LEAF™ Purified anti-human HLA-DR | BioLegend 307611 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-human HLA-DR

catalog :

307611

quantity :

50 μg

price :

70 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

L243

reactivity :

human, mouse, dog, chimpanzee, Rhesus monkey

application :

western blot, immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, other

citations: 34

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1a	Richardson J, Armbruster N, Günter M, Henes J, Autenrieth S. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018;9:2603 pubmed publisher
flow cytometry; human; loading ...; fig 4b	Otsuka Y, Watanabe E, Shinya E, Okura S, Saeki H, Geijtenbeek T, et al. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells. J Immunol. 2018;201:3006-3016 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Walwyn Brown K, Guldevall K, Saeed M, Pende D, Önfelt B, MacDonald A, et al. Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1. J Immunol. 2018;201:2028-2041 pubmed publisher
flow cytometry; human; loading ...; fig s3b	Arnold I, Artola Borán M, Tallón de Lara P, Kyburz A, Taube C, OTTEMANN K, et al. Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation. J Exp Med. 2018;215:2055-2072 pubmed publisher
flow cytometry; human; loading ...; fig 1b	Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez Giraldez F, et al. Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018;3: pubmed publisher
flow cytometry; human; loading ...; fig 3b	Fujisaka Y, Iwata T, Tamai K, Nakamura M, Mochizuki M, Shibuya R, et al. Long non-coding RNA HOTAIR up-regulates chemokine (C-C motif) ligand 2 and promotes proliferation of macrophages and myeloid-derived suppressor cells in hepatocellular carcinoma cell lines. Oncol Lett. 2018;15:509-514 pubmed publisher
flow cytometry; human; fig 1a	Bujko A, Atlasy N, Landsverk O, Richter L, Yaqub S, Horneland R, et al. Transcriptional and functional profiling defines human small intestinal macrophage subsets. J Exp Med. 2018;215:441-458 pubmed publisher
flow cytometry; human; 1:200; loading ...; fig s1d	Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, et al. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement. Nat Commun. 2017;8:14253 pubmed publisher
flow cytometry; human; loading ...; fig 2b	O CONNOR D, Clutterbuck E, Thompson A, Snape M, Ramasamy M, Kelly D, et al. High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. Genome Med. 2017;9:11 pubmed publisher
flow cytometry; mouse; loading ...; fig 2d	Cheng L, Ma J, Li J, Li D, Li G, Li F, et al. Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs. J Clin Invest. 2017;127:269-279 pubmed publisher
flow cytometry; Rhesus monkey; loading ...; fig 7a	Kasturi S, Kozlowski P, Nakaya H, Burger M, Russo P, Pham M, et al. Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques. J Virol. 2017;91: pubmed publisher
flow cytometry; human; loading ...; tbl 1	Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
flow cytometry; human; loading ...	Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
flow cytometry; human; loading ...	Cerny D, Thi Le D, The T, Zuest R, Kg S, Velumani S, et al. Complete human CD1a deficiency on Langerhans cells due to a rare point mutation in the coding sequence. J Allergy Clin Immunol. 2016;138:1709-1712.e11 pubmed publisher
flow cytometry; human; loading ...; fig 2b	Gadd V, Patel P, Jose S, Horsfall L, Powell E, Irvine K. Altered Peripheral Blood Monocyte Phenotype and Function in Chronic Liver Disease: Implications for Hepatic Recruitment and Systemic Inflammation. PLoS ONE. 2016;11:e0157771 pubmed publisher
flow cytometry; human; loading ...; fig 7a	Zanetti S, Ziblat A, Torres N, Zwirner N, Bouzat C. Expression and Functional Role of ?7 Nicotinic Receptor in Human Cytokine-stimulated Natural Killer (NK) Cells. J Biol Chem. 2016;291:16541-52 pubmed publisher
flow cytometry; human; loading ...; fig 3	Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
flow cytometry; human; fig s6a	Srivastava P, Paluch B, Matsuzaki J, James S, Collamat Lai G, Blagitko Dorfs N, et al. Induction of cancer testis antigen expression in circulating acute myeloid leukemia blasts following hypomethylating agent monotherapy. Oncotarget. 2016;7:12840-56 pubmed publisher
flow cytometry; human; 1:20; fig 2	Johnson D, Estrada M, Salgado R, Sanchez V, Doxie D, Opalenik S, et al. Melanoma-specific MHC-II expression represents a tumour-autonomous phenotype and predicts response to anti-PD-1/PD-L1 therapy. Nat Commun. 2016;7:10582 pubmed publisher
