product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-human HLA-DR
catalog :
307609
quantity :
25 tests
price :
83 USD
clonality :
monoclonal
host :
mouse
conjugate :
APC
clone name :
L243
reactivity :
human, mouse, rhesus macaque
application :
flow cytometry
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; 1:100; loading ...; fig 6b, s26
Liu Y, Wang L, Song Q, Ali M, Crowe W, Kucera G, et al. Intrapleural nano-immunotherapy promotes innate and adaptive immune responses to enhance anti-PD-L1 therapy for malignant pleural effusion. Nat Nanotechnol. 2022;17:206-216 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Poncette L, Chen X, Lorenz F, Blankenstein T. Effective NY-ESO-1-specific MHC II-restricted T cell receptors from antigen-negative hosts enhance tumor regression. J Clin Invest. 2019;129:324-335 pubmed publisher
  • flow cytometry; human; loading ...; fig 3
Zwang N, Zhang R, Germana S, Fan M, Hastings W, Cao A, et al. Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways. Am J Transplant. 2016;16:2624-38 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Wu H, Zhu R, Zheng B, Liao G, Wang F, Ding J, et al. Single-Cell Sequencing Reveals an Intrinsic Heterogeneity of the Preovulatory Follicular Microenvironment. Biomolecules. 2022;12: pubmed publisher
Wu L, Xu Y, Zhao H, Zhou Y, Chen Y, Yang S, et al. FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape. Theranostics. 2022;12:842-858 pubmed publisher
Loo Yau H, De Carvalho D. Measuring the effect of drug treatments on primary human CD8+ T cell activation and cytolytic potential. STAR Protoc. 2021;2:100549 pubmed publisher
Ramaswamy A, Brodsky N, Sumida T, Comi M, Asashima H, Hoehn K, et al. Immune dysregulation and autoreactivity correlate with disease severity in SARS-CoV-2-associated multisystem inflammatory syndrome in children. Immunity. 2021;54:1083-1095.e7 pubmed publisher
Cai J, Gao H, Zhao J, Hu S, Liang X, Yang Y, et al. Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4+ T cells. elife. 2021;10: pubmed publisher
Wenthe J, Naseri S, Labani Motlagh A, Enblad G, Wikstrom K, Eriksson E, et al. Boosting CAR T-cell responses in lymphoma by simultaneous targeting of CD40/4-1BB using oncolytic viral gene therapy. Cancer Immunol Immunother. 2021;70:2851-2865 pubmed publisher
Cho J, Okuma A, Sofjan K, Lee S, Collins J, Wong W. Engineering advanced logic and distributed computing in human CAR immune cells. Nat Commun. 2021;12:792 pubmed publisher
Rodda L, Netland J, Shehata L, Pruner K, Morawski P, Thouvenel C, et al. Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19. Cell. 2021;184:169-183.e17 pubmed publisher
Holokai L, Chakrabarti J, Lundy J, Croagh D, Adhikary P, Richards S, et al. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma. Cancers (Basel). 2020;12: pubmed publisher
Mączyńska J, Da Pieve C, Burley T, Raes F, Shah A, Saczko J, et al. Immunomodulatory activity of IR700-labelled affibody targeting HER2. Cell Death Dis. 2020;11:886 pubmed publisher
Nazari Shafti T, Neuber S, Duran A, Exarchos V, Beez C, Meyborg H, et al. MiRNA Profiles of Extracellular Vesicles Secreted by Mesenchymal Stromal Cells-Can They Predict Potential Off-Target Effects?. Biomolecules. 2020;10: pubmed publisher
Leite N, Sintov E, Meissner T, Brehm M, Greiner D, Harlan D, et al. Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells. Cell Rep. 2020;32:107894 pubmed publisher
Katsuyama E, Suárez Fueyo A, Bradley S, Mizui M, Marin A, Mulki L, et al. The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections. Cell Rep. 2020;30:112-123.e4 pubmed publisher
Del Alcazar D, Wang Y, He C, Wendel B, Del Rio Estrada P, Lin J, et al. Mapping the Lineage Relationship between CXCR5+ and CXCR5- CD4+ T Cells in HIV-Infected Human Lymph Nodes. Cell Rep. 2019;28:3047-3060.e7 pubmed publisher
Wang Y, Chen C, Qi J, Wu F, Guan J, Chen Z, et al. Altered PGE2-EP2 is associated with an excessive immune response in HBV-related acute-on-chronic liver failure. J Transl Med. 2019;17:93 pubmed publisher
Walk J, de Bree L, Graumans W, Stoter R, van Gemert G, van de Vegte Bolmer M, et al. Outcomes of controlled human malaria infection after BCG vaccination. Nat Commun. 2019;10:874 pubmed publisher
Jin Y, Roberts G, Ferrara T, Ben S, van Geel N, Wolkerstorfer A, et al. Early-onset autoimmune vitiligo associated with an enhancer variant haplotype that upregulates class II HLA expression. Nat Commun. 2019;10:391 pubmed publisher
Saraiva D, Jacinto A, Borralho P, Braga S, Cabral M. HLA-DR in Cytotoxic T Lymphocytes Predicts Breast Cancer Patients' Response to Neoadjuvant Chemotherapy. Front Immunol. 2018;9:2605 pubmed publisher
Roy Chowdhury R, Vallania F, Yang Q, Lopez Angel C, Darboe F, Penn Nicholson A, et al. A multi-cohort study of the immune factors associated with M. tuberculosis infection outcomes. Nature. 2018;560:644-648 pubmed publisher
Sasano T, Mabuchi S, Kozasa K, Kuroda H, Kawano M, Takahashi R, et al. The Highly Metastatic Nature of Uterine Cervical/Endometrial Cancer Displaying Tumor-Related Leukocytosis: Clinical and Preclinical Investigations. Clin Cancer Res. 2018;24:4018-4029 pubmed publisher
Vielle N, Zumkehr B, García Nicolás O, Blank F, Stojanov M, Musso D, et al. Silent infection of human dendritic cells by African and Asian strains of Zika virus. Sci Rep. 2018;8:5440 pubmed publisher
Yuan Z, Kang G, Daharsh L, Fan W, Li Q. SIVcpz closely related to the ancestral HIV-1 is less or non-pathogenic to humans in a hu-BLT mouse model. Emerg Microbes Infect. 2018;7:59 pubmed publisher
Yang M, Xu J, Wang Q, Zhang A, Wang K. An obligatory anaerobic Salmonella typhimurium strain redirects M2 macrophages to the M1 phenotype. Oncol Lett. 2018;15:3918-3922 pubmed publisher
Eitas T, Stepp W, Sjeklocha L, Long C, Riley C, Callahan J, et al. Differential regulation of innate immune cytokine production through pharmacological activation of Nuclear Factor-Erythroid-2-Related Factor 2 (NRF2) in burn patient immune cells and monocytes. PLoS ONE. 2017;12:e0184164 pubmed publisher
Singh N, Herbert B, Sooranna G, Orsi N, Edey L, Dasgupta T, et al. Is myometrial inflammation a cause or a consequence of term human labour?. J Endocrinol. 2017;235:69-83 pubmed publisher
Kotsiou E, Okosun J, Besley C, Iqbal S, Matthews J, Fitzgibbon J, et al. TNFRSF14 aberrations in follicular lymphoma increase clinically significant allogeneic T-cell responses. Blood. 2016;128:72-81 pubmed publisher
Alfaro C, Teijeira A, Oñate C, Perez G, Sanmamed M, Andueza M, et al. Tumor-Produced Interleukin-8 Attracts Human Myeloid-Derived Suppressor Cells and Elicits Extrusion of Neutrophil Extracellular Traps (NETs). Clin Cancer Res. 2016;22:3924-36 pubmed publisher
Scottà C, Fanelli G, Hoong S, Romano M, Lamperti E, Sukthankar M, et al. Impact of immunosuppressive drugs on the therapeutic efficacy of ex vivo expanded human regulatory T cells. Haematologica. 2016;101:91-100 pubmed publisher
Bsat M, Chapuy L, Baba N, Rubio M, Panzini B, Wassef R, et al. Differential accumulation and function of proinflammatory 6-sulfo LacNAc dendritic cells in lymph node and colon of Crohn's versus ulcerative colitis patients. J Leukoc Biol. 2015;98:671-81 pubmed publisher
Metcalf Pate K, Pohlmeyer C, Walker Sperling V, Foote J, Najarro K, Cryer C, et al. A Murine Viral Outgrowth Assay to Detect Residual HIV Type 1 in Patients With Undetectable Viral Loads. J Infect Dis. 2015;212:1387-96 pubmed publisher
Li L, Zhang T, Diao W, Jin F, Shi L, Meng J, et al. Role of Myeloid-Derived Suppressor Cells in Glucocorticoid-Mediated Amelioration of FSGS. J Am Soc Nephrol. 2015;26:2183-97 pubmed publisher
Li Z, Li W, Li N, Jiao Y, Chen D, Cui L, et al. γδ T cells are involved in acute HIV infection and associated with AIDS progression. PLoS ONE. 2014;9:e106064 pubmed publisher
Lauterbach N, Crivello P, Wieten L, Zito L, Groeneweg M, Voorter C, et al. Allorecognition of HLA-DP by CD4+ T cells is affected by polymorphism in its alpha chain. Mol Immunol. 2014;59:19-29 pubmed publisher
Zizzo G, Hilliard B, Monestier M, Cohen P. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20 pubmed
product information
Antigen :
HLA-DR
Apps. Abbrev. :
Flow Cytometry
Cat # :
307609
Clone :
L243
Item :
APC anti-human HLA-DR
Isotype :
Mouse IgG2a, κ
Other Names :
Major Histocompatibility Class II, MHC class II
Size :
25 tests
Price (USD) :
83 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
APC
Application Notes :
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the a heterodimer. 19 Additional reported applications (for the relevant formats) include: immunoprecipitation 8 , Western blotting 8 , in vitro blocking of mixed lymphocyte reactions 9,10 , depeletion of MHC class II cells 7 , immunohistochemical staining of acetone-fixed frozen sections 4,5 , and spatial biology (IBEX) 21,22 . For sensitive functional assays, we recommend using the Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 307648, 307665 - 307669).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA