product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-human HLA-DR
catalog :
307615
quantity :
25 tests
price :
153 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE/Cy7
clone name :
L243
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 28
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:20; fig 2
Johnson D, Estrada M, Salgado R, Sanchez V, Doxie D, Opalenik S, et al. Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy. Nat Commun. 2016;7:10582 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Zhao X, Li J, Zheng L, Yang Q, Chen X, Chen X, et al. Immune Response on Optimal Timing and Fractionation Dose for Hypofractionated Radiotherapy in Non-Small-Cell Lung Cancer. Front Mol Biosci. 2022;9:786864 pubmed publisher
Platten M, Bunse L, Wick A, Bunse T, Le Cornet L, Harting I, et al. A vaccine targeting mutant IDH1 in newly diagnosed glioma. Nature. 2021;592:463-468 pubmed publisher
Gibellini L, De Biasi S, Paolini A, Borella R, Boraldi F, Mattioli M, et al. Altered bioenergetics and mitochondrial dysfunction of monocytes in patients with COVID-19 pneumonia. EMBO Mol Med. 2020;12:e13001 pubmed publisher
Garrido Martin E, Mellows T, Clarke J, Ganesan A, Wood O, Cazaly A, et al. M1hot tumor-associated macrophages boost tissue-resident memory T cells infiltration and survival in human lung cancer. J Immunother Cancer. 2020;8: pubmed publisher
Mothe B, Rosás Umbert M, Coll P, Manzardo C, Puertas M, Morón López S, et al. HIVconsv Vaccines and Romidepsin in Early-Treated HIV-1-Infected Individuals: Safety, Immunogenicity and Effect on the Viral Reservoir (Study BCN02). Front Immunol. 2020;11:823 pubmed publisher
Martin Gayo E, Gao C, Chen H, Ouyang Z, Kim D, Kolb K, et al. Immunological Fingerprints of Controllers Developing Neutralizing HIV-1 Antibodies. Cell Rep. 2020;30:984-996.e4 pubmed publisher
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
Domingos Pereira S, Sathiyanadan K, La Rosa S, Polák L, Chevalier M, Martel P, et al. Intravesical Ty21a Vaccine Promotes Dendritic Cells and T Cell-Mediated Tumor Regression in the MB49 Bladder Cancer Model. Cancer Immunol Res. 2019;: pubmed publisher
Zhang M, Fu Z, Chen J, Zhu B, Cheng Y, Fu L. Low level expression of the Mitochondrial Antiviral Signaling protein (MAVS) associated with long-term nonprogression in SIV-infected rhesus macaques. Virol J. 2018;15:159 pubmed publisher
Jelcić I, Al Nimer F, Wang J, Lentsch V, Planas R, Jelcic I, et al. Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis. Cell. 2018;175:85-100.e23 pubmed publisher
Zhu Y, Padgett L, Dinh H, Marcovecchio P, Blatchley A, Wu R, et al. Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow. Cell Rep. 2018;24:2329-2341.e8 pubmed publisher
Mastoridis S, Bertolino G, Whitehouse G, Dazzi F, Sanchez Fueyo A, Martinez Llordella M. Multiparametric Analysis of Circulating Exosomes and Other Small Extracellular Vesicles by Advanced Imaging Flow Cytometry. Front Immunol. 2018;9:1583 pubmed publisher
Alissafi T, Hatzioannou A, Mintzas K, Barouni R, Banos A, Sormendi S, et al. Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells. J Clin Invest. 2018;128:3840-3852 pubmed publisher
Orengo J, Radin A, Kamat V, Badithe A, Ben L, Bennett B, et al. Treating cat allergy with monoclonal IgG antibodies that bind allergen and prevent IgE engagement. Nat Commun. 2018;9:1421 pubmed publisher
Neff C, Krueger O, Xiong K, Arif S, Nusbacher N, Schneider J, et al. Fecal Microbiota Composition Drives Immune Activation in HIV-infected Individuals. EBioMedicine. 2018;30:192-202 pubmed publisher
Du X, Tang F, Liu M, Su J, Zhang Y, Wu W, et al. A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy. Cell Res. 2018;28:416-432 pubmed publisher
Luo N, Nixon M, Gonzalez Ericsson P, Sanchez V, Opalenik S, Li H, et al. DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer. Nat Commun. 2018;9:248 pubmed publisher
Barman S, Kayama H, Okuzaki D, Ogino T, Osawa H, Matsuno H, et al. Identification of a human intestinal myeloid cell subset that regulates gut homeostasis. Int Immunol. 2016;28:533-545 pubmed
Pennington L, Tarchevskaya S, Brigger D, Sathiyamoorthy K, Graham M, Nadeau K, et al. Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange. Nat Commun. 2016;7:11610 pubmed publisher
Macal M, Tam M, Hesser C, Di Domizio J, Léger P, Gilliet M, et al. CD28 Deficiency Enhances Type I IFN Production by Murine Plasmacytoid Dendritic Cells. J Immunol. 2016;196:1900-9 pubmed publisher
Li Causi E, Parikh S, Chudley L, Layfield D, Ottensmeier C, Stevenson F, et al. Vaccination Expands Antigen-Specific CD4+ Memory T Cells and Mobilizes Bystander Central Memory T Cells. PLoS ONE. 2015;10:e0136717 pubmed publisher
Lauterbach N, Crivello P, Wieten L, Zito L, Groeneweg M, Voorter C, et al. Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain. Mol Immunol. 2014;59:19-29 pubmed publisher
Gonçalves R, Salmazi K, Santos B, Bastos M, Rocha S, Boscardin S, et al. CD4+ CD25+ Foxp3+ regulatory T cells, dendritic cells, and circulating cytokines in uncomplicated malaria: do different parasite species elicit similar host responses?. Infect Immun. 2010;78:4763-72 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Fujita H, Nograles K, Kikuchi T, Gonzalez J, Carucci J, Krueger J. Human Langerhans cells induce distinct IL-22-producing CD4+ T cells lacking IL-17 production. Proc Natl Acad Sci U S A. 2009;106:21795-800 pubmed publisher
Zaba L, Cardinale I, Gilleaudeau P, Sullivan Whalen M, Suárez Fariñas M, Fuentes Duculan J, et al. Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses. J Exp Med. 2007;204:3183-94 pubmed
product information
Antigen :
HLA-DR
Apps. Abbrev. :
Flow Cytometry
Cat # :
307615
Clone :
L243
Item :
PE/Cyanine7 anti-human HLA-DR
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class II, MHC class II
Size :
25 tests
Price (USD) :
153 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE_CYANINE7
Application Notes :
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a heterodimer. 19 Additional reported applications (for the relevant formats) include: immunoprecipitation 8 , Western blotting 8 , in vitro blocking of mixed lymphocyte reactions 9,10 , depeletion of MHC class II cells 7 , immunohistochemical staining of acetone-fixed frozen sections 4,5 , and spatial biology (IBEX) 21,22 . For sensitive functional assays, we recommend using the Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 307648, 307665 - 307669).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA