product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-human CD184 (CXCR4)
catalog :
306505
quantity :
25 tests
price :
129 USD
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
12G5
reactivity :
human, rhesus macaque
application :
flow cytometry
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s4e
Wu T, Wang W, Shi G, Hao M, Wang Y, Yao M, et al. Targeting HIC1/TGF-β axis-shaped prostate cancer microenvironment restrains its progression. Cell Death Dis. 2022;13:624 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig s4b
Smith N, Pietrancosta N, Davidson S, Dutrieux J, Chauveau L, Cutolo P, et al. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement. Nat Commun. 2017;8:14253 pubmed publisher
  • flow cytometry; human
Anderson E, Mooney D. The Combination of Vascular Endothelial Growth Factor and Stromal Cell-Derived Factor Induces Superior Angiogenic Sprouting by Outgrowth Endothelial Cells. J Vasc Res. 2015;52:62-9 pubmed publisher
  • flow cytometry; human
Mandl M, Schmitz S, Weber C, Hristov M. Characterization of the CD14++CD16+ monocyte population in human bone marrow. PLoS ONE. 2014;9:e112140 pubmed publisher
  • flow cytometry; human
Fuentes T, Appleby N, Tsay E, Martinez J, Bailey L, Hasaniya N, et al. Human neonatal cardiovascular progenitors: unlocking the secret to regenerative ability. PLoS ONE. 2013;8:e77464 pubmed publisher
  • flow cytometry; human
Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R, et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011;332:687-96 pubmed publisher
Corral Jara K, Chauvin C, Abou Jaoudé W, Grandclaudon M, Naldi A, Soumelis V, et al. Interplay between SMAD2 and STAT5A is a critical determinant of IL-17A/IL-17F differential expression. Mol Biomed. 2021;2:9 pubmed publisher
Houtsma R, Hogeling S, Schuringa J. CombiFlow: Flow cytometry-based identification and characterization of genetically and functionally distinct AML subclones. STAR Protoc. 2021;2:100864 pubmed publisher
McQuade A, Kang Y, Hasselmann J, Jairaman A, Sotelo A, Coburn M, et al. Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer's disease. Nat Commun. 2020;11:5370 pubmed publisher
Yang C, Chen F, Ren P, Lofchy L, Wan C, Shen J, et al. Delivery of a model lipophilic membrane cargo to bone marrow via cell-derived microparticles. J Control Release. 2020;326:324-334 pubmed publisher
Agha O, Mueller Immergluck A, Liu M, Zhang H, Theologis A, Clark A, et al. Intervertebral disc herniation effects on multifidus muscle composition and resident stem cell populations. JOR Spine. 2020;3:e1091 pubmed publisher
Yang W, Lampe P, Kensel Hammes P, Hesson J, Ware C, Crisa L, et al. Connexin 43 Functions as a Positive Regulator of Stem Cell Differentiation into Definitive Endoderm and Pancreatic Progenitors. iScience. 2019;19:450-460 pubmed publisher
Mair B, Tomic J, Masud S, Tonge P, Weiss A, Usaj M, et al. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Cell Rep. 2019;27:599-615.e12 pubmed publisher
Chen H, Poran A, Unni A, Huang S, Elemento O, Snoeck H, et al. Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells. J Exp Med. 2019;216:674-687 pubmed publisher
Zhang Y, Yang Y, Jiang M, Huang S, Zhang W, Al Alam D, et al. 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling. Cell Stem Cell. 2018;23:516-529.e5 pubmed publisher
Idorn M, Olsen M, Halldórsdóttir H, Skadborg S, Pedersen M, Høgdall C, et al. Improved migration of tumor ascites lymphocytes to ovarian cancer microenvironment by CXCR2 transduction. Oncoimmunology. 2018;7:e1412029 pubmed publisher
Muir L, McKay P, Petrova V, Klymenko O, Kratochvil S, Pinder C, et al. Optimisation of ex vivo memory B cell expansion/differentiation for interrogation of rare peripheral memory B cell subset responses. Wellcome Open Res. 2017;2:97 pubmed publisher
Jung Y, Cackowski F, Yumoto K, Decker A, Wang J, Kim J, et al. CXCL12γ Promotes Metastatic Castration-Resistant Prostate Cancer by Inducing Cancer Stem Cell and Neuroendocrine Phenotypes. Cancer Res. 2018;78:2026-2039 pubmed publisher
Nevins A, Marchese A. Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4. Methods Mol Biol. 2018;1722:151-164 pubmed publisher