other; human; loading ...; fig st1 flow cytometry; human; loading ...; fig st3	Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
flow cytometry; human	Rosario M, Liu B, Kong L, Collins L, Schneider S, Chen X, et al. The IL-15-Based ALT-803 Complex Enhances FcÎ³RIIIa-Triggered NK Cell Responses and In Vivo Clearance of B Cell Lymphomas. Clin Cancer Res. 2016;22:596-608 pubmed publisher
flow cytometry; human; fig 3	Yawata N, Selva K, Liu Y, Tan K, Lee A, Siak J, et al. Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection. Mucosal Immunol. 2016;9:159-70 pubmed publisher
flow cytometry; human; loading ...; fig s1b	Schulz A, MÃ¤lzer J, Domingo C, JÃ¼rchott K, GrÃ¼tzkau A, Babel N, et al. Low Thymic Activity and Dendritic Cell Numbers Are Associated with the Immune Response to Primary Viral Infection in Elderly Humans. J Immunol. 2015;195:4699-711 pubmed publisher
flow cytometry; human; tbl s1	Weinberg A, Muresan P, Richardson K, Fenton T, Domínguez T, Bloom A, et al. Determinants of vaccine immunogenicity in HIV-infected pregnant women: analysis of B and T cell responses to pandemic H1N1 monovalent vaccine. PLoS ONE. 2015;10:e0122431 pubmed publisher
flow cytometry; human	Hautefort A, Girerd B, Montani D, Cohen Kaminsky S, Price L, Lambrecht B, et al. T-helper 17 cell polarization in pulmonary arterial hypertension. Chest. 2015;147:1610-1620 pubmed publisher
flow cytometry; human; loading ...; fig 5	Weed D, Vella J, Reis I, De La Fuente A, Gomez C, Sargi Z, et al. Tadalafil reduces myeloid-derived suppressor cells and regulatory T cells and promotes tumor immunity in patients with head and neck squamous cell carcinoma. Clin Cancer Res. 2015;21:39-48 pubmed publisher
flow cytometry; human; fig 3	Rogacev K, Zawada A, Hundsdorfer J, Achenbach M, Held G, Fliser D, et al. Immunosuppression and monocyte subsets. Nephrol Dial Transplant. 2015;30:143-53 pubmed publisher
flow cytometry; human	Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
	Chang S, Park C. Allogeneic ADSCs induce CD8 T cell-mediated cytotoxicity and faster cell death after exposure to xenogeneic serum or proinflammatory cytokines. Exp Mol Med. 2019;51:28 pubmed publisher
	Casey N, Fujiwara H, Tanimoto K, Okamoto S, Mineno J, Kuzushima K, et al. A Functionally Superior Second-Generation Vector Expressing an Aurora Kinase-A-Specific T-Cell Receptor for Anti-Leukaemia Adoptive Immunotherapy. PLoS ONE. 2016;11:e0156896 pubmed publisher
	Lauterbach N, Crivello P, Wieten L, Zito L, Groeneweg M, Voorter C, et al. Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain. Mol Immunol. 2014;59:19-29 pubmed publisher
	Narita T, Ishida T, Masaki A, Suzuki S, Ito A, Mori F, et al. HTLV-1 bZIP factor-specific CD4 T cell responses in adult T cell leukemia/lymphoma patients after allogeneic hematopoietic stem cell transplantation. J Immunol. 2014;192:940-7 pubmed publisher
	Dillon S, Manuzak J, Leone A, Lee E, Rogers L, McCarter M, et al. HIV-1 infection of human intestinal lamina propria CD4+ T cells in vitro is enhanced by exposure to commensal Escherichia coli. J Immunol. 2012;189:885-96 pubmed publisher
	Chang Y, Chen T, Lee C, Yang C, Wang H, Wang C, et al. Epigenetic control of MHC class II expression in tumor-associated macrophages by decoy receptor 3. Blood. 2008;111:5054-63 pubmed publisher

product information

Antigen :

HLA-DR

Apps. Abbrev. :

Flow Cytometry, CyTOF®, Block, IHC, IP, Western Blotting

Cat # :

307611

Clone :

L243

Item :

LEAF™ Purified anti-human HLA-DR

Isotype :

Mouse IgG2a, κ

Other Names :

Major Histocompatibility Class II, MHC class II

Size :

50 μg

Price (USD) :

70 USD

Reactivity :

Human, African Green, Baboon, Chimpanzee, Cynomolgus, Dog (Canine), Marmoset, Rhesus, Squirrel Monkey, Tamarin

Clonality :

Monoclonal

Host :

Mouse

Conjugate/Tag/Label :

LEAF

Application Notes :

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DR which depends on the correct folding of the heterodimer. 19 Additional reported applications (for the relevant formats) include: immunoprecipitation 8 , Western blotting 8 , in vitro blocking of mixed lymphocyte reactions 9,10 , depeletion of MHC class II cells 7 , and immunohistochemical staining of acetone-fixed frozen sections 4,5 . For sensitive functional assays, we recommend using the Ultra-LEAF purified antibody (Cat. No. 307648).