Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Ultra-LEAF™ Purified anti-human CD178 (FasL)

306416

1 mg

737 USD

monoclonal

mouse

nonconjugated

NOK-1

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

FC, ICC, IP, WB

306416

NOK-1

Ultra-LEAF™ Purified anti-human CD178 (FasL)

Mouse IgG1, κ

Fas Ligand (FasL), CD95L, TNFSF6, Fas-L

1 mg

737 USD

Human

Monoclonal

Mouse

ULEAF

L5178Y mouse T lymphoma cells expressing recombinant human FasL

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, immunofluorescence microscopy^3, immunocytochemistry^2, blocking of Fas induced apoptosis^1, and Western blotting^11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306415 and 306416).;