Suan D, Kräutler N, Mååg J, Butt D, Bourne K, Hermes J, et al. CCR6 Defines Memory B Cell Precursors in Mouse and Human Germinal Centers, Revealing Light-Zone Location and Predominant Low Antigen Affinity. Immunity. 2017;47:1142-1153.e4 pubmed publisher
Magri G, Comerma L, Pybus M, Sintes J, Lligé D, Segura Garzón D, et al. Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals. Immunity. 2017;47:118-134.e8 pubmed publisher
Chittasupho C, Anuchapreeda S, Sarisuta N. CXCR4 targeted dendrimer for anti-cancer drug delivery and breast cancer cell migration inhibition. Eur J Pharm Biopharm. 2017;119:310-321 pubmed publisher
Xu J, Liang J, Meng Y, Yan J, Yu X, Liu C, et al. Vascular CXCR4 Expression Promotes Vessel Sprouting and Sensitivity to Sorafenib Treatment in Hepatocellular Carcinoma. Clin Cancer Res. 2017;23:4482-4492 pubmed publisher
Alekhina O, Marchese A. ?-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4. J Biol Chem. 2016;291:26083-26097 pubmed
Yuan Z, Lourenco S, Sage E, Kolluri K, Lowdell M, Janes S. Cryopreservation of human mesenchymal stromal cells expressing TRAIL for human anti-cancer therapy. Cytotherapy. 2016;18:860-9 pubmed publisher
Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016;196:4064-74 pubmed publisher
Siewiera J, Gouilly J, Hocine H, Cartron G, Levy C, Al Daccak R, et al. Natural cytotoxicity receptor splice variants orchestrate the distinct functions of human natural killer cell subtypes. Nat Commun. 2015;6:10183 pubmed publisher
Hou P, Chen S, Wang S, Yu X, Chen Y, Jiang M, et al. Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection. Sci Rep. 2015;5:15577 pubmed publisher
Yin S, Mao Y, Li X, Yue C, Zhou C, Huang L, et al. Hyperactivation and in situ recruitment of inflammatory Vδ2 T cells contributes to disease pathogenesis in systemic lupus erythematosus. Sci Rep. 2015;5:14432 pubmed publisher
Wang H, Luo X, Yao L, Lehman D, Wang P. Improvement of Cell Survival During Human Pluripotent Stem Cell Definitive Endoderm Differentiation. Stem Cells Dev. 2015;24:2536-46 pubmed publisher
Schneider T, Ehrig K, Liewert I, Alban S. Interference with the CXCL12/CXCR4 axis as potential antitumor strategy: superiority of a sulfated galactofucan from the brown alga Saccharina latissima and fucoidan over heparins. Glycobiology. 2015;25:812-24 pubmed publisher
Bettman N, Avivi I, Rosenbaum H, Bisharat L, Katz T. Impaired migration capacity in monocytes derived from patients with Gaucher disease. Blood Cells Mol Dis. 2015;55:180-6 pubmed publisher
Wang B, Wang W, Niu W, Liu E, Liu X, Wang J, et al. SDF-1/CXCR4 axis promotes directional migration of colorectal cancer cells through upregulation of integrin ?v?6. Carcinogenesis. 2014;35:282-91 pubmed publisher
Guo P, You J, Yang J, Moses M, Auguste D. Using breast cancer cell CXCR4 surface expression to predict liposome binding and cytotoxicity. Biomaterials. 2012;33:8104-10 pubmed publisher
Adikrisna R, Tanaka S, Muramatsu S, Aihara A, Ban D, Ochiai T, et al. Identification of pancreatic cancer stem cells and selective toxicity of chemotherapeutic agents. Gastroenterology. 2012;143:234-45.e7 pubmed publisher
Blengio F, Raggi F, Pierobon D, Cappello P, Eva A, Giovarelli M, et al. The hypoxic environment reprograms the cytokine/chemokine expression profile of human mature dendritic cells. Immunobiology. 2013;218:76-89 pubmed publisher
Hartmann T, Grabovsky V, Pasvolsky R, Shulman Z, Buss E, Spiegel A, et al. A crosstalk between intracellular CXCR7 and CXCR4 involved in rapid CXCL12-triggered integrin activation but not in chemokine-triggered motility of human T lymphocytes and CD34+ cells. J Leukoc Biol. 2008;84:1130-40 pubmed publisher
product information
Antigen :
CD184
Apps. Abbrev. :
FC
Cat # :
306505
Clone :
12G5
Item :
PE anti-human CD184 (CXCR4)
Isotype :
Mouse IgG2a, κ
Other Names :
CXCR4, Fusin
Size :
25 tests
Price (USD) :
129 USD
Reactivity :
Human, Cynomolgus, Rhesus
Clonality :
Monoclonal
Host :
Mouse
Conjugate/Tag/Label :
PE
Immunogen :
CP-MAC-infected Sup-T1 cells
Application Notes :
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections^11, immunocytochemistry^3, immunofluorescence microscopy^2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1^4,5. Clone 12G5 may not be suitable for Western blotting.^10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA