multiparameter analysis of stimulated human peripheral blood mononuclear cells: A comparison of mass and fluorescence cytometry. Cytometry A. 2016;89:271-80 pubmed publisher
Kurose K, Ohue Y, Wada H, Iida S, Ishida T, Kojima T, et al. Phase Ia Study of FoxP3+ CD4 Treg Depletion by Infusion of a Humanized Anti-CCR4 Antibody, KW-0761, in Cancer Patients. Clin Cancer Res. 2015;21:4327-36 pubmed publisher
Finotti G, Tamassia N, Calzetti F, Fattovich G, Cassatella M. Endogenously produced TNF-α contributes to the expression of CXCL10/IP-10 in IFN-λ3-activated plasmacytoid dendritic cells. J Leukoc Biol. 2016;99:107-19 pubmed publisher
Teirlinck A, Roestenberg M, Bijker E, Hoffman S, Sauerwein R, Scholzen A. Plasmodium falciparum Infection of Human Volunteers Activates Monocytes and CD16+ Dendritic Cells and Induces Upregulation of CD16 and CD1c Expression. Infect Immun. 2015;83:3732-9 pubmed publisher
Ohue Y, Kurose K, Mizote Y, Matsumoto H, Nishio Y, Isobe M, et al. Prolongation of overall survival in advanced lung adenocarcinoma patients with the XAGE1 (GAGED2a) antibody. Clin Cancer Res. 2014;20:5052-63 pubmed publisher
Lauterbach N, Crivello P, Wieten L, Zito L, Groeneweg M, Voorter C, et al. Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain. Mol Immunol. 2014;59:19-29 pubmed publisher
Reismann M, Ghaffarpour N, Luvall E, Jirmo A, Winqvist O, Radtke J, et al. Dynamic Toll-like receptor expression predicts outcome of sclerotherapy for lymphatic malformations with OK-432 in children. J Surg Res. 2014;187:197-201 pubmed publisher
Donini M, Marongiu L, Fontana E, Dusi S. Prostate carcinoma cells LNCaP and glucan cooperate in induction of cytokine synthesis by dendritic cells: effect on natural killer cells and CD4+ lymphocytes activation. Prostate. 2012;72:566-76 pubmed publisher
Gonçalves R, Salmazi K, Santos B, Bastos M, Rocha S, Boscardin S, et al. CD4+ CD25+ Foxp3+ regulatory T cells, dendritic cells, and circulating cytokines in uncomplicated malaria: do different parasite species elicit similar host responses?. Infect Immun. 2010;78:4763-72 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Everts B, Adegnika A, Kruize Y, Smits H, Kremsner P, Yazdanbakhsh M. Functional impairment of human myeloid dendritic cells during Schistosoma haematobium infection. PLoS Negl Trop Dis. 2010;4:e667 pubmed publisher
Fujita H, Nograles K, Kikuchi T, Gonzalez J, Carucci J, Krueger J. Human Langerhans cells induce distinct IL-22-producing CD4+ T cells lacking IL-17 production. Proc Natl Acad Sci U S A. 2009;106:21795-800 pubmed publisher
Zaba L, Cardinale I, Gilleaudeau P, Sullivan Whalen M, Suárez Fariñas M, Fuentes Duculan J, et al. Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses. J Exp Med. 2007;204:3183-94 pubmed
Demirel O, Waibler Z, Kalinke U, Grunebach F, Appel S, Brossart P, et al. Identification of a lysosomal peptide transport system induced during dendritic cell development. J Biol Chem. 2007;282:37836-43 pubmed
product information
Antigen :
HLA-DR
Apps. Abbrev. :
Flow Cytometry
Cat # :
307618
Clone :
L243
Item :
APC/Cyanine7 anti-human HLA-DR
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class II, MHC class II
Size :
100 tests
Price (USD) :
292 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC_CYANINE7
Application Notes :
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a heterodimer. 19 Additional reported applications (for the relevant formats) include: immunoprecipitation 8 , Western blotting 8 , in vitro blocking of mixed lymphocyte reactions 9,10 , depeletion of MHC class II cells 7 , immunohistochemical staining of acetone-fixed frozen sections 4,5 , and spatial biology (IBEX) 21,22 . For sensitive functional assays, we recommend using the Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 307648, 307665 - 307669).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